日本内分泌学会雑誌
Online ISSN : 2186-506X
Print ISSN : 0029-0661
ISSN-L : 0029-0661
41 巻, 6 号
選択された号の論文の15件中1~15を表示しています
  • Kohachiro KOGA, Yasushi OKAMURA, Yasumasa NOTOMI
    1965 年 41 巻 6 号 p. 677
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    3 women who suffered from consistently anovulatory cycle were treated with indirect electric stimulation of diencephalon.
    Stimulation consisted of the following factors : direct current 3 mA, 3 min and 2mal per week.
    By means of the electro-stimulation therapy which in this instance was used in patients with consistently anovulatory cycle, the authors were able to achieve a definite improvement in all of 3 cases.
  • Mitsunori MURATA
    1965 年 41 巻 6 号 p. 678-679
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    The in vitro resin uptake of radioactive corticosteroids from serum has been studied to establish a simple method for the determination of cortisol in serum (or plasma).
    1) On the in vitro resin uptake of 14C-4-Cortisol (obtained from The Radiochemical Centre in England) from serum : Two ml of sample serum was pipetted to a small test tube containing a known amount of 14C-4-Cortisol. After vigorous shaking, 1 ml of it was pipetted to another test tube containing 600 mg of Amberlite IRA 400 formate. Then, it was placed in a refrigerator for 90 min at 4°C, shaking it every 10 min. The radioactivity was counted in a liquid scintillation spectrometer. The resin uptake of 14C-4-Cortisol was calculated by the follwing formula :
    1-the radioactivity of the supernatant/the initial radioactivity×100.
    The results indicate that the resin uptake of 14C-4-Cortisol is consistent with the change of CBG (Corticosteroids-Binding Globulin) rather than cortisol level in the sample serum.
    2) On the in vitro resin uptake of 3H-Prednisolone (obtained from The Radiochemical Centre in England) from serum : The procedure was the same as that of 14C-4-Cortisol except for the use of Amberlite CG 400 Type 1 instead of Amberlite IRA 400 formate. The results indicate that in vitro resin uptake of 3H-Prednisolane well reflects the cortisol levels in sera.
    3) On the method for the determination of cortisol in serum (or plasma) by using the in vitro resin uptake of 3H-Prednisolone : The standard curve was determined by adding known amounts of nonradioactive cortisol to the standard serum with the same procedure as for the determination of cortisol in the sample serum (as described below).
    Two ml of 95% ethanol was added to 2 ml of sample serum (or plasma) in a small test tube, mixed by shaking and centrifuged for 10 min at 3000 rpm. Two 1 mls of supernatant were evaporated to dryness in similar test tubes with a stream of N2 gas. One ml of standard serum was added to each, and the tubes were incubated for 30 min at 4°C. Then one half ml of 3H-Prednisolone solution was added to each, and the tubes were incubated again for 30 min at 4°C. Two hundreds mg of Amberlite GC 400 Tyre 1 was added to each tube. After that, the procedure was the same as that of 14C-4-Cortisol resin uptake described above. The amount of cortisol in the supernatant was calculated from the standard curve according to the resin uptake and multiplied by 100/25.6 x 50 to express it in pg cortisol per 100 ml of serum (or plasma). The figure of 25.6 means the recovery of cortisol from the supernatant.
    4) The variations of cortisol levels in sera of 4 normal subjects after the administration of ACTH were determined by the present method. On the limited evidence it would appear that a good correlation is obtained with the cortisol level expected. Further investigations of the clinical applicability of this method are in progress.
  • Masahiro YAMAMOTO
    1965 年 41 巻 6 号 p. 680-681
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    The present studies were undertaken to investigate the mechanism (s) by which an anabolic steroid effects the serum cholesterol level in contrast to glucocorticoid action. 2-Hydroxymethylene-17α-methyl-dihydrotestosterone (HMD) were chosen as an example of anabolic steroids and cortisone acetate as that of glucocorticoids.
  • Kozo YAMADA, Tadataka KOIDE, Katumi AOYAMA, Tutomu KUNO, Kazuhiro WATA ...
    1965 年 41 巻 6 号 p. 682
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    The relationship of plasma Insulin-like activity (I.L.A.) with liver damage in diabetics was studied in this report. The plasma I.L.A. was measured by in vivo-assay using hypohysectomized-adrenectomized-rats. The following results were obtained.
    1) The diabetic who was diagnosed with complicated fatty liver by liver biopsy, had higher I.L.A. and the diabetic who was diagnosed complicating liver fibroses or cirrhosis, had lower I.L.A.There seems to be a disturbance of insulin utilization in the former and insulin deficiency in the latter.
    2) Concerning the relation of plasma hepatic enzyme activity in diabetics, the activities of GOT, GPT, and G6Pase in diabetics having lower ILA, were lower than that in diabetics having higher I.L.A. No apparent change was observed in Alkalin phosphatase activity of both groups.
    3) As for plasma I.L.A. and prognosis of liver function, liver function was improved by the treatment for Diabetes, more in cases of diabetics having high plasma I.L.A. than in cases of diabestics having low plasma I.L.A.
    These findings indicate that there is an intimate relationship between plasma I.L.A. and liver damage in diabetics, and suggest that the deficiency of insulin may play directly or indirectly an important role on the complication of liver damage in diabetics.
  • 1. Studies on the fractionation of urinary 17-ketogenic steroids (17-KGS) (using periodate oxidation) and some fundamental aspects of the Metopirone test in children
    Yoshio IGARASHI
    1965 年 41 巻 6 号 p. 683-684
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    A modified Few's method of the urinary 17-KGS determination, by which urinary direct 17-KGS was fractionated into 2 parts of 11-deoxy-KGS and 11-oxy-17-KGS, was applied in examining the pituitary ACTH secretion capability in children in whom adrenocortical 11-fl-hydroxylase inhibitor (Metopirone, SU-4885 CIBA) was administered. Several fundamental coditions were studied with the following results.
    Metopirone capsules, 250 to 500mg a dose, were administered 6-8 times for one day to a daily dosage of 2g. in children with body weight of 20kg or less, and 3g. in those over 20kg.
    1. Changes in urinary 11-deoxy and 11-oxy 17-KGS, 17-OHCS (Porter-Silber Chromogen PSC) and pregnanetriol before and after administration of Metopirone were studied ; both 11-deoxy and 11-oxy 17-KGS seemed to show changes in level of 11-desoxy-cortisol and cortisol secretion. (Fig. 2 and 3).
    2. Increase in urinary 17-KGS and 17-OHCS (by Reddy, Jenkins and Torn's methods) after administration of Metopirone in 25 children of various conditions were compared. The author belives that the increase in 17-KGS excretion is a better index in evaluting the response to Metopirone than 17-OHCS which often showed a low increase and would thus give a false negative interpretation (Fig. 4, Table 1).
    3. Calculating the 17-KGS increment per unit body suface after administration of Metopirone in 10 cases of normal children of 3 to 14 years of age, it was noted that no differences were seen in relation to the age, and 11-deoxy was 5.57-20.87 mg/day/m2 while the total (11-deoxy +11-oxy) was 4.49-21.44 mg/day m2 ; 11-deoxy/11-oxy ratio increased to the maximum of 3.4EL-1.10, Mean (M) -±Standard Deviation (SD). Using this level as the standard, the responses were classified as normal, hyporeactive (limited pituitary reserve, frank insufficiency) and hyper-reactive. Urinary total 17-KS was also determined at the same time ; the increase were less than 1 mg/day ? m2 in chidren under 10 years of age, and the author thinks that this level cannot be used as a criteria in evaluating the response (Fig. 5, 6 and Table 2, 3).
    4. As for the Metopirone administration method, its effect by divided doses and development of side effects were studied. Doses of 250 to 500mg for 6 to 12 times for one day were suitable in children. Administration of 90 to 140 mg/day/kg body weight was necessary in order to maintain the 11-deoxy/11-oxy ratio children of various diseases over the M-1.SD of the normal children, and the admistration method which satisfies both is considered to be suitable in younger children (Fig. 7 and 8).
    5. A case which showed dissociation between the elevation of 11-deoxy-17-KGS and total 17-KGS after the administration of Metopirone is desribed. Differences in the ratio of 11-deoxy/11-oxy was noted in cases which showed inhibition of response to pituitary irraadiation and thyroid treatment (Fig. 9, 10, and 11).
    6. Literature on basic conditions of Metopirone test in children is reviewed on the abovementioned various points (Table 4).
  • Shigemi ASAZUMA
    1965 年 41 巻 6 号 p. 685-686
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    Under the influence of corticosteroids, it is well known that lymphnode falls into marked atrophy. Akasu and Tateno (1963) observed experimentaly the rat lymphnodes, pretreated with cortisone, seldom attacked with the metastasis of rat ascites hepatoma, and they assumed the possibility of arrest of cancer metastasis in man.
    In previous study it has been found in rat lymphnodes that the decrease of DNA contents and the rise of the ratio of RNA to DNA contents were caused by treatment with cortisone. The purpose of the present study is to investigate the changes, induced by cortisone, which probably had prevented the lymphnodes from the metastasis of implanted ascites hepatoma, by means of determination of the nucleic acid contents in the lymphoid tissues.
    Forty female rats, weighing between 80 and 120g., were divided in half. Each animal of the one half received 2.5mg. of cortisone acetate, injected subcutaneously, each day for 10 days (Treated group). Each of the other half received 0.1m1. of physiological salt solution, instead of cortisone, injected similarly (Control group).
    Twenty animals in all, ten animal apiece picked out of the two groups, got ascites hepatoma AH 130 implated intraperitoneally on the seventh day after the first injection of cortisone or salt solution. Then, twenty-four hours after the final injection, all animals were killed. Twenty microcurie of 32P solution was subcutaneouly injected into each animal at six hours before the sacrifice.
    After the sacrifice of the animals, thymus, lymphnodes and spleen were excised for the measurement of the nucleic acid contents and the 32P amounts incorporated into the nucleic acids. Throughout this experiment, ribonucleic acids (RNA) were determined by the color reaction of its pentose with orcine and deoxyribonucleic acids (DNA) by the reaction with diphenylamine, after the separation of RNA and DNA by the method based on Schmidt and Thannhauser procedure.
    The results of the experiment are summarized as follows
    (1) The implantation of ascites hepatoma carried the thymus into atrophy, in which RNA and DNA contents were decreased and the incorporation of 32P into the nucleic acids were remarkably inhibited. These changes occurred in the thymus were promoted by the simultaneous treatment with cortisone.
    (2) Under the influence of the implanted tumor, untreated lymphnodes swelled in size, attended by an increase of RNA contents and a lowering of the ratio of RNA to DNA contents. In treated lymphnodes, however, these changes except for a little hypertrophy were not caused by the implantation of the tumor.
    Reversely, under the influence of the implantation, the increase of DNA contents and the elevation of the ratio of RNA to DNA contents were found in treated lymphodes. This elevation of ratio, induced by cortisone, seems to have some relation to the prevention of the metastasis.
    (3) The incorporation of 32P into the spleen RNA was promoted by the implantation of the tumor unrelated to the treatment. In this experiment, however, neither the treatment with cortisone nor the implantation of the tumor affected the nucleic acid contents of the spleen.
  • 1965 年 41 巻 6 号 p. 687-723
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
  • 特にEstrogenとの関連において
    岡村 靖, 田中 祥照
    1965 年 41 巻 6 号 p. 724-726,676
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    近年, 子宮頸癌の発生過程として, 扁平上皮化生 (squamous metaplasia), 基底細胞増生 (basal cell hyperplasia), 予備細胞増生 (reserve cell hyperplasia), および, 腺腫様増生 (adenomatous hyperplasia) が重視されて来た.しかし, 最近, Harvard大学のYoungeらは, 次いで, Friedellは “pricklecellhyperplasia” から浸潤癌が生じると報じている.すなわち, normal cell⇔prickle cell hyperplasia⇔prickle cell hyperplasia with parakcratosis or keratosis⇔prickle cell hyperplasia with anaplasia either with orwithout keratinization→上皮内癌→浸潤癌の過程が存在すると考えている.しかし, 彼らの示す組織像はprickle cell hyperplasia with anaplasiaを表わしてはいるが, この異形成性上皮変化が果して癌に移行するか否かは即断し難い.
    今井によると, 前癌状態という言葉は, “正確な発癌過程の路線上における必現にして, 且つ, 癌化が約定された, 特定の不可逆状態” に対して用いるべきであるとしている.しかしこの定義を満たす状態を病理形態学的に確認することは仲々困難である.けだし, この経過を動的に把えることが不可能だからである.けれども, ある細胞層から明らかな発癌の進展を示す組織像を認めることが出来たならば, その細胞層からの発癌を示唆すると云える.著者らは, ddN系マウスの子宮頸部に3・4-bcnzpyrcncを塗布し, 同時に種々のホルモンの投与を行ない, 子宮頸癌発生に各種ホルモンがいかなる作用を有するか検索を重ねているが, estradiol投与により, squamo-columnar junction (以降S-GJと略す) に近い扁平上皮領域に著明なprickle cell hyperplasiaを認め, また, prickle cell hyperplasiaからの発癌を示唆する所見を得たので報告する.
  • 古賀 康八郎, 岡村 靖, 納富 廉正
    1965 年 41 巻 6 号 p. 727-730
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    従来,間脳-下垂体障害による無排卵症に対する治療法としては, 臨床上, exogenにHormoneを投与する (主としてGonadotropinが用いられているが, 近年EstrogenおよびCortisonによる排卵誘発も試みられている) か, または, 間脳レ線照射が用いられて来た.しかし, 前者はHormone投与による抗体産生, Gonadotropin大量投与による卵巣腫大, 更に, 稀には茎捻転が認められ, 後者においては照射部位の脱毛および装置の簡便性に乏しい欠点がある.
    近年, 下垂体一卵巣系の上位調節機構として, 大脳皮質→間脳→下垂体の刺戟伝達のメカニズムについての研究に多くの進展をみ, 自律中枢としての視床下部が内分泌系の調節に密接な関連を有することが次第に明らかにされつつあるが, 必然的に治療法としても間脳の機能調整が重視さるべきであり, 私たちは, 動物実験で間脳を直接電気刺激すると排卵が起こる現象 (Hαywqrdら, 1964) に着眼し, 新らたに人の間脳に弱い電流を通じる (頭蓋の上から間接的に) ことによつて, 間脳にmildな刺激を与え, 排卵誘発に成功したので報告する.
  • 村田 光範
    1965 年 41 巻 6 号 p. 731-739
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
  • 山本 昌弘
    1965 年 41 巻 6 号 p. 740-750
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
  • 山田 弘三, 小出 忠孝, 青山 克己, 久野 勉, 渡辺 一弘, 前田 敏夫, 宮脇 洋隆
    1965 年 41 巻 6 号 p. 751-755
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    肝臓が生体の物質代謝に於て果す重要な役割から, 糖尿病時に肝臓の占める意義が極めて大きい事は当然である.古くV. Mering. Minkofskiで膵摘動物の肝臓に多量の脂肪沈着を認めて以来, 糖尿病に於ける肝臓の態度に対し, 種々論議されてきたが, 糖尿病者の肝障害が, 非糖尿病者との間に差はないとするもの, 或いは極めて高率に見出されるものなど, その成績は必ずしも一致していない.近年肝生検, 及び酵素化学の進歩による肝診断法の発展に伴い糖尿病に合併した肝障害は, 改めて注目せられるに至つている.糖尿病がInsulinの絶対的或いは比較的欠乏に基因する代謝異常疾患とされている事から, このInsulin欠乏が, 糖尿病時の肝障害に対して, 如何なる影響を与えるかには極めて重要な問題であるが, 内外の文献に何ら明確な根拠はみられない.私共は依然より独自のInsulin測定法により血中Insulin活性 (以下ILAと略す) を測定し, 糖尿病の病態との関連について検索してきたが, 今回は糖尿病時の肝障害とILAとの関係について報告する.
  • 第1編 過ヨード酸ナトリウム酸化による尿中17 -Ketogenic Steroid (17-KGS) 分画測定の応用と, 小児Metopirone試験に於ける2, 3の基礎条件の検討
    五十嵐 良雄
    1965 年 41 巻 6 号 p. 756-775
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
  • 浅妻 茂美
    1965 年 41 巻 6 号 p. 776-790
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
  • 1965 年 41 巻 6 号 p. 791-795
    発行日: 1965/09/20
    公開日: 2012/09/24
    ジャーナル フリー
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