巻号一覧
前身誌
67 巻, 12 号
選択された号の論文の5件中1～5を表示しています
- |<
- <
- 1
- >
- >|
-
山田 隆司1991 年 67 巻 12 号 p. 1295-1308
発行日: 1991/12/20
公開日: 2012/09/24
ジャーナル フリーAs an immunologic process, TSH receptor antibody (TRab) may be synthesized by the lymphocytes within the thyroid gland. Two techniques were devised to express this TRap activity: a) thyroid stimulating immunoglobulin (TSI) expressed as thyroid c AMP synthesis and b) TSH binding inhibiting immunoglobulin (TBII) expressed as TSH displacement. In general, serum TSI and TBII activity correlated well with each other in hyperthyroid patients. Measurement of TSI and TBII is useful to assess improvement of immunologic abnormality induced by antithyroid drugs. The data so far gathered indicated that TRab disappears from the blood in about 70-80% of hyperthyroid patients in response to antithyroid drugs. However, this does not indicate that intrathyroidal TRab synthesis has ceased, since thyroglobulin is still secreted supernormally and T3 fails to suppress the thyroid in some patients. On the other hand, normalization of thyroglobulin and absence of TRab in the blood indicated complete normalization in the patients as evidenced by positive T3 suppressibility. By using a number of new drugs, further efforts must be made to completely normalize immunologic abnormality in 100% of patients. Recurrence of hyperthyroidism of Graves' disease is generally associated with re-appearance of TRab in the blood in most of the recurrent patients after discontinuation of antithyroid drugs.抄録全体を表示PDF形式でダウンロード (1296K) -
In situハイブリダイゼーション法をはじめとする分子細胞化学の進歩と内分泌学研究への応用河田 光博1991 年 67 巻 12 号 p. 1309-1318
発行日: 1991/12/20
公開日: 2012/09/24
ジャーナル フリーIn situ hybridization is a histochemical technique that attracts many cell biologists and others interested in developmental biology, virology, genetics and neuroendocrinology. This method gives us the precise localization and identification of individual cells which contain specific nucleic acid sequences, in a similar manner to the immunohistochemistry of cells which have a particular protein. There has been a wide range of applications for this technique. One of the most important and significant applications of in situ hybridization is the demonstration of specific mRNA in particular cells. This is quite valuable in heterogeneous tissue such as that of the hypothalamus with a various types of different cells. The combination with the immunohistochemistry enables us to study the dynamics of peptides or proteins in a certain tissue or cell. In this mini-review the logic and methodology of molecular cytochemistry, particularly in situ hybridization, with its application in the endocrinological field was presented.抄録全体を表示PDF形式でダウンロード (2135K) -
高島 正樹, 森川 肇, 山崎 峰夫, 望月 眞人1991 年 67 巻 12 号 p. 1319-1338
発行日: 1991/12/20
公開日: 2012/09/24
ジャーナル フリーIn order to clarify the pathophysiological significance of changes in intracellular ionized calcium and sodium levels in pregnancy induced hypertension (PIH), the intracellular ionized calcium concentration in platelets (p-[Ca2+]i) and the intracellular ionized sodium concentration in red blood cells (r-[Na+]i) were measured simultaneously in PIH women in the third trimester. p-[Ca2+]i in the first trimester showed a slightly greater increase than in the women of normal luteal phase. In the second trimester, p-[Ca2+]i decreased significantly compared to first trimester, and the third trimester and first trimester levels were the same. In women with mild and severe FIR, the levels in both groups were significantly increased compared with that in normal pregnant women. Thus mechanisms not associated with platelet activation were considered as the cause of the increase of p-[Ca2+]i of women with PIH. r-[Na+]i in mild and severe PIH were also significantly increased compared to normal pregnancy. No correlation between p-[Ca2+]i and r-[Na+]i and diastolic blood pressure was observed in normal pregnancy, but a positive correlation was observed in PIH. When the male platelets were incubated with serum from non-pregnant or normal pregnant women, p-[Ca2+]i did not show any significant changes. On the other hand, p-[Ca2+]i was significantly increased after the incubation with serum from PIH women. Moreover, p-[Ca2+]i was significantly increased after the incubation with 17β-estradiol, parathyroid hormone (PTH), or endothelin-1 (ET-1). These data suggest that the increase of p-[Ca2+]i and r-[Na+]i in PIH is important in the initiation and maintenance of hypertension by influencing peripheral vascular resistance, and also various factors in the serum of PIH women may contribute to the accumulation of intracellular ionized calcium in patients with PIH.抄録全体を表示PDF形式でダウンロード (1984K) -
森 一弥, 新村 進, 水野 兼志1991 年 67 巻 12 号 p. 1339-1344
発行日: 1991/12/20
公開日: 2012/09/24
ジャーナル フリーIt has been suggested that autoimmunity and genetic factors may play a specific role in the development of idiopathic hypoparathyroidism.
We reported a case of idiopathic hypoparathyroidism complicated with chronic thyroiditis. The patient, a woman 40 years old, visited our clinic because of tetany of both hands and dizziness. She was of short stature with a round face. She also had a goiter, hypocalcemia, hyperphosphatemia and decreased parathyroidal function, but renal function was normal. Her TSH level was slightly high with a positive microsome test (×1600), and the levels of thyroid hormones tended to be low. Based on Ellsworth-Howard test findings, a diagnosis of idiopathic hypoparathyroidism was made, with the complication of chronic thyroiditis confirmed by the thyroidal biopsy. Administration of 1α-OH-D3 normalized the level of serum calcium. No special treatment was given for the chronic thyroiditis in order to observe its natural course. Her TSH returned to normal, and the level of thyroid hormones was increased to normal ranges. Tests were positive for anti-adrenal antibody and anti-gastric antibody.
The complication of chronic thyroiditis, an autoimmune disease, and a positive finding for every antibody suggested the possible involvement of autoimmunity in the mechanism of development of idiopathic hypoparathyroidism. The administration of 1α-OH-D3 resulted in an increase in the serum calcium level and also normalization of levels of TSH and thyroid hormones. Thus, it is likely that the elevation of the calcium ion or immunoregulation by active vitamin D may have induced the increase in thyroid hormone secretion.抄録全体を表示PDF形式でダウンロード (1004K) -
平井 雅晴, 大屋 栄一, 宮永 健, 斎藤 八千代, 岸田 繁, 宮保 進, 中井 継彦1991 年 67 巻 12 号 p. 1345-1355
発行日: 1991/12/20
公開日: 2012/09/24
ジャーナル フリーEndothelin 3 (ET3) is a member of the novel vasoconstrictive peptide family, identified in the porcine central nervous system. The effect of ET3 on the hypothalamic-pituitary-adrenal axis in male rats was examined in vivo and in vitro.
Intravenous bolus injection of 1000pmol/kg of ET3 in free moving rats caused significant increases in plasma ACTH and corticosterone levels, almost equivalent to those of 100pmol/kg of rat corticotropin-releasing hormone (rCRH). Since an iv bolus injection of ET3 1000pmol/kg did not cause significant changes in the blood pressure of anesthetized rats or the locomotor activity of free moving rats, it seems unlikely that ET3 1000pmol/kg acted as a nonspecific stressor. When ET3 (10-11>10-7M) was added to cultured anterior pituitary cells, neither direct stimulation of ACTH release nor potentiation of rCRH action was noted.
Although it has been shown that ET3 administered systemically probably does not cross the brain-blood-barrier, circulating ET3 may reach the brain tissues through regions lacking the tight barrier, circumventricular structures. The next studies included pretreatment of antagonists or blockers of ACTH stimulating hormones to elucidate the mechanisms of ET3 induced ACTH release. The action of ET3 was virtually abolished by pretreatment of CRH-antagonist α helical CRH (150μg/rat icv). But pretreatment of catecholamine-blocker a methyl-tyrosine (100mg/kg iv), arginin vasopressin-antagonist dP-thy (Me) AVP (50 μg/rat iv) and prostaglandin-blocker indomethacin (3mg/rat iv) did not inhibit the action of ET3.
The results indicate that ET3 may play the role of a neuropeptide and that the stimulation of the CRH-neurons is mainly responsible for activation of ACTH and corticosterone release.抄録全体を表示PDF形式でダウンロード (1031K)
- |<
- <
- 1
- >
- >|