日本内分泌学会雑誌
Online ISSN : 2186-506X
Print ISSN : 0029-0661
ISSN-L : 0029-0661
58 巻, 1 号
選択された号の論文の6件中1~6を表示しています
  • 野間 剛, 下澤 和彦, 櫻田 則之, 矢田 純一, 紫芝 良昌, 小沢 安則, 稲月 文明
    1982 年 58 巻 1 号 p. 1-8
    発行日: 1982/01/20
    公開日: 2012/09/24
    ジャーナル フリー
    Anti-thyroglobulin antibody forming cells (AFC) were generated from the cultured peripheral lymphocytes of patients with chronic thyroiditis. In order to clarify whether this reflects the antibody formation in vivo, the number of antibody forming cells generated in the culture was related to the serum antibody titer in 121 cases.
    There was no case which produced more than 10 AFC from 2×105B-cells in the group with the low serum antibody titer (less than 102), while 29.4% produced more than 10 AFC in the patients with antibody titer of 202 and 204 and 65.2% in the group with titer of 802-6402. The average number of AFC of the last group was 31.4 ± 17.7 (SD).
    AFC and serum antibody titers were estimated on various occasions with the same patients. A good relationship was observed between these two parameters.
    There was no significant correlation between the numbers of AFC and serum antimicrosome antibody titers.
  • 中村 重徳, 伏見 勝正, 奥山 牧夫, 三浦 清
    1982 年 58 巻 1 号 p. 9-23
    発行日: 1982/01/20
    公開日: 2012/09/24
    ジャーナル フリー
    Anti-thyroid hormone autoantibodies were found in two sisters of short stature (case 1 : 13 years old, case 2 : 10 years old). Physical examination revealed clinical findings of hypothyroidism with diffuse goiter. A diagnosis of Hashimoto's thyroiditis was made by open biopsy of the thyroid gland in both patients. Serum levels of T3 and T4 were assayed by double antibody radioimmunoassay. In case 1, serum T3 level was 16 ng/dl and serum T4 level was 2.0 μg/dl. In case 2, serum T3 levels were high (range, 375-4660 ng/dl), while serum T4 remained at a very low level (0.9μg/dl). On the other hand, the level of T3 in case 2 as well as the level of T4 in case 1 was not detectable by a single antibody radio-immunoassay using dextran-coated charcoal or polyethylene glycol separation. In each case, the serum TSH level measured by double antibody radioimmunoassay was extremely high (235 μU/ml in case 1,240 μU/ml in case 2), which was compatible with the clinical features of primary hypothyroidism. Sera from both patients were incubated with 125I-T3 and 125I-T4, followed by precipitation with polyethylene glycol. The binding of 125I-T3 with serum from case 2 and the binding of 125I-T4 with serum from case 1 were markedly high, suggesting the presence of T3-and T4-binding substance (s) in the sera. IgG prepared from the sera of both cases showed marked and specific binding with T3 in case 2 and T4 in case 1. The association constant for T4-antibody in case 1 was 5.2×108M-1 and for 3-antibody in case 2 was 5.0×109M-1. The binding capacity for T4 was 1.2 ng/mg.IgG and for T3 was 0.3 ng/mg.IgG.
  • Renin-Angiotensin系と電解質代謝との関係
    砂田 和彦
    1982 年 58 巻 1 号 p. 24-32
    発行日: 1982/01/20
    公開日: 2012/09/24
    ジャーナル フリー
    It is well known that the alternations on humoral homeostasis such as a decrease of pH, an elevation of PaCO2 and changes of electrolytes have been detected during respiratory acidosis. The author is of the opinion that the renin-angiotensin-aldosterone system might be related to these changes during respiratory acidosis.
    Recently, Fujii and Morita have reported that an increase in plasma renin activity appeared during acute respiratory acidosis. These reports prompted me to question whether the renin-angiotensin-aldosterone system was related to the pathophysiological evidence concerning the respiratory acidosis.
    it is generally accepted that plasma aldosterone concentration is controlled by (1) the renin-angiotensin system, (2) ACTH and (3) serum potassium. Therefore, the purpose of this experiment was to investigate the roles of the renin-angiotensin system and electrolyte metabolism on plasma aldosterone concentration during acute respiratory acidosis.
    I initiated acute respiratory acidosis with 10% CO2 inhalation in healthy mongrel dogs, and then plasma aldosterone concentration, plasma renin activity, electrolytes and cardio-renal hemodynamics were measured.
    The results were as follows :
    1. The increase of plasma aldosterone concentration was delayed and blurred, in contrast with a significant increase of plasma renin activity, during the acute respiratory acidosis, due to the delayed time course of angiotensin II stimulation and also the expected changes in the concentration of angiotensin II receptors on the zona glomerulosa in the adrenal cortex.
    2. There was a possibility that the increase in the reabsorption of sodium and water in the proximal tubules, and the increase of ADH secretion during the acute respiratory acidosis, could be related to a decrease in excreted sodium and potassium as well as urine volume.
    These results suggest that the changes of plasma aldosterone concentration may be affected partially by renin-angiotensin system but not by serum potassium, and plasma aldosterone should be a minimal determinant on the electrolyte metabolism during respiratory acidosis with 10% CO2 inhalation.
  • 工藤 秀機
    1982 年 58 巻 1 号 p. 33-48
    発行日: 1982/01/20
    公開日: 2012/09/24
    ジャーナル フリー
    The effect of testosterone propionate (TP) or estradiol-17β (E2) on the activities of thymidine kinase (TK) and tissue plasminogen activator (Act) in the prostates of 22 day old immature SD rats was studied.
    The Tailor and fibrin plate methods were applied to measure the activities of TK and Act respectively. Act activity was expressed in terms of the Urokinase International Unit.
    After a single TP injection im, TK activity began to increase at 24h and peaked at 48h followed by a rapid decrease. The maximum activity, 40.0 ± 11.5 pmol/mg protein/15 min, was 50 times higher at 48 h and peaked at 96 h, with a subsequent decline to the control value within 14 days. The maximum Act activity, 5.1 ± 0.4 U/prostate, was 1.6 times the control value.
    The activities of both TK and Act altered depending on TP doses. 1 mg/100 g BW of TP brought about their maximum activities at 48 h and 96 h respectively.
    Three TK isozymes were obtained from the prostates by DEAE-cellulose column chromatography. Fraction A, which was eluted in OM NaCl, differed from other isozymes in that its activity was only slightly suppressed by dCTP and was elevated most conspicuously after TP injection.
    Elevated activities of both TK and Act after TP injection were completely mhibitecl by Puromvcin or Actinomycin D.
    The results of the present experiment demonstrated that TK and Act were synthesized in the prostatic cells after TP injection and were androgen dependent.
    E2 did not inhibit the elevation of TK and Act activities by TP injection. In the present study estrogen showed a synergetic effect with androgen on these two activities, especially TK, in the rat prostate.
  • 田村 貴, 水上 尚典, 玉田 太朗
    1982 年 58 巻 1 号 p. 49-62
    発行日: 1982/01/20
    公開日: 2012/09/24
    ジャーナル フリー
    Several investigators have reported that CB-154 induces ovulation in patients with normoprolactinemic anovulation as well as those with hyperprolactinemic amenorrhea. In the present research, the ovulation-inducing effects of CB-154 were studied in normoprolactinemic subjects with special reference to the feedback effect of estradiol on LH release. Thirty female subjects aged 20-32 years with ovulatory disturbances were studied. Basal serum PRL, LH and FSH were determined by radioimmunoassay, and both hyperprolactinemic and hypergonadotropic anovulatory patients were excluded.
    A 2mg dose of estradiol benzoate was administered intramuscularly to each subject and 8ml samples of venous blood were taken at 0, 6, 24, 30, 48, 54, 72, 78 hr. The subjects under study were divided into two groups, A and B, according to the effect the estradiol benzoate had on LH release. Group A subjects (nine in all) failed to show any positive feed-back release of LH in response to the estradiol benzoate. Group B subjects (twenty-one in all) showed a more than twofold increase in circulating LH as compared with the initial serum LH value, and this was taken as an indication of positive feedback release. All the subjects in group B were given clomiphene (50-100mg daily for five days). The clomiphene therapy was effective in eleven subjects, and four became pregnant (three in the first or second cycle of treatment and one in the third). The therapy was ineffective in the remain-ing six subjects, four of whom were diagnosed as suffering from polycystic overy syndrome. Clomiphene was judged to be effective when the subjects undergoing therapy with this drug ovulated during three successive treatment cycles, and ineffective when the subjects did not fulfill this criterion (criterion for effectiveness of clomiphene). With the exception of four cases of polycystic ovary syndrome and three pregnancies which occurred in the first or second cycle, the rate of effectiveness of clomiphene in group B was 12 our of 14. It was concluded from these results that clomiphene was effective in group B subjects except in cases of polycycstic ovary syndrome. Treatment with clomiphene alone was effective in none of the seven subjects in group A. However, administration of CB-154 for several weeks prior to and during the clomiphene treatment cycle (combined therapy of CB-154 and clomiphene) led to remarkably improved ovulation rates in five subjects in gorup A. Four patients in group A were selected, and given estradiol benzoate prior to (control) and during (study) CB-154 administration. In each case administration of CB-154 elicited marked posi-tive feedback release of LH as compared with the control period, that is to say, CB-154 transformed group A patients into group B patients. This effect of CB-154 may explain why therapy combining CB-154 and clomiphene improved ovulation rates in group A. We concluded from these results that CB-154 restored the positive feedback release of LH in the group A subjects so that they ovulate on therapy which combines CB-154 and clomiphene.
  • 多賀 理吉, 武谷 雄二, 森 宏之, 水口 弘司, 木川 源則, 坂元 正一
    1982 年 58 巻 1 号 p. 63-72
    発行日: 1982/01/20
    公開日: 2012/09/24
    ジャーナル フリー
    Primary amenorrhea, in which serum concentrations of gonadotropins are low or normal, has been considered to be relatively rare. Recent analysis in our outpatient clinic revealed that the incidence of hypothalamic primary amenorrhea is higher than previously appreciated, comprising 26.7% of the total primary amenorrheas. Endocrinological functions of the hypothalamo-hypophyseo-ovarian axis in these patients were therefore investigated. Studies on gonadotropin secretion indicated that a disturbance in LH-RH secretion and a lack of estrogen positive feedback were principle features of this disease. In addition to the dysfunction of gonadotropin secretion, the control of PRL secretion was disturbed in this disease since there was a poor PRL response to chlorpromazine in spite of normal responsiveness to TRH. Thus, “isolated gonadotropin deficiency” hitherto used to describe this disease is not pertinent. Furthermore, these results suggest that PRL might be involved in the onset of puberty in humans. Clinically, the induction of ovulation with HMG (Human Menopausal Gonadotropin) is possible in hypothalamic primary amenorrhea, although the ovarian responsiveness to gonadotropin is poor. Therefore, an endocrinologically precise diagnosis of hypothalamic primary amenorrhea is important in the gynecological clinic because fertility has been considered to be almost impossible in primary amenorrhea.
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