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53 巻, 2 号
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三好 正規1977 年 53 巻 2 号 p. 93-109
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリーIt is well known that antithyroglobulin antibody is frequently found in patients with collagen diseases. Whether the positive anti-TG antibody test in these patients indicates the presence of subclinical thyroiditis or merely represents the serological overlap phenomenon is not certain. The purpose of present studies is to examine the hypophyseo-thyroid function in various collagen diseases to clarify this point. Anti-TG antibodies were positive in 45.9% of SLE, 61.5% of Sjögren's syndrome, 28.6% of RA, 30.0% of PSS, 14.3% of Behcet's desease. TRH-test was performed on 63 patients with various collagen diseases. Expect for patients who were associated with overt thyroid disease (eg. chronic thyroiditis : 5, subacute thyroiditis : 1, thyroid adenoma : 1), ten patients (SLE : 6, RA : 1, PSS : 1, SjOgren's syndrome : 2) exhibited exaggerated TSH responses to TRH. In five out of these patients (50%), anti-TG antibody was positive and in six patients (60%) antimicrosomal antibody was positive. This may indicate the presence of so-called asymptomatic autoimmune thyroiditis (AAT) in these patients.
In postmortal examination of the thyroid glands in 10 patients with collagen diseases, lymphocytes infiltrations were found in three patients with SLE who showed positive anti-TG antibodies and no evidence of overt thyroid diseases.
Therefore, in these cases AAT was proved histologically. The TRH-test may be helpful to detect the minimal abnormalities of pituitary-thyroid function, especially in patients such as those having asymptomatic autoimmune thyroiditis.抄録全体を表示PDF形式でダウンロード (1726K) -
山口 晃弘1977 年 53 巻 2 号 p. 110-130
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリーWhen performing a parathyroidectomy for primary hyperparathyroidism surgery is the therapy of choice for histological variations such as adenoma, hyperplasia, and carcinoma. Surgical procedures for adenoma, hyperplasia, and carcinoma vary as the histological diagnosis indicates. Since the histological evaluation of parathyroid pathology is so difficult we feel that the evaluation procedure should be done by the surgical table at the time of surgery.
A clinicopathologic study of 36 patients operated on at the 1st Dept. of Surgery, Nagoya University was undertaken. 24 of the patients were thought to have single adenomas. Eleven patients had multiple parathyroid enlargements, and of these two were thought to have multiple adenomas. Adenomas and hyperplasias occurred concurrently in two cases. Of the seven cases thought to be hyperplasia, three had near normal glandular histology.
It appears from this histological study that there are transitional forms in some para-thyroid lesions. It is supposed that once the normal parathyroid gland has developed hyperplasia it can in turn lead to adenoma.
Electron microscopic investigation was also made of the parathyroid glands. Parathyroid tissue was obtained from 22 patients who had undergone parathyroid or thyroid surgery. 13 had single adenomas, two had multiple adenomas, two had mixtures of adenomas and hyperplasias, two had hyperplasias, and three had normal glands.
In the normal parathyroid glands mentioned above, abundant glycogen granules were frequently seen, and the lipid bodies were compactly collected in the cytoplasm. The rough surfaced endoplasmic reticulum was small in number and sparse. The Golgi complex was insignificant. A few secretory granules were randomly seen throughout the cytoplasm.
In the hyperfunctioning glands, lipid bodies of the collected type were hardly seen, but a few lipid bodies of a solitary type were scattered in the cytoplasm. Glycogen granules decreased in amount in most cases. The Golgi complex and secretory granules tended to increase. Especially observed was the remarkable increase in the size and number of the rough surfaced endoplasmic reticulum. Their shape altered to a rounded concentric structure. In several cases of parathyroid adnoma, there was evidence that the rough surefaced endoplasmic reticulum transmuted to annulate lamellae. It was proved that these annulate lamellae originated from the rough surfaced endoplasmic reticulum. Annulate lamellae were found in small adenomas and were not found in large adenomas and hyperplasias. One of these cases showed normal glands microscopically but under the electron microscope hyperfunction became apparent. The case was diagnosed as hyperplasia from ultrastructural findings.
In conclusion; In cases of enlargement of even two parathyroid glands, a three and one-half gland parathyroidectomy should be performed. We feel that this should be done because in cases such as these other glands are mostly hyperplastic. Electron microscopic investigation of the parathyroid glands is helpful to differentiate hyperplasia from normal glands.抄録全体を表示PDF形式でダウンロード (9505K) -
伊藤 光泰, 板津 武晴, 山内 一征, 原 巌, 仁瓶 禮之, 富田 明夫1977 年 53 巻 2 号 p. 131-141
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリーIn order to evaluate the effects of simultaneous administrations of propranolol and glucagon, the changes of plasma human growth hormone (HGH) and other hormones following the oral administration of 40 mg propranolol and the subcutaneous administration of 1 mg glucagon were observed in 9 normal subjects, 8 patients with pituitary dwarfism, 1 with Sheehan's syndrome, 3 with Turner's syndrome, 8 with anorexia nervosa, 1 with Fröhlich's syndrome, 2 with simple obesity, and 1 with hypogonadotropic eunuchoidism. The following results were obtained.
(1) In normal subjects, Turner's syndrome, anorexia nervosa, hypogonadotropic eunuchoidism and simple obesity, the peak levels of plasma HGH in P-G administration were more than 8.3 ng/ml, while much lower peak levels were observed in other tests, insulin tolerance test, arginine tolerance test and L-Dopa test. On the other hand, in patients with pituitary dwarfism, Sheehan's syndrome or Fröhlich's syndrome, the peak levels of plasma HGH in P-G administration were less than 4.0 ng/ml.
(2) In P-G administration, the significant increase of plasma cortisol level was observed in patients with isolated GH deficiency or anorexia nervosa as well as in normal subjects.
(3) The plasma IRG levels increased significantly at 30 to 90 minutes and decreased at 180 minutes after P-G administration, and blood sugar levels increased at 30 to 60 minutes and decreased at 120 to 180 minutes. Plasma FFA revealed the lowest levels at 90 to 120 minutes.
(4) Following the, subcutaneous administration of glucagon, plasma IRG increased significantly at 30 minutes, and maintained the high level even at 180 minutes.
(5) There was no relationship between changes of plasma HGH level and other hormones.
From these data P-G administration seems to be a more potent stimulation test for GH reserve, in comparison with other tests.抄録全体を表示PDF形式でダウンロード (977K) -
伊藤 眞次, 広田 良二1977 年 53 巻 2 号 p. 142-150
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリー出生後18時間以内の新生ラットに, testosterone, DHEA, progesterone, estradiol-17β, estrone, estriol, 2-OH estradiol, 2-OH estriol, deoxyestrone, diethylstilbestrolを皮下注射し, 3日後副腎および胸腺の変化を調べ, つぎの結果を得た.
1) 体重の増加はDHEA, 2-OH estriolおよびdeoxyestroneで抑制された.胸腺重量はprogesteroneおよびdiethylstilbestrol以外の各種のsteroid処置で有意に減少した.脾重量の変化は胸腺のそれより軽度であった.副腎はDHEAおよびprogesterone処置で対照より小さく, estroneおよびdiethylstilbestrol処置で肥大した.
2) 副腎corticosterone含量はestrogen処置で著明に増加し, その効果はestriolでもっとも強かった.estradiolおよびestroneでも大きく増加したが, 2-OH estradiolおよびdeoxyestroneには効果がなく, 2-OH estriolの効果は弱かった.一方, DHEAはcorticosterone含量を著明に減少した.
3) cortisolとestriolを同時に注射したばあい, cortisol単独投与にくらべて胸腺と脾の退行が大となったが, 副腎には差がなかった.あらかじめcortisolで処置し, その後estriolを投与すると, cortisolによる副腎の変化が軽度ながら有意にふせがれた.
以上の成績から, estrogen効果の弱いestriol, androgen効果の弱いDHEAの生理的役割, とくに副腎におよぼす影響について考察を加わえた.抄録全体を表示PDF形式でダウンロード (866K) -
小山 嵩夫, 斉藤 幹, 萩野 信義1977 年 53 巻 2 号 p. 151-158
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリーRecently it has been observed that biphasic (former and later) elevation of peripheral LH levels with increment of plasma estrogen and progestin is induced by LH-RH injection in the baboon, and it is inferred that elevation of circulating estrogen and progestin exerts it's effect on hypothalamus and/or pituitary as to the later LH release. This study was performed to investigate the effect of TRH, following LH-RH injection, on the pituitary-gonadal system in relation to LH release and plasma levels of ovarian hormones. Hundred jig of LH-RH was injected s.c. during the early luteal phase (2 to 5 days after ovulation) in 5 normal menstruating baboons. Blood samples collected every 15 minutes for the first one hour and every 30 minutes for the next 2 hours were assayed for LH, estrogen and progestin. Plasma LH peak appeared within 30 minutes, in addition, another LH release appeared within 90 to 150 minutes after LH-RH injection. Further, plasma levels of estrogen and progestin were elevated within 30 to 45 minutes after injection. In the next early luteal phase of the same 5 baboons, 40 μg of TRH following 100μg of LH-RH was injected s.c. twice at 30 minutes interval and blood samples were collected in the same manner. An increment of plasma progestin and no changes of plasma LH and estrogen were observed after TRH injection as compared to control levels obtained by only LH-RH injection. This result suggests that TRH may be involved in the regulation of luteinization. The following study was therefore performed as a preliminary experiment. In 3 baboons, 40 μg of TRH was administered s.c. every day for 4 days from the day after ovulation and blood samples collected every other day from the last day of TRH injection to menstruation were assayed for progestin. An increment of plasma progestin was observed after TRH injection as compared to the plasma progestin level during a control period in the same subjects. These findings infer that TRH may have a luteotropic action during the early luteal phase of the menstrual cycle in the baboon.抄録全体を表示PDF形式でダウンロード (727K) -
小時田 宏仂, 南沢 俊郎, 菅原 俊郎, 松井 元右, 鎌田 修二, 富沢 信夫, 金沢 重俊, 木村 武1977 年 53 巻 2 号 p. 159-166
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリーPlasma aldosterone level (PAL) and deoxycorticosterone level (PDL) were determined in 5 subjects of normal sodium intake and 5 subjects of low sodium intake after the intramuscular administrations of ACTH (Upjohn, Cortrosyn) 40 IU, using radioligand methods.
PAL and PDL reached their peaks at one hour after the administrations of ACTH, and then decreased in their normal ranges. Pretreated with the oral administrations of Dexamethasone 2 mg/day for 2 days, these subjects had, when ACTH were injected, such a similiar pattern in PAL and PDL as the peak of one hour and the consecutive falling without the pretreating of Dexamethasone.
PAL and PDL of these subjects were not different between normal sodium intake and low sodium intake during the course of the experiment.抄録全体を表示PDF形式でダウンロード (633K) -
日置 長夫, 能登 康夫, 細島 弘行1977 年 53 巻 2 号 p. 167-174
発行日: 1977/02/20
公開日: 2012/09/24
ジャーナル フリーThe first successful analysis of estrogens in serum and urine was performed by mass fragmentography using a GC-MS combined system. The equipment used was Shimadzu LKB 9000 GC-MS (MID-PM). The TMSi derivative of the compounds were analyzed using the GC-MS system equipped with a 3 ft x 3mm column packed with 1.5% OV-1 and the temperature was programed from 200° to 260°C at 10°C/min increments. The mass spectrum showed molecular ions at m/e 342,416 and 504 which correspond to the TMSi derivatives of Estrone (E-1), Estradiol (E-2) and Estriol (E-3) respectively. Molecular ion peak of each compound was applied to establish the precise quantitative evaluation of E-1, E-2 and E-3.
1) The minimum detectable limits of the compounds injected into the column were ca. 2 pg for E-1, E-2 and 5 pg for E-3 respectively.
2) The sensitivity was of the order of ng or pg which enables quantitation with 2 ml of human serum and urine samples obtained from normally ovulating women.
3) This method was very convenient for extracting the compounds from biological fluids, and the procedure can be carried out easily in a short time.
4) Mass fragmentography makes possible the simultaneous measurements of E-1, E-2 and E-3 in samples obtained from pregnant women.抄録全体を表示PDF形式でダウンロード (2270K)
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