日本内分泌学会雑誌
Online ISSN : 2186-506X
Print ISSN : 0029-0661
ISSN-L : 0029-0661
37 巻, 1 号
選択された号の論文の7件中1~7を表示しています
  • 特に妊娠尿中よりの16-Epi-estriolの分離測定について
    志田 圭三, 木村 光比子, 神戸川 明
    1961 年 37 巻 1 号 p. 5-9,1
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
    A chromatographical method for fractionation of estrogen methyl ethers.
    100ml. of late pregnancy urine was boiled for 15 minutes with 15 vol. % of conc HCl. Hydrolyzed urine was extracted twice with 150 ml. of ether and the extract was washed twice with 25 ml. of 5% NaHCO3 solution to remove acid substances and then washed three times with water (20ml.). The ether solution was dried with anhydrous Na2SO4 and was distilled to about 10 ml. in a water bath.
    The contents were transferred together with 25 ml. of benzene and 25 ml. of petroleum ether into a separating funnel. The benzene petroleum ether solution was extracted twice with 25 ml. of 1.6% NaOH. Then H3BO3 (0.9 gr.) was added to the given NaOH extracts. The flask was placed in a 37°C water bath and 1 ml. of dimethylsulfate was added. The flask was stirred by a magnetic stirrer at 37°C for 30 minutes and was allowed to stand at room temperature overnight.
    Ten ml. of 20% NaOH and 2.5 ml. of 30% H2O2 were added to the flask and the contents of the flask were transferred to a separating funnel.
    The methylated estrogens were chromatographed by alumina column ; the size of the column was 0.5 * 20 cm. The column was prepared by partly filling it with petroleum ether and then adding the 2.0 gr. of Brockmann alumina (activity III). The chromatograms were run at 18°C under the pressure of 10-12 cm./Hg. The eluates was divided into 2ml. for each tube.
    Estrone methyl ester, estradiol methyl ester, 16-epi-estriol methyl ester and estriol methyl ester were eluated with 40% petroleum ether in benzene, benzen, 1.6% methanol in benzene and 3.0% methanol in benzene, respectively.
    By this method, estrogens in pregnant urine were separated into four fractions and were measured. It was then determined that the 16-epi-estriol contained in late prengnancy urine was 11.5% of the total estrogens.
  • 阿部 千秋
    1961 年 37 巻 1 号 p. 10-19,1
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
    Thyrotropin releasing factor (TRF) was injected into Wistar male rats and DD male mice for various durations in combination with various substances in order to produce experimentally, thyroid diseases clinically resembling simple goiter, hyperthyroidism, subacute thyroiditis and thyroid tumor.
    1. When the animals were fed a definite amount of iodine, and at the same time injected daily with TRF for 3 months, the thyroid function was lowered with an increase in thyroid weight, thus presenting a clinical picture of simple goiter.
    2. When animals were fed beans, and at the same time injected daily with TRF for 3 months, hyperthyroidism without thyroid weight gain was produced.
    3. When Freund's adjuvant, thyroglobulin and TRF were daily and concurrently administered for 1 month, histological findings of subacute thyroiditis, such as infiltration of lymphocytes and plasma cells, degeneration of follicular epithelium, destruction of follicles, and outflow of colloid from follicles, were observed.
    4. When TRF was injected daily for 3 months, thyroid function and thyroid weight increased nearly in parallel. In the case of daily injection for a six-months period, hyperfunction of the thyroid was stopped, and only hypertrophy and proliferation of the gland became remarkable. Namely, segregation of morphology from function was observed. Histologically there was a picture reminiscent of tumor.
  • 尿中副甲状腺ホルモンの一新測定法に就て
    藤田 拓男
    1961 年 37 巻 1 号 p. 20-26,2
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
    Benzoic acid adsorption was used to extract parathyroid hormone from human urine. Bioassay was carried out using the rate of P32 excretion in parathyroidectomized rats. Between the dose level of 5 to 20 USP units, the precision of the assay was satisfactory as indicated by the degree of precision, between 0.2 and 0.3.
    In 5 normal subjects the amount of parathyroid hormone in 24-hour urine was 18.4±3.2 units. In 8 cases of hyperthyroidism before treatment, 61.1±6.9 was found, the difference being significant. (P>0.01) Elevated values were also observed in chronic nephritis, hypertension, nephrolithiasis and essential hypoproteinemia. In postoperative hypoparathyroidism with manifestations of tetany, parathyroid hormone was undectable by the present method.
  • 第2報 特に肺結核患者の喀血時の副腎皮質機能について
    粟井 正則
    1961 年 37 巻 1 号 p. 27-30,2
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
    In order to clarify the relation between stress and hemoptysis, the author measured the urinary excretion of 17-OHCS in pulmonary tuberculosis 35 patients, who had the tendency to hemoptysis (the “far advanced” category and the more serious of the “moderately advanced” of the A. N. T. A. classification), and compared the values with their normal values in the quiescent period.
    While the average daily excretion of 17-OHCS was found to be 4.2 ± 0.3 mg in normal subjects, it was 2.3 ± 0.4 mg in these patients. The figure for those of the patients who actually showed hemoptysis was 2.2 ± 0.3 mg in the quiescent period and the average of the highest values observed during the period of hemoptysis was 3.7 ± 0.6 mg. The excretion in the three days immediately preceding the onset of hemoptysis could be observed in nine patients, of whom one was observed twice. These values were distinctly higher, in all the cases except two than the average of three measurements made during the quiescent period, which was 2.1 ± 0.4 mg. The average of the highest pre-hemoptysis values was 3.9 ± 1.2 mg.
    The differences between the figures are statistically significant excepting the difference between the values before and during hemoptysis.
    The fact that these patients have higher urinary 17-OHCS values a few days before the onset of hemoptysis seems to indicate that some unidentified factor appears in the body and acts as a stress, causing increased excretion of 17-OHCS on the one hand and the onset of hemoptysis on the other.
  • 置塩 郁三
    1961 年 37 巻 1 号 p. 31-49,3
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
    Investigating the basic factor of bioassay utilizing the P32 uptake of one-day-old chick thyroids, we found that the uptake of P32 by thyroid, reached a maximal level in 3 hours, not only in the untreated chicks but also in the chicks which had been subcutaneously injected with TSH. In the case of subcutaneous injections of TSH, the maximal level was obtained between 12-18 hours after injection, and in the intracardially injected with TSH, the response reached the maximal level at around 6 hours after TSH administration.
    By comparing Lambergs' subcutaneous and Greenspan's intracardiac injections of TSH for its bioassay utilizing the P32, we recognized that the Greenspan's method is more appropriate than the Lamberg's with regard to the minimal detectable dose, which was 0.01 J.S.u. in the former while 0.1 J.S.u. in the latter, and the precision index also indicated the former's superiority, that is, 0.31 in Greenspan's and 0.64 in Lamberg's method.
    As to the extraction method of TSH from blood, there is a classical Fellinger's procedure. Using Fellinger's method and Greenspan's intracardiac method, the recovery test was made and showed 24-29% of recovery rate. Substituting the method of centrifugation for filtration, in order to separate the precipitate produced with addition of diluted acetone, the author obtained a satisfactory recovery rate of 53-58%.
    The TSH activity of each fraction was exemined : about 18% of the added TSH was transfered to the first precipitate and 0.5% of it was contained in the second supernant. Therefore it was apparent that 30% undetectably disappeared during the course of the procedure.
    The TSH activities of the blood were examined by the procedure described.
    The chemical analysis of TSH was then carried out by papergraphy, and the author found that the Amidoblack 10 B was most satisfactory for the staining and that the best result was obtained when TSH was dissolved in physiological saline. The author also found that at least 4 components of Ninhydrin positive were separated when the filter paper was developed with the solvent butanol-glacial acetic acid-water.
    Furthermore the author recognized the glucosamine as a suger component of TSH.
  • 青山 善助
    1961 年 37 巻 1 号 p. 50-66_1,4
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
    Comparative studies on the rats were undertaken by administering the thyroid gland powder to successive generations. These rats were offsprings of F1 ; 25 cases, F2 ; 19 cases, F3 ; 21 cases, F4 ; 25 cases cases and F5 ; 48 cases respectively. Estimations were made of body weight, histological examinations, tissue measurements of the thyroid and pituitary glands plasma PBI, total blood cholesterol and I131 uptake of the thyroid gland. There Comparative studies were made with 25 control rats.
    The results are summarized as follows :
    1. The growth weight of offsprings of F4 and F5 male groups was show as compared with the control.
    2. In the thyroid glands of offsprings, the histological findings showed a smaller follicular size, reduced collagen quantity, increased eosinophilic staining with marginal vacuolization, and an increased (length) of follicular cells.
    As to follicular formations, the number of cells tended to be less. However, the cellular area of follicles and the length showed a tendency as to increase generations followed.
    3. In the pituitary body, these offsprings showed basophilia and eosinophilia but with basophilic swelling.
    4. Serum PBI showed an increasing trend with successive generations.
    5. Total blood cholesterol did not show any definite change in the offsprings.
    6. Thyroid uptake of I131 of one and 24 hour values were increased as compared with the controls in both F1 and F2.
    The above findings seem to indicate the increasing hyperfunction of the thyroid when the extract was administered on rat offsprings for successive generations.
  • 1961 年 37 巻 1 号 p. 67-89
    発行日: 1961/04/20
    公開日: 2012/09/24
    ジャーナル フリー
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