巻号一覧
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57 巻, 10 号
選択された号の論文の8件中1～8を表示しています
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PG産生阻害剤による幼若ブタ過排卵抑制時の黄体河合 康夫, 佐藤 和雄, 三橋 直樹, 荷見 勝彦, 榊原 賢一郎, 木下 勝之, 呉 道明, 坂元 正一, 山田 豊1981 年 57 巻 10 号 p. 1475-1488
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーThe inhibitors of prostaglandin (PG) production (indomethacin, etc.) block ovulation. But little is known about how the inhibition of PG production effects cell function in the follicle. This study was done to clarify the morphological changes and steroidogenesis in the follicles treated with the inhibitor. Fifteen gilts treated with PMS and hCG were administered with indomethacin of 7.6 mg/kg (enough for a complete block) 24 hrs. after hCG. The morphological study of the follicles was done by serial section. The levels of estradiol 17β, testosterone, 17αOH progesterone and progesterone were estimated by RIA in the follicular fluid, ovarian vein and peripheral blood.
A histologic section of the treated follicles revealed the presence of entrapped oocytes surrounded by heavily luteinized granulosa-theca cells. No morphological changes were found in the luteal cells at any stage of the luteal phase except for retention of fluid in the central cavity of the corpus luteum. The life span of the corpus luteum (18 days) was not affected by indomethacin. No differences in steroid levels were detected in the peripheral and ovarian vein blood or in the follicular fluid at any time compared with the control.
Inhibition of PG production results in the luteinized unruptured follicle without interference with steroidogenesis.抄録全体を表示PDF形式でダウンロード (2274K) -
鴨井 久司1981 年 57 巻 10 号 p. 1489-1495
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーA relationship between blood pressure and the functional character of osmoreceptor controlling vasopressin secretion under usual physiological situations in the rat was studied by employing Na-nitroprusside as depressor agent or norepinephrine as pressor agent and the sensitive and specific radioimmunoassay of arginine vasopressin (AVP).
Na-nitroprusside or control saline was given s.c. 15 min after an i.p. injection of 2% (v/w) hyper, hypo or isotonic saline. Norepinephrine or control saline was given s.c. with a concurrent i.p. injection of 2% (v/w) hyper, hypo or isotonic saline. 30 min after this osmotic load, plasma AVP (pAVP) and osmolality (pOSM) were measured on trunk blood as previously described (J. Clin. Invest. 52 : 3212, 1973). In rats given Na-nitroprusside, 2mg/kg body weight, osmotic loading produced a rise in pAVP which correlated relationship (pAVP =1.9 [pOSM-276.6]; N=16; r=0.81) raised significantly (p<0.001) to the left of that in the control vehicle (pAVP=1.4 [pOSM-293.8]; N=8; r=0.86). In rats given norepinephrine, 0.1 mg/kg body weight, the regression line generated by osmotic loading (pAVP=1.2 [pOSM229]; N=7; r=0.88) fell significantly (p<0.001) to the right of that in the control vehicle (pAVP=1.4 [pOSM-292]; N=6, r=0.99). In 4 rats, the same dose of Na-nitroprusside and norepinephrine resulted in a significant (p<0.001) decrease (40%) and increase (20%) from the basal level for the same time of osmotic loading in mean arterial blood pressure as measured directly via chronically implanted aorta catheters, respectively. These results indicate that hypotension increases pAVP by lowering the threshold or set and increasing the sensitivity of the osmoregulatory system and hypertension decreases pAVP by raising the threshold or set and reducing the sensitivity of the mechanism without abolishing the system.抄録全体を表示PDF形式でダウンロード (647K) -
春山 和見, 山崎 正明, 土岐 高久, 福地 総逸1981 年 57 巻 10 号 p. 1496-1507
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーSecretion and metabolism of aldosterone were studied in 7 patients with hyperthyroid-ism before and after treatment.
After the restriciton of dietary sodium to 153 mEq per day over a period of at least 7 days, triiodothyronine (T3), thyroxine (T4), plasma renin activity (PRA) and plasma aldosterone concentration (PAC) were measured before and after the intravenous administration of furosemide (1 mg/kg) in an upright position for two hours. T3, T4, PRA, PAC, aldosterone secretion rate (ASR) and circulating plasma volume (CPV) were assayed repeatedly after the improvement of the symptoms. The disappearance rate of 1, 2, 6, 7-3H-aldosterone in plasma, metabolic clearance rate and mean plasma concentration of aldosterone were measured by injecting 1, 2, 6, 7-3H-aldosterone before and after the treatment in 4 of the 7 patients with hyperthyroidism.
In hyperthyroidism, T3, T4, PRA and ASR were significantly higher (p<0.01), PAC relatively higher (p<0.05) and CPV remarkably lower (p<0.05) than in normal subjects. Increment of PRA and PAC after furosemide administration in an upright position was significantly greater in hyperthyroidism than in normal subjects but was normalized after the treatment. ASR showed higher levels compared to plasma concentrations in patients with hyperthyroidism than in normal subjects. The biological half-life of 1, 2, 6, 7-3H-aldosterone injected was 13.7 ± 1.2 minutes in the first phase and 31.0 ± 3.3 minutes in the second phase, apparently shorter than that in normal subjects. In particular, the half life in the second phase was significantly shortened in hyperthyroidism. The metabolic clearance rate was 1032.0±190.6 L/day/m2 in hyperthyroidism, significantly greater than that in normal subjects (826.4±96.4 L/day/m2). There were distinct negative correlations between CPV and T3 (r=-0.748, p<0.05), and CPV and PRA (r=-0.628, p<0.05), respectively, and a relative negative correlation between CPV and T4 (r=-0.567, 0.05<p<0.1), and CPV and PAC (r=- 0.508, 0.05<p<0.1) before and after the treatment in patients with hyperthyroidism.
From these results, it is suggested that the increased ASR in hyperthyroidism is caused by a hyperfunction of the renin-angiotensin system. However, the significant increase in metabolic clearance rate keeps PAC at high normal levels in spite of an increased ASR. After the treatment, PAC, ASR and metabolic clearance rate became normal. On the other hand, the distinct negative correlations between CPV and T3, CPV and T4, and CPV and PRA suggest that the decreased whole body fluid accompanied by hyperhidrosis is caused by a hyperfunction of the sympathetic nervous system.抄録全体を表示PDF形式でダウンロード (865K) -
第1報 正常妊婦尿中のPregnancy Specificβ1-Glycoprotein (SP1) の動態伊東 雅純1981 年 57 巻 10 号 p. 1508-1520
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーFor the purpose of studying the metabolic course of pregnancy, specific β1 -glycoprotein (SP1), the urinary protein in normal pregnancy was investigated and compared with SP1 in the serum.
1) Urine was concentrated 20-fold by ammonium sulphate precipitation, and SP1 was detected using immunological methods with commercially available anti-SP1 serum. By the gel immunodiffusion methods, one precipitation line was formed with the serum of pregnant women, but two lines were formed with their urine. By the single radial immunodiffusion method (SRID), a single-ring was detected in the serum, but a double-ring was detected in the urine.
When gel filtration of the concentrated urine was performed with a Sephadex G-150 gel column, there were two peaks for SP1 concentration. Each fraction of the two peaks showed a single-ring by SRID, but a double-ring was found at the intermediate fractions of the two. Furthermore, the mixture of each fraction of the two peaks formed a similar double-ring. SP1 in the fraction of the first peak (tentatively SP1 -A) was found to be the same as SP1 in the serum, and SP1 in the second peak (tentatively SP1-B) was specific to urine from pregnant women by the agar gel immunodiffusion method. This observation indicates that there are two kinds of SP1 in the urine, each having a different molecular weight. In the urine, SP1 -A forms an inner clear ring and SP1 -B forms an outer indistinct ring by SRID.
2) The concentrations of SP1 -A and SP1 -B in 10-fold concentrated urine were measured by SRID in 255 normal pregnant women at various gestational ages. These proteins gradually increased with gestation and reached the highest concentrations at the 10th month. The precipitation ring was found in 203 urine samples out of 255 cases (80%), and the double-ring was detected in 135 out of 255 cases (53%). The positive ratio of the double-ring increased until the 9th month of gestation but slightly decreased in the 10th month. The reason for this remains to be solved.
3) The expected daily excretion of SP1 was calculated from creatinin-corrected SP1 concentration. The excretion of SP1 -A increased gradually during the whole gestational period, while the excretion of SP1 -B increased slowly until the 5th month and then rapidly during the remaining gestational months. Both SP1 -A and SP1 -B were excreted the most in the 10th month.
4) The correlation coefficient was calculated between SP1 concentrations in the serum and urine. It was found that SP1 -B (r =0.63, p<0.001) correlated with SP1 in the serum more significantly than SP1 -A (r= 0.38, p<0.05).抄録全体を表示PDF形式でダウンロード (1615K) -
下田 新一, 鈴木 仁, 片山 宗一1981 年 57 巻 10 号 p. 1521-1530
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーIn order to clarify the possible participation of glucagon in glucose intolerance in myotonic dystrophy, six patients with myotonic dystrophy were examined. Three out of the six patients had an abnormal oral glucose tolerance curve. Two had frank diabetic glucose tolerance curves and the other had a high glucose value at 30 min after glucose loading, which fell beyond the normal range (Mean ± 2 S.D.). Total insulin response to oral glucose, calculated as insulin area, was significantly exaggerated (p<0.01 vs control value). In addition to these results, hyperglucagonemia was observed throughout the test in two patients with myotonic dystrophy. In the arginine infusion test, insulin response was not so exaggerated but plasma glucagon was significantly higher (p<0.05 vs control value) at each 30, 45, 60 minutes after arginine infusion, and the mean glucagon area under the curves was significantly greater (p<0.05 vs control value). None of the myotonic patients showed growth hormone or cortisol excess during an arginine infusion and/or insulin tolerance test. Three patients with myotonic dystrophy who had abnormal glucose tolerance curves showed absolutely or relatively high glucagon levels and exaggerated response to arginine infusion. These findings suggested that hyperglucagonemia might contribute to the appearance of glucose intolerance in myotonic dystrophy.抄録全体を表示PDF形式でダウンロード (840K) -
第1報 : 家兎および人睾丸・副睾丸のAndrogen Binding Protein大田 修平1981 年 57 巻 10 号 p. 1531-1539
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーAndrogen-binding protein (ABP) appears to originate in the Sertoli cells, which are stimulated by FSH and androgens, and serves to transport androgen into the lumen of the seminiferous tubules. Androgen may be more readily available for the process of spermatogenesis and sperm maturation.
ABP was measured in rabbit and human testes using the method of polyacrylamide gel electrophoresis described by Ritzen et al. (1974)
The values of ABP were as follows : rabbit testes (n=6) 0.44 ± 0.05 pmoles/mg protein, rabbit caput epididymides (n=5) 5.52 ± 1.4 pmoles/mg protein, rabbit corpus epididymides (n=5) 1.35 ± 0.43 pmoles/mg protein, rabbit cauda epididymides (n=5) 0.39 ± 0.12 pmoles/ mg protein, rabbit efferent duct fluid (n=2) 62.4 ± 24.6 pmoles/mg protein and human testes (obtained from untreated patients with prostatic cancer) (n=3) 0.30 ± 0.22 pmoles/mg protein.
Since we could not distinguish ABP from testosterone-estrogen binding globulin (TeBG) with the assay, the values were corrected at first by Hb concentration, but it was not satisfactory, and other methods are now being studied抄録全体を表示PDF形式でダウンロード (620K) -
羽場 利博1981 年 57 巻 10 号 p. 1540-1553
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーThe catabolic rate of low density lipoprotein (LDL) has been shown to be delayed in hypothyroidism and increased in hyperthyroidism. LDL catabolism is mainly mediated through the LDL receptor pathway in the parenchymal cells. In the present paper the effect of thyroid hormones on LDL receptors was studied in cultured human skin fibroblasts.
Human fibroblasts were established form 2 normal subjects, one heterozygous patient and 2 homozygous patients with familial hypercholesterolemia (FH). Human LDL (density 1.020 -1.050 g/ml) from the serum of healthy subjects was prepared by differential ultracentrifugation. 125I-LDL was prepared by the IC1 method of MacFarlane. Prior to incubation with 125I-LDL, cells were preincubated in a medium containing 5% lipoprotein-deficient serum obtained from a myxoedematous patient.
Uptake and degradation of LDL by normal cells and FH heterozygous cells increased about 20% with 1.0 μg/ml of L-triiodo-thyronine (T3) or L-thyroxine (T4). In FH homozygous cells, no increase of LDL receptor activity was observed with T3 or T4.
These results suggest that thyroid hormones regulate LDL catabolism through the LDL pathway.抄録全体を表示PDF形式でダウンロード (1194K) -
松崎 茂1981 年 57 巻 10 号 p. 1554-1566
発行日: 1981/10/20
公開日: 2012/09/24
ジャーナル フリーChanges in polyamine contents and in the activity of polyamine biosynthetic enzymes were studied in the rat thyroid. Both exogenous and endogenous TSH promptly stimulated the activity of thyroid ornithine decarboxylase (ODC) and S-adenosyl-L-methionine decarboxylase. Treatment of the animals with antithyroid drugs resulted in an increase in circulating TSH level, the activities of the enzymes and thyroidal contents of putrescine, spermidine and spermine. Theophylline, which stimulated methylthiouracil (MTU) -induced goitrogenesis without increasing circulating TSH level, increased the thyroid ODC activity. In contrast, treatment with excess iodine (KI) which induced thyroid involution without affecting circulating levels of TSH, T4 and T3 resulted in a rapid reduction in the thyroid ODC activity. The MTU-induced goiter involuted after MTU withdrawal and the thyroid ODC and polyamine contents decreased within a few days after its withdrawal.
The activity of thyroid arginase was increased by MTU and decreased by T4 injection. The enzyme may have some regulatory role in polyamine biosynthesis by controlling ornithine supply.
Polyamine levels were elevated in both papillary and follicular adenocarcinomas of the human thyroid. The results suggest that the measurement of thyroid polyamines may be useful for the diagnosis of carcinomas.抄録全体を表示PDF形式でダウンロード (1013K)
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