巻号一覧
前身誌
52 巻, 10 号
選択された号の論文の8件中1～8を表示しています
- |<
- <
- 1
- >
- >|
-
-特にACTH, 下垂体摘出及びAminoglutethimideの影響-宮地 幸隆1976 年 52 巻 10 号 p. 973-982
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーThe lipid patterns in the adrenal glands of rats with the adrenocortical steroidogenesis stimulated by ACTH or inhibited by hypophysectomy or by aminoglutethimide (AGT) were compared with those in intact rats. The administration of ACTH caused the gradual decrease in the concentration of cholesteryl ester with the preferential decrease in the proportion of arachidonic acid. The deposition of cholesteryl ester was observed in the adrenal glands of both hypophysectomized and AGT treated rats. Hypophysectomy was accompanied by the increase in the proportion of 16 : 0 and 18 : 0 and the decrease of 18 : 2 and 20 : 3, while AGT administration caused the increase in the proportion of 20 : 4 and the decrease of 22 : 6 in the cholesteryl fraction.
ACTH and AGT treatments or hypophysectomy resulted in minor alterations of the concentrations and fatty acid compositions of triglyceride and phospholipid.
The marked differences in the concentration as well as fatty acid compositions of cholesteryl ester under the stimulated or inhibited adrenal steroidogenesis suggest the important role of fatty acids esterified with cholesterol in the steroid hormone synthesis.抄録全体を表示PDF形式でダウンロード (958K) -
山田 研一, 田村 泰, 山本 昌弘, 熊谷 朗1976 年 52 巻 10 号 p. 983-996
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーPseudohypoparathyroidism (PHP) is a disorder chracterized by renal and/or skeletal refractoriness to the effect of parathyroid hormone (PTH). In 1973, it was first proposed by Drezner et al that PHP could be divided into two types, e.g., PHP Type I and PHP Type II. PHP Type I is a disorder which fails to show the increase of urinary c-AMP and phosphaturia by the administration of PTH, but PHP Type II is that which responds to the administration of PTH with a marked rise of urinary c-AMP, but no increase of phosphate excretion. In 1974 Rodriguez et al demonstrated a patient with PHP Type II who restored normal renal responsiveness to PTH by calcium administration. Here we present two patients who fitted in the categories of PHP Type I & II, respectively, and restored normal renal responsiveness by the combined Ca-PTH administration or dibutyryl-cAMP infusion.
Case I (PHP Type I) was a 31 yr old male with marked hypocalcemia and hyperphosphatemia, who showed neither increase of urinary c-AMP nor phosphate excretion in response to PTH infusion. Case II (PHP Type II), also diagnosed as Sjögren syndrome was a 22 yr old female with relatively mild hypocalcemia and hyperphosphatemia, who showed a marked rise of urinary c-AMP, but no increase of phosphate excretion by PTH administration. Acute infusion of calcium, followed by PTH administration restored renal responsiveness to PTH in both types, though calcium infusion showed only little effects.
The patient of PHP Type I received calcium gluconate and Vitamin D therapy and serum Ca improved, when PTH or DBc-AMP was given with the reappearance of PTH-like action.
Thus in PHP Type I, the lack of c-AMP response to PTH, coupled with the ability of infused DBc-AMP to evoke a normal renal response suggests that the metabolic defect in this disorder may exist in hormone receptor-adenyl cyclase complex. Calcium infusion followed by PTH administration probably might evoke calcium influx and c-AMP production, resulting in the reappearance of hormone action.
In PHP Type II, as c-AMP generation system is intact, the metabolic defect can be thought to exist in the rather poorly defined process beyond c-AMP generation. The combined Ca-PTH administration also restored normal renal response, probably partly due to the improved calcium environment of renal tubular cells.
Though it is difficult to explain why endogenously generated c-AMP had no effect, but DBc-AMP had on reappearance of PTH-like action, it is speculated that DBc-AMP may have much more stronger effects for intracellular receptor site of c-AMP in PHP Type II.
Conclusively these results clearly suggest that the nature of the metabolic defects in PHP is not a genetically determined inrreversible disorder, but rather a functional one which can be reversibly restored by special conditioning.抄録全体を表示PDF形式でダウンロード (1283K) -
宮保 進, 久田 友一郎, 岸田 繁, 安里 公1976 年 52 巻 10 号 p. 997-1007
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーMetabolism of water-soluble synthetic corticosteroid esters, hydrocortisone hemisuccinate (H-H); hydrocortisone phosphate (H-P), prednisolone hemisuccinate (P-H), prednisolone phosphate (P-P), dexamethasone phosphate (D-P) and dexamethasone sulfate (D-S) was studied by i.v. administration of steroids in a dose of 100 mg to 6 healthy volunteers and consequtive urine collections over 24 hrs. Uniary metabolites were separated into free, glucuronide, sulfate and “unhydrolyzed” fractions and measured by means of Porter-Silber (P-S) reaction and isonicotinic acid (INH) reaction.
In H-H, H-P and D-S, glucuronide conjugates constituted the largest fraction of P-S positive metabolites in 24 hr-urine. In contrast, P-H, P-P and D-P were excreted mainly as free metabolites. Small but variable portions were found as sulfate and “unhydrolized” conjugates. Time course study revealed that, in H-H, H-P and D-S, the free/glucuronide ratio of P-S positive metabolites decreased progressively or remained low throughout the collection periods, whereas in P-H, P-P and D-P the ratio increased markedly in the 4-12 hr period. For all steroids, the INH/P-S ratio was found to be high in the free fraction and low in the glucuronide fraction; the tendency was especially marked in P-H, P-P and D-P. INH-positive metabolites were also predominant in the sulfate and “unhydrolyzed” fractions.
The results indicate that the metabolism of synthetic corticosteroids, in general, is characterized by diminised rate of ring A reduction followed by glucuronide conjugation and compensatory increase in free metabolites, the bulk of which consists of ring A intact, C-20 reduced metabolites. 6-Hydroxylated metabolites may also be increased. Conjugation with sulfuric acid occurs but to a small extent. The consistent presence of “unhydrolyzed” metabolites suggests that portions of esters can be excreted unsplitted. It is also noted that the structure of steroid moieties and type of esters were important factors determining the quantitative and qualitative difference in their metabolic fates.抄録全体を表示PDF形式でダウンロード (991K) -
麻生 武志1976 年 52 巻 10 号 p. 1008-1019
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーA radioimmunoassay method has been developed for the simultaneous determination of pregnenolone, pregnenolone sulfate, dehydroepiandrosterone (DHA) and dehydroepi-androsterone sulfate (DHA sulfate). The method consists of the following procedures : 1) ether extraction of unconjugated compounds, 2) extraction of sulfates from aqueous residue with ethyl acetate, 3) solvolysis with sulfuric acid at 40°C for 60 minutes, 4) celite column chromatography to separate individual compounds, 5) radioimmunoassay. Efficiencies of solvolysis for pregnenolone sulfate and DHA sulfate are 94 and 80%. Precision and accuracy studies have shown that the assays of sulfates as well as unconjugates are reproducible and accurate. Specificity was ascertained by parallelism and linearity studies. No interfering substance was detected in appreciable quantity.
Plasma levels of these four compounds were determined in specimens obtained from 15 normally ovulating women. To represent the whole menstrual cycle, samples were taken 8 days before LH peak (LH-8), the day of LH peak (LH=0) and 8 days after LH peak (LH+8). Plasma contents of these compounds (geometric mean in ng/ml and 95% confidence limits in parentheses) are as follows : pregnenolone, LH-8 : 1.33 (1.02-1.74), LH=0 : 1.45 (1.22-1.72), LH+8 : 1.88 (1.70-2.21); pregnenolone sulfate, LH-8 : 70.0 (55.9-89.2), LH=0 : 57.5 (40.0-82.7), LH+8 : 102 (81.5-129); DHA, LH-8 : 5.38 (3.90-7.43), LH=0 : 4.90 (3.58-6.79), LH+8 : 4.58 (3.12-6.83); DHA sulfate, LH-8 : 1480 (1110-1980), LH=0 : 1570 (1150-2140), LH+8 : 1590 (1150-2190). Both pregnenolone and pregnenolone sulfate levels of 8 days after LH peak are significantly higher than those of other two days. Conversely, plasma DHA and DHA sulfate levels fluctuate over wide range with no consistent trend.抄録全体を表示PDF形式でダウンロード (1171K) -
池窪 勝治, 小西 淳二, 中島 言子, 遠藤 啓吾, 鳥塚 莞爾, 森 徹1976 年 52 巻 10 号 p. 1020-1032
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーIn one case of untreated Hashimoto's disease, serum thyroxine (T4) value by radioimmunoassay (RIA) was significantly lower than that by competitive protein binding analysis (CPBA). The discrepancy was found to be due to the presence of antithyroxine autoantibody in the serum. This phenomenon was considered to be of practical importance in interpreting the T4 value by RIA in cases with autoimmune thyroid diseases.
The patient was a 59-year-old woman with a 30-year history of goiter. A diagnosis of Hashimoto's thyroiditis had been established by open biopsy of the thyroid ten years ago. The patient was judged to be euthyroid on the basis of clinical and laboratory evaluation (mean serum T4 by CPBA (Tetrasorb and Tetratab kit), 5.0 μg/ 100 ml; serum T3, 165 ng/100 ml; T3 resin uptake, 31.8%; and serum TSH, 2.0 μU/ml). TBG binding capacity was 24 μg/100 ml. Anti-thyroglobulin antibodies (anti-Tg), once positive ten years before, was negative at this time. But the mean T4 in the serum measured by T4 RIA and RIA-Mat T4 kit were 1.7 and 2.9 μg/ 100 ml, respectively.
Recovery of the T4 added to the patient's serum evaluated by RIA-Mat T4 kit, was 71.2%, although the recovery using a control serum was 108%.
Binding of 125I-T4 to the serum or fractions of the serum was studied by using polyethylene glycol (PEG) method, column chromatography, and double antibody precipitation.
The results were as follows :
1) The binding of 125I-T4 to the patient's serum was detected by using RIA kit system without addition of anti-T4 serum.
2) On Sephadex G-200 chromatography of 125I-T4 incubated with the serum or the rabbit anti-T4 antibody in the presence of ANS, an early radioactive peak was observed by using the patient's serum as in the case of the anti-T4 antibody. When the serum after thermal inactivation of TBG, was incubated with 125I-T4, and was applied to the Sephadex G-200 column, a radioactive peak was observed in the area where 7S fraction was detected by protein peak.
3) The binding of 125I-T4 to the patient's IgG was 9.0% by using double antibody method when the binding to a control IgG was 0.5%.
4) The binding of 125I-T4 to IgG fractions was also proved by PEG method.
5) The binding of 125I-T4 was competitively inhibited by the addition of unlabeled T4. The affinity constant was 1.9 X 108 L/mol and its binding capacity was 0.8 μg/100 ml serum.
From these data this T4 binding IgG was considered to be anti-T4 autoantibody. The cross reaction with T3 was approximately 8.3%. MIT and DIT did not displace labeled T4 when tested in amounts varying from 0.1 to 100 ng/assay. By using the paper electrophoresis, the binding of 125I-T4 to the serum or IgG was not detectable. Therefore this method was considered unsuitable for detecting such anti-T4 antibody.
As we couldn't find any significant binding of 125I-T4 to sera in 37 other patients with Hashimoto's disease by using the PEG method, the incidence of this phenomenon was considered to be low. But the presence of such a case has to be kept in mind to avoid erroneous clinical interpretation of T4 value by RIA assay after extraction should be performed when the data is discordant with the clinical state, especially in cases with autoimmune thyroid diseases.抄録全体を表示PDF形式でダウンロード (1211K) -
中井 利昭, 水口 弘司, 森 宏之, 木川 源則, 坂元 正一1976 年 52 巻 10 号 p. 1033-1039
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーChanges of serum concentrations of LH were measured in fetal and neonatal rats following castration.
Intact fetal and neonatal male rats showed low levels of serum. LH concentration. Though serum LH levels of male rats casterated on the 20th day of gestation did not significantly increase by the 22nd day of fetal age, serum levels of neonatal male rats increased 2-to 3-fold 3 days following castration at all ages studied. The increase was greater in 4-day-old male rats than in 1-day-old males.
The increase of serum LH after castration was also found in 4-day-old male rats castrated on the 20th day of gestation.
In contrast, serum levels of 1-day-old neonatal female rats did not increase 3-days after castration.
These observations suggest sex differences in maturation of the gonadal-hypophyseal feedback mechanism.抄録全体を表示PDF形式でダウンロード (688K) -
新美 仁男, 佐々木 望, 松本 生, 首村 紀夫, 中村 陽子1976 年 52 巻 10 号 p. 1040-1045
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーAn epidemiological survey on the incidence of chronic lymphocytic thyroiditis in childhood was performed in 11,353 apparently healthy school children in Chiba prefecture, Japan.
The present study included 9,416 school children (4,401 boys and 5,015 girls, ages 6-18 yrs) in Chiba City and 1,937 children (744 boys and 1,193 girls, ages 16-18 yrs) in Tateyama City. The first group was selected as a representative of urban area, and the second group was selected as that of seaside area.
Children having goiter were selected for testing antithyroglobulin and antimicrosomal antibodies in sera. Final diagnosis of chronic lymphocytic thyroiditis was based on histological specimens obtained by needle biopsies on the antithyroid antibody positive subjects.
The overall incidence of chronic lymphocytic thyroiditis in these children was 1.7 per 1,000 children. There was a considerable sex difference in the prevalence. None of the patients were boys. In girls the incidence increased with age : ages 6-12 0.9, ages 13-15 4.6 and ages 16-18 3.1-4.2 per 1,000, respectively. The incidence in the seaside area, 2.6 per 1,000 was not significantly higher than that in the urban area, 1.8 per 1,000. Histologically, all cases were classified as focal thyroiditis.抄録全体を表示PDF形式でダウンロード (545K) -
福地 總逸, 中嶋 凱夫1976 年 52 巻 10 号 p. 1046-1052
発行日: 1976/10/20
公開日: 2012/09/24
ジャーナル フリーFour methods; retroperitoneal air insufflation study, adrenal phlebography, adrenal scintiscan and estimation of plasma aldosterone in adrenal or renal veins, were compared to determine the diagnostic value for the location of adrenal adenoma in 27 patients with primary aldosteronism and one with idiophathic aldosteronism.
The location of adrenal adenoma could be certainly determined, in 12 of 27 patients with primary aldosteronism by retroperitoneal air insufflation study, in 7 of 22 by adrenal phlebography, in 18 of 23 by estimation of aldosterone in adrenal or renal vein blood, and in 19 of 27 by adrenal scintiscan.
From these results, it is concluded that firstly adrenal scintiscan with 131I-cholesterol and then adrenal phlebography and estimation of aldosterone in adrenal and renal vein blood combined with retroperitoneal air insufflation study should be performed for determination of location of adrenal adenoma in primary aldosteronism.抄録全体を表示PDF形式でダウンロード (745K)
- |<
- <
- 1
- >
- >|