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65 巻, 8 号
選択された号の論文の11件中1～11を表示しています
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中村 隆彦, 大槻 眞, 岡林 克典, 藤井 正俊, 谷 聡, 岡 徹, 藤澤 貴史, 馬場 茂明1989 年 65 巻 8 号 p. 695-703
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーNeuromedin B and neuromedin C are novel decapeptides that have recently been isolated from porcine spinal cord and canine intestinal mucosa. We have studied the effects of neuromedin B and neuromedin C on insulin release from the isolated perfused rat pancreas. The effect of neuromedin B was detectable at a concentration of 10mM, and that of neuromedin C was detectable at a concentration of 1 nM. Further increase in the concentration of neuromedin B and neuromedin C resulted in dose-dependent increases in insulin secretion. The effectiveness of neuromedin B and neuromedin C as insulinotropic agents depended on the glucose concentration; both were more effective at a higher concentration of glucose. However, insulinotropic effects of these peptides in the presence of 8.3mM glucose were limited to the first 3 min of a 20-min perfusion. These results, coupled with a recent study demonstrating bombesin-like immunoreactivity in nerves in the pancreas, suggest that neuromedin B and neuromedin C exert a direct local action on insulin secretion in the pancreas.抄録全体を表示PDF形式でダウンロード (848K) -
鈴木 明, 橋野 正史, 千葉 博, 斉藤 裕, 野嶽 幸正, 矢内原 巧, 中山 徹也1989 年 65 巻 8 号 p. 704-714
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーIt is known that both catecholamines (CA) and cortisol (F) levels elevate during labor. To determine the correlation between adrenal steroids and medullary function, maternal blood was collected during pregnancy, first stage of labor (MVI) and at delivery (MVII). Umbilical arterial and venous blood (UA, UV) was also obtained at delivery. Further, ACTH or dexamethasone (Dx) was given during the first stage of labor, and maternal blood was collected before and 30 minutes after the administration. Plasma levels of CA [noradrenaline (NA), adrenaline (Ad)] were extracted by trihydroxyindole method and were measured by HPLC. DHA-S and F levels were determined by specific RIA.
Results are as follows :
1. No apparent change was observed in maternal NA and Ad levels throughout pregnancy. DHA-S levels were high in first trimester and decreased as pregnancy advanced, while F levels showed an increase trend as pregnancy progressed.
2. All hormone levels in maternal blood increased remarkably during labor. A significant negative correlation between F and Ad levels at delivery was noted. When F levels were elevated by ACTH administration, Ad levels decreased. Ad levels elevated when F levels were suppressed by Dx administration.
3. NA, Ad and DHA-S levels in cord blood were higher than those in MVII. Levels of F in maternal blood were higher than those in cord blood. A significant correlation of F in MVII and UA was observed. These results indicate that the suppressive effect of F may be involved in the mechanism of Ad secretion, though the secretion of Ad increased with F in the course of labor. The response of fetal adrenal to the stress of labor may be different from that of maternal adrenal since a significant correlation was not noticed between the levels of Ad and cortisol in cord blood as was found in maternal blood.抄録全体を表示PDF形式でダウンロード (957K) -
高倉 健, 犀川 哲典, 米持 英俊, 伊東 祐信, 高木 良三郎1989 年 65 巻 8 号 p. 715-727
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーPDF形式でダウンロード (1331K) -
船越 徹, 森川 肇, 上田 康夫, 望月 眞人1989 年 65 巻 8 号 p. 728-742
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーIn order to clarify the potential role of IGF-I in fetal growth, the dynamics of IGF receptor were investigated in comparison with insulin receptor in human and rat fetuses.
In humans, the serum levels of IGF-I measured by IGF-I radioimmunoassay after acid-ethanol extraction were significantly lower in fetuses than in adult controls. Conversely, serum insulin levels in fetuses were not indistinguishable from those in adults. On the other hand, specific binding of 125 I-IGF-I to human fetal erythrocytes was 2.3%, which was significantly higher than that of adult women (1.6%). Insulin binding to the erythrocytes was also higher in human fetuses than in adult controls (cord : 3.8%, adult : 3.0%). By Scatchard analysis, the changes in the specific binding of IGF-I and insulin were mainly due to the changes in the binding capacity rather than those in the binding affinity. Additionally, IGF receptor on human fetal erythrocytes was recognized as type I IGF receptor, because the binding of 125I-labelled IGF-I and 125 I-labelled IGF-II could be displaced by the addition of cold IGF-I, IGF-II and insulin.
In rats, serum levels of IGF-I were also much lower in fetuses than in adult controls. Specific binding of 125 I-IGF-I to liver microsomal membranes was 34.3% (per 400/tg protein) in fetal rats (D20), which was significantly higher than that of adult rats (3.2%). Scatchard analysis indicated that these changes in 125 I-IGF-I binding was chiefly due to the changes in binding capacity. On the other hand, serum insulin levels increased with progress of pregnancy, and specific binding of insulin to microsomal membranes of fetal liver increased on D21 of gestation, mainly due to the increase in binding affinity rather than binding capacity.The bindings of 125 I-labelled IGF-I and 125 I-labelled IGF-II to fetal liver microsomal membranes were clearly displaced by cold IGF-I and IGF-II but not by excess of cold insulin, indicating that the receptor was type 2 IGF receptor.
These results suggest that IGF-I possesses much more receptor in fetal tissues than in adult tissues despite its lower concentration in fetal sera. It is speculated that IGF-I may play an important role in fetal growth and development.抄録全体を表示PDF形式でダウンロード (1376K) -
コレラ毒素及び, 百日咳毒素の効果的崎 尚, 坂本 長逸, 長尾 宗彦, 西崎 朗, 誉田 芳孝, 馬場 茂明1989 年 65 巻 8 号 p. 743-749
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーWe have recently shown that F- can mimic the actions of cholecystokinin (CCK) on amylase release, Ca2+ mobilization and inositol phosphate generation in pancreatic acinar cells. We have concluded, therefore, that pancreatic CCK receptors may be coupled to the activation of polyphosphoinositide hydrolysis by a guanine nucleotide regulatory protein (N protein), which seems to be sensitive to F-. In the present study, in order to further characterize this N protein coupled to pancreatic CCK receptors, we have examined the effects of bacterial toxins, pertussis toxin (PT) and cholera toxin (CT) on both CCK-and NaF-induced cellular responses in isolated rat pancreatic acini. Neither PT or CT pretreatment of acini affected both CCK-and NaF-stimulated increases in intracellular Ca2+ concentration monitored by quin2. Furthermore, pretreatments of acini with PT and CT didn't alter the effects of CCK on inositol phosphate generation in acini. Similarly, NaF-induced inositol phosphate generation was not changed by these toxin treatments. However, pretreatment procedures employed in this study were considered to catalize complete ADP-ribosylation of α-subunit of the stimulatory (Ns) and inhibitory (Ni) N protein. These results, therefore, strongly suggest that a N protein coupling pancreatic CCK receptors to the breakdown of polyphosphoinositide may be distinct from Ns or Ni like protein.抄録全体を表示PDF形式でダウンロード (1095K) -
大東 茂樹, 小崎 俊男, 橋野 正史, 矢内原 巧, 中山 徹也, 大西 修平, 大沢 由男1989 年 65 巻 8 号 p. 750-761
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーTo study the serum levels of 19-hydroxyandrostenedion (19-0H-A), known as an obligatory intermediate of estrogen biosynthesis and considered to be one of the hypertensinogens, a method using GC-MS with application of synthesized [7, 7-d2] androstenedione (A), [7, 7-d2] 19-OH-A and [9, 11-d2] estrone (E1) as internal standards was newly developed. Normal pregnant women and pregnant women complicated with hypertension near term were selected for the study. The levels of 19-OH-A and E1 increased significantly as gestation progressed [19-OH-A; 224.7 ± 72.1 (1st trimester), 655.5 ± 325.4 (2nd trimester). 1517.8 ± 543.6 (3rd trimester) pg/ml], and a positive correlation was found between the levels of the two steroids. No apparent change was observed in A levels during the course of pregnancy. The mean levels of 19-OH-A in pregnancy complicated with hypertension at 2nd and 3rd trimester were 761.7 ± 348.9 and 1473.0 ± 491.4pg/ml, which were compatible with those in normal pregnancy. The levels of 19-OH-A at delivery in maternal vein (MV) were 1735.1 ± 683.9pg/ml. Significantly higher levels of 19-OH-A were found in umbilical vein (UV) (1977.2 ± 564.9pg/ml) than those in umbilical artery (109.7 ± 49.1pg/ml). 19-OH-A concentration in term placental tissue was 16.3 ng/g.w.w. tissue.
This is the first report to demonstrate the serum 19-OH-A levels measured by GC-MS and also to demonstrate the levels in the cord blood. The results indicate that 19-OH-A may be the product of pregnancy and may be derived from the feto-placental compartment.抄録全体を表示PDF形式でダウンロード (1068K) -
灌流研究高尾 文子1989 年 65 巻 8 号 p. 762-770
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーTo evaluate the effect of maternal hyperglycemia on insulin secretion by neonatal rat B cells, perifusion was conducted on monolayer cultures of rat neonates from normoglycemic (C) mothers or those made slightly (SH) and highly (HH) hyperglycemic by the injection of streptozotocin. In the C, SH and HH groups, B cells responded to 16.7 mM glucose, 10mM leucine, 10 mM 2-ketoisocaproate and 10 mM arginine in a biphasic manner, although these nutrients provided stimuli so feeble that insulin secretion in the second phase was slightly raised above basal levels. However, quantitative relationships differed. Compared with the C group, B cells in the SH group showed a significant increase in the secretion of the second phase in response to glucose, 2-ketoisocaproate and arginine but not to leucine, whereas there was no difference in the secretion of the first phase between these two groups. In the HH group, a lower insulin secretion of the first and second phase was observed. These results are compatible with hyperglycemia-hyperinsulinaemia theory concerning the function of B cells in neonates born to slightly hyperglycemic mothers and with a defect in insulin secretion by neonates from highly hyperglycemic females.抄録全体を表示PDF形式でダウンロード (718K) -
村上 司, 野口 志郎, 村上 信夫, 加藤 亮二, 太田 康幸1989 年 65 巻 8 号 p. 771-780
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーThe levels of serum calcium (Ca), inorganic phosphate (P) and mid-molecular parathyroid hormone (PTH) were measured in 37 patients with Graves' disease (12 in hyperthyroid state, 25 in euthyroid state followed by subtotal thyroidectomy), 6 with papillary carcinoma of the thyroid, 8 with benign nodular goiter and 19 healthy control subjects in order to investigate the change in these levels before and after thyroidectomy.
The levels of serum Ca and P of the hyperthyroid patients with Graves' disease were 9.73 ± 0.30 mg/dl and 4.47 ± 0.44 mg/dl, respectively, which were significantly higher than those of healthy control subjects. No significant difference in the levels of serum PTH was observed between hyperthyroid patients with Graves' disease and healthy control subjects. The levels of serum Ca, P and PTH of euthyroid patients with Graves' disease were not significantly different from those of healthy control subjects.
In the patients with Graves' disease who had undergone subtotal thyroidectomy followed by postoperative tetany, serum Ca and serum PTH decreased significantly from 9.39 ± 0.45 mg/dl to 7.90 ± 0.33 mg/dl and from 406.6 ± 164.4 pg/ml to 229.9 ± 136.0 pg/ ml, respectively, after surgery, but there was no change in serum P. In the patients without postoperative tetany, serum Ca and serum P decreased significantly after surgery from 9.65 ± 0.36 mg/dl to 9.15 ± 0.33 mg/dl and from 4.03 ± 0.46 mg/dl to 3.47 ± 0.54 mg/dl, respectively, without any change in the levels of serum PTH.
In the patients with papillary carcinoma or benign nodular goiter without postoperative tetany, the levels of serum Ca, P and PTH did not change after surgery. In the patients with papillary carcinoma followed by postoperative tetany, serum Ca decreased significantly after surgery with concomitant decrease of serum PTH.
It was concluded that excessive thyroid hormones influenced Ca metabolism, and the transient tetany following subtotal thyroidectomy for Graves' disease seemed to be due to both the absorption of Ca by hungry bone and parathyroid hypofunction.抄録全体を表示PDF形式でダウンロード (822K) -
治療前後におけるアポ蛋白の検討織部 尚利1989 年 65 巻 8 号 p. 781-793
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーThe present study was undertaken to assess lipid metabolism in patients with thyroid dysfunction with special reference to serum apolipoprotein levels. Serum lipid, lipoprotein and apolipoprotein levels were determined in 28 hyperthyroid and 16 hypothyroid female patients while untreated and euthyroid. Apolipoproteins were measured by the method of single radial immuno-diffusion (SRID). These results were compared with the values of 28 female controls. In the untreated hyperthyroid group, the serum levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were significantly decreased compared to the controls and increased after treatment. In hypothyroidism, these values before treatment were higher than those in the controls and decreased after treatment. Serum apo A-I, A-II, B and C-III levels were significantly decreased in the untreated hyperthyroid group compared to the control values. Apo C-II and E levels in hyperthyroidism were identical both before and after treatment compared with the control values, respectively. In the untreated hypothyroidism, apo B, C-II, C-III and E levels were significantly elevated compared to the controls, and these changes in apolipoproteins except apo C-II were restored after treatment. Apo A-I and A-II levels in the untreated hypothyroidism were not statistically different from the values after treatment or those in the control group. Serum thyroid hormone (T3, T4) levels inversely correlated apo B and C-III in all subjects.
In hypothyroidism, serum TSH positively correlated with apo B, C-II and C-III. The increase in relative body weight (%RBW) in hyperthyroidism during treatment correlated with the changes of TC and LDL-C.
In conclusion, these results indicate that thyroid hormones have a substantial influence on the serum apolipoprotein levels, and that measurement of apolipoproteins as well as lipids and lipoproteins in patients with thyroid dysfunction may be useful to evaluate the lipid metabolism and the effect of therapy.抄録全体を表示PDF形式でダウンロード (1220K) -
佐藤 清貴, 長谷川 延広, 木川田 隆一, 増田 卓, 丸茂 文昭1989 年 65 巻 8 号 p. 794-806
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーWe have established an easily reversible acute heart failure model in beagle canines by reversible aortic or mitral regurgitation (AR or MR). To cause reversible AR, a basket catheter was inserted into the left ventricle from the apex and fixed at the aortic valve in 10 canines. To cause MR, a basket catheter was inserted into the left atrium via the pulmonary vein and fixed at the mitral valve in 10 canines. The regurgitation by AR or MR was caused by extending the tip basket wire, and the recovery from the regurgitation was immediately possible by closing it. Left atrial pressure (LAP), right atrial pressure (RAP) and pulmonary artery pressure (PAP) were increased significantly during AR or MR, and decreased to the normal level after the release of AR or MR.
Using these reversible acute heart failure models, the effects of both advancing and restoring acute heart failure by the secretion of ANP were examined by observing the changes of ANP concentration and its molecular forms in the plasma and left atrial tissue in the same canine. Plasma ANP concentration showed a reversible change. In group analysis, plasma ANP concentration did not correlate with LAP or RAP, but in each canine it showed high correlations with LAP (r=0.70-0.94, 0.82 ± 0.07) and RAP (r=0.60-0.93, 0.79± 0.08), having a different slope in each regression line. The ANP concentration in the atrial tissue was decreased during AR or MR, but the low level was maintained after AR or MR. The main molecular form of ANP in the plasma was alpha-ANP and that in the tissue was gamma-ANP.
In summary, the tissue storage of ANP was decreased because the ANP secretion caused by stimulation of acute heart failure exceeded its production. The ANP secretion was decreased by the subsequent elimination of heart failure, but the production was not stimulated rapidly, because the tissue content remained unchanged.抄録全体を表示PDF形式でダウンロード (1111K) -
深瀬 正晃, 藤田 拓男, 松本 俊夫, 尾形 悦郎, 飯島 隆, 竹沢 順一, 斎藤 健一, 石毛 英幸, 藤本 正雄1989 年 65 巻 8 号 p. 807-827
発行日: 1989/08/20
公開日: 2012/09/24
ジャーナル フリーParathyroid hormone radioimmunoassay (RIA), specific for mid-region of the PTH molecule, has been proven to be extremely useful for the differential diagnosis of abnormal calcium metabolism.
Recently, we developed a highly sensitive RIA for PTH, consisting of PTH antiserum (CH9), 125I labelled Tyr42 hPTH (43-68) and synthetic hPTH (1-84) as standard. This MA cross-reacted with mid-region and carboxyl terminals of PTH. The within-assay and betweenassay coefficients of variation were less than 4.6% and less than 8.6%, respectively. The limit of detection was 50pg/ml. The levels of serum calcium, serum phosphate, serum creatinine, Tmpo4/GFR and creatinine clearance (Ccr) in normal healthy volunteers aged 20 to 50 years remained almost constant and showed 9.24 ± 0.34mg/dl (mean ± SD, n = 242), 3.34 ± 0.38mg/dl (n = 242), 0.870 ± 0.121mg/dl (n= 242), 3.20 ± 0.54mg/dl GF (n=189) and 103 ± 17ml/min (n = 137), respectively. All healthy volunteers (n = 326) had measurements of PTH in the blood. From 20 to 50 years, normal PTH mean was 374 ± 97pg/ml (± SD, n= 237) and ranged from 180-568pg/ml, and from 60 to 80 years it was 471± 133pg/ml (n = 34) and ranged from 205-737pg/ml.
Since we found that PTH was markedly elevated above normal when Ccr was below 40ml/min, and PTH was very significantly correlated with the reciprocal of Ccr (r = 0.8996, P<0.001) using a multivariate analysis, all of the patients whose Ccr was higher than 40ml/ min were selected and examined in the following studies. Serum PTH values completely separated patients with surgically proven primary hyperparathyroidism (1°HPT) from malignant associated hypercalcemia (MAH), and patients wih idiopathic hypoparathyroidism (IHP) from pseudohypoparathyroidism (PHP), both of which were diagnosed by Ellsworth-Howard test. PTH values in all of the patients with 1°HPT (n = 23) were above normal, but those with MAH (n=6) were below the normal or lower normal range. PTH values in patients with PHP (n = 7) showed above normal, while those with IHP (n = 5) were below the normal range. PTH was normalized in post operative status in all patients after parathyroidectomy (n = 6).
These results indicate that this PTH RIA is extremely useful for the differential diagnosis in diseases with calcium abnormalities.抄録全体を表示PDF形式でダウンロード (1489K)
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