巻号一覧
前身誌
51 巻, 2 号
選択された号の論文の6件中1～6を表示しています
- |<
- <
- 1
- >
- >|
-
福井 巌, 久城 英人, 八谷 孝, 越智 幸男1975 年 51 巻 2 号 p. 69-75
発行日: 1975/02/20
公開日: 2012/09/24
ジャーナル フリーThe determination method of c-AMP in urine by competitive protein binding assay using the Boehringer Mannheim Laboratory kit was investigated and the results obtained were as follows.
1) Since the angle of the inclination of the standard curve was large, the prseent method could be used for the determination of c-AMP in concentrations of from 0 to 20p moles.
2) The optimal condition of the binding reaction was for 100 minutes of reaction time at pH 4.0 and 4°C.
3) The specificities of binding protein to the other nucleotides were 0 to adenosine, 0.4 to AMP, 0.3 to ADP, 0.4 to ATP and 0.6 to c-GMP respectively when the specificity to c-AMP was chosen as 100.
4) The optimal volume of cold phosphate buffer solution needed for washing to separate binding c-AMP by the filtration method using a millipore filter was 5-15 ml.
5) The precision of the present method by double determination was 0-±11.1% with average of ±5.6% in c.v.
6) The recovery rate of the added c-AMP by the present method was 78.6-105.6 % with average of 90.1 %.
7) Correlation between the determination values of c-AMP of the same samples with the present method and radioimmunosasay (Schwarzman Laboratory Kit) was satisfactorily high with 0.890 in coefficient of correlation, and the determination values by the present method were significantly higher (p<0.05) than those by radioimmunoassay.
8) C-AMP in urine was stable for at least one month when the urine was kept frozen.
It is conclusively considered from the above results that this competitive protein binding assay is a method to determine c-AMP in urine with excellent accuracy and sensitivity and that this method is useful for clinical test.抄録全体を表示PDF形式でダウンロード (686K) -
山本 智英, 土井 啓, 熊原 雄一1975 年 51 巻 2 号 p. 76-84
発行日: 1975/02/20
公開日: 2012/09/24
ジャーナル フリーSimultaneous measurements of serum free thyroxine (T4) and triiodothyronine (T3) fractions were studied using a modification of equilibrium dialysis described by Sterling and Brenner. To 1.2 ml of the serum to be assayed, 131I-T4 and 125I-T3 were added in a concentration of 2 μg/dl and 25 ng/dl, respectively, both of which were preliminarily dialysed according to Schussler and Plager. Half ml of the serum with tracers added was dialysed against 9 ml of phosphate buffer (ionic strength 0.15, pH 7.4) for 18 hours at 37°C and 0.1 ml was reserved from the rest in a counting tube (in duplicate). After the completion of dialysis, the dialysate was mixed with 1 ml of pool serum and the contaminating inorganic iodide (in the form of 131I or 125I) was eliminated by adsorption on anion exchange resin.
The radioactivity of 3 ml of the dialysate and 0.1 ml of the preserved serum (with the tracers added) was counted and the free (or dialysable) fractions were expressed as a ratio of the count of the former divided by that of the latter adjusted to an equal volume by calculation. The amount of either T4 or T3 added as tracers had no influence on free T4 or T3 fraction unless either of them was added to a concentration of 10 μg/dl.
When 125I-T3 of low specific activity (50 μCi/ug) was used as a tracer, free T3 fraction measured simultaneously with free T4 fraction tended to be higher than that measured with a single tracer. When 125I-T3 of higher specific activity (300 μCi/μg) was employed, free T3 fraction obtained with two methods did not differ significantly. Using serum T4 and T3 concentrations measured by competitive protein binding analysis and radioimmunoassay, respectively, the free T4 and T3 concentrations were estimated with sera of normal, hyperthyroid, hypothyroid and uncomplicated pregnant subjects.抄録全体を表示PDF形式でダウンロード (876K) -
第3篇 不顕性甲状腺機能低下状態 (橋本病およびバセドウ病131I療法遠隔例) におけるホルモン分泌動態に関する研究竹田 洋祐1975 年 51 巻 2 号 p. 85-97
発行日: 1975/02/20
公開日: 2012/09/24
ジャーナル フリーChanges in thyroid hormone levels and their relationship with TSH in cases with Hashimoto's thyroiditis and 131I treated Graves' disease in subclinical hypothyroid state were investigated :
1)In 34 cases with Hashimoto's thyroiditis, TSH level was 14.7±26.8 μU/ml (mean±s.d.), T3 144.5±33.6 ng/100 ml; T4 6.7±2.4 μg/100 ml; T3RU 25.4±3.2%; F.T.I. 2.09±0.89; T3/T4 2.46±1.04%.
It was noted that TSH levels revealed significant positive correlation with T3/T4 (r=0.77); significant inverse correlation with F.T.I. (r=-51); and non-significant inverse correlation with T3 levels (r=-0.06).
These results indicate that T3 might play a more important role than T4 in maintaining normal thyroid function (so-called T3 euthyroidism). This is probably based on metabolic disturbance of iodine which is common in Hashimoto's thyroid.
In several cases with rather high normal basal T3 levels, no significant elevation in T3 was observed in spite of elevated TSH level following TRH administration. This might indicate that the thyroid is fully functioning and that no more reserve is left for the thyroid to respond further to elevated TSH.
2) In 30 cases with 131I treated Graves' disease, TSH concentration was 22.3±29.8 μU/ml (mean±s.d.); T3 100.7±35.4 ng/100 ml; T4 6.2±2.9 μg/100 ml; T3RU 25.6±2.3%; F.T.I. 1.87±0.85; T3/T4 1.80±0.64.
Significant inverse correlation was noted between TSH levels and F.T.I. as well as T3 levels. Although TSH levels showed significant positive correlation with T3/T4 (r=0.44), its degree of correlation was not as good as that with F.T.I. (r=-0.71) and T3 (r=-0.53).
These results indicate that there is no preference in T3 production. From the responsiveness of thyroid and pituitary following TRH administration, assumption can be drawn that 131I treated Graves' patients, who become euthyroid, may in fact be on the way to hypothyroidism.抄録全体を表示PDF形式でダウンロード (1288K) -
長岡 研五, 桜美 武彦, 深瀬 政市, 小川 隆三1975 年 51 巻 2 号 p. 98-102
発行日: 1975/02/20
公開日: 2012/09/24
ジャーナル フリーIn the course of studies on the occurrence of antithyroid antibodies in various thyroid disorders, serum antibodies to microsome of thyroid epithelial cells, as well as circulating antibodies to thyroglobulin, are demonstrated by tanned red cell hemaggulutination.
These thyroglobulin and microsome-coated tanned red blood cells can be efficiently demonstrated with a commercially prepared reagent. (Fuji-Zoki Co.)
The sera of 2,350 normal subjects were tested by these thyroid autoantibodies tests.
Fifty-one (2.3 per cent) of the sera of 2,350 normal subjects showed a positive reaction for thyroglobulin antibodies, and fifty-nine (2.5 per cent) persons showed a positive reaction for microsomal antibodies.
The incidence of thyroglobulin and microsomal antibodies in males and females were progressively greater with age, particularly between ages 60 to 69.抄録全体を表示PDF形式でダウンロード (546K) -
栗林 忠信, 湯地 重壬, 矢島 義忠1975 年 51 巻 2 号 p. 103-108
発行日: 1975/02/20
公開日: 2012/09/24
ジャーナル フリーThe relation of the TRH test to the T3 suppression test was investigated in 43 patients with hyperthyroidism reteiving antithyroid drugs for 6 to 27 months (average 14 months). TRH tests were performed by measuring serum TSH levels before and 15, 30, 45, 60, 90 and 120 minutes after intravenous injection of 500 μg of synthetic TRH. Serum TSH was measured by a double antibody radioimmunoassay. Two weeks later, the T3 suppression test was performed by measuring the 24-hr thyroidal uptake of radioiodine after daily administrations of 75 μg of triiodothyronine for 8 days. All patients under study were in euthyroid state, estimated by serum T3-RSU, T4, T3 and FT4I.
The value for 24-hr uptake after T3 administration was less than 20% in 18 cases, out of which the response to TRH was normal or exaggerated in 15 cases and was absent or impaired in 3 cases. On the other hand, out of 25 cases with the value of 24-hr uptake more than 20%, the response to TRH was absent or impaired in 18 cases and was normal or exaggerated in 7 cases.
The results of the TRH test and the T3 suppression test were correlated in 33 out of 43 cases. The responsiveness to TRH and the suppressibility with T3 was dissociated in 10 cases, suggesting the TRH test could not replace the T3 suppression test.抄録全体を表示PDF形式でダウンロード (725K) -
1975 年 51 巻 2 号 p. 109-128
発行日: 1975/02/20
公開日: 2012/09/24
ジャーナル フリーPDF形式でダウンロード (1597K)
- |<
- <
- 1
- >
- >|