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69 巻, 6 号
選択された号の論文の7件中1～7を表示しています
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檜垣 實男, 荻原 俊男1993 年 69 巻 6 号 p. 575-587
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーThe renin-angiotensin system plays an important role in the regulation of blood pressure. Recent progress in molecular biology enables us to investigate the molecular mechanism of essential hypertension. Accumulating data suggest that the disorder of the renin-angiotensin system is not essentially a primary factor of hypertension, but it apparently plays an indispensable role in the pathogenesis of hypertension. Many researchers including us have shown that the renin-angiotensin system also contributes the development of various hypertension-related organ damages by its hypertrophic, vasculotoxic or unknown mechanisms. Gene diagnosis and newly developed antihypertensive drug therapy including gene therapeutics will open the new era of individualized and etiology-based hypertension treatment.抄録全体を表示PDF形式でダウンロード (1762K) -
山本 蒔子, 吉田 克巳, 米沢 健三, 水柿 道直1993 年 69 巻 6 号 p. 588-593
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーHypothyroid patients are usually treated with in an oral administration of 1-T4. When oral administration is impossible because of esophageal stenosis or unconsciousness in such patients, parenteral replacement is required.
We prepared a solution of 1-T4 50 μg/ml for intravenous administration in Tohoku University Hospital as follows: 5mg of 1-T4 (Nakarai Co.) was dissolved in 1ml of 0.1N NaOH and diluted with 0.9% NaCl and sterilized with membrane filter.
Two patients with esophageal stenosis due to thyroid cancer and esophageal cancer and one in a coma due to adrenal crisis had replacement with intravenous 1-T4. Initial doses of 12.5 μg or 25 μg diluted with 100ml of 0.9% NaCl containing 1% albumin were given by drip infusion. Every week 12.5 μg or 25 μg of 1-T4 was increased. By replacement of low doses, 50 μg or 75 μg, serum T4 reached a normal level. No adverse action was observed during or after parenteral replacement.
The preparation of 1-T4 was stable at least for one year and 11 months.
Parenteral replacement of T4 is a useful and safe method for patients who are unable to take drugs orally.抄録全体を表示PDF形式でダウンロード (485K) -
第III報: 耐糖能障害モデルにおける効果の検討松岡 瑛, 若林 茂1993 年 69 巻 6 号 p. 594-608
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーRecently developed, Indigestible Dextrin (PF-C) is a low viscosity, water-soluble dietary fiber obtained by heating and enzyme-treatment of potato starch. It has an average molecular weight of 1600. Results from methylation analysis via gas chromatography show the indigestible portion to be a dextrin composed of α-1, 4, α-1, 6, β-1, 2, β-1, 3, and β-1, 6 glucosidic bonds and 1, 6-anhydro-β-D-glucose (levoglucosan) as part of the reducing terminal.
Physiological attributes such as an improvement in sucrose tolerance and a reduction in blood lipid levels have since been demonstrated. In this study to establish a dose response for PF-C on blood glucose and insulin levels following a sucrose load, administration studies were conducted on normal rats and rats with impaired glucose tolerance. The results are summarized as follows:
1) To estimate an effective dose of PF-C on the reduction in blood glucose and insulin levels following an oral sucrose load, an oral sucrose (1.5g/kg body weight) tolerance test was conducted on rats. The increase in both plasma glucose and insulin levels following a sucrose + PF-C (0.075, 0.15, 0.60, and 1.5g/kg body weight) load was significantly lower compared to the sucrose load. The results show that the most effective dose of PF-C was found to be 0.15g/kg body weight.
2) Another sucrose tolerance test was conducted on three different rat model groups with drug or diet induced impaired glucose tolerance. Impaired glucose tolerance was achieved by injecting one group with streptozotocin at 1.5 days (60mg/kg body weight); a second group was injected at seven weeks (30mg/kg body weight), and a third group was fed on a high (65%) sucrose diet. For this sucrose tolerance test, the adult (7-week) streptozotocin induced diabetic rats and the high-sucrose diet rats on concurrent administrations of PF-C (0.15g/kg body weight) showed decreases in both plasma glucose and insulin levels following a sucrose (1.5g/kg body weight) load. For the neonatal (1.5-day) streptozotocin induced diabetic rat group, reduced increases in plasma glucose were observed with no change in insulin levels as a result of concurrent administration of PF-C following a sucrose load.
3) To evaluate the effect of continuous administration of PF-C on glucose tolerance, 5 week old neonatal (1.5-day) streptozotocin induced diabetic rats were kept for 4 weeks on tap water or a 10% sucrose solution with or without 1% admixed PF-C. Plasma glucose levels following a glucose (1.5g/kg body weight) load were found higher in the tap water alone group. However, plasma glucose levels were significantly reduced in the PF-C administered rats. Further, the percentage of whole body fat was reduced in the PF-C administered group compared to that of the tap water alone group.抄録全体を表示PDF形式でダウンロード (1570K) -
-臨床的および実験的検討-木越 俊和, 金子 美保子, 中野 茂, 小豆沢 定秀, 内田 健三, 森本 真平1993 年 69 巻 6 号 p. 609-620
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーResponses of plasma aldosterone (PA) to α-ACTH-(1-24) (250 μg, im) injection and graded angiotensin II (AII) infusions (2, 4 and 8ng/kg/min for 30 min at each dose) on a constant sodium intake (170mEq daily) were assessed in 17 patients with Basedow's disease and 13 age-matched normal subjects. Aldosterone production in response to ACTH, AII and potassium in adrenal zona glomerulosa cells from L-thyroxine-induced hyperthyroid rats (H-rats) were also examined. Basal levels of plasma renin activity (PRA) and urinary aldosterone excretion were significantly higher (p<0.01 and p<0.05, respectively) in the patients with Basedow's disease than in the normal subjects, whereas basal PA level was similar in the two groups. The ACTH injection induced similar increases in plasma cortisol, plasma 18-hydroxycorticosterone (18-OHB) and PA in the two groups. The graded AII infusions also produced increases in plasma 18-OHB and PA in the two groups. Responses of these two corticosteroids to AII were, however, significantly lower (p<0.05) in the patients with Basedow' s disease than in the normal subjects. In the experimental animal study, basal PRA levels and the adrenal glomerulosa cell count/adrenal were significantly higher (p<0.05) in the H-rats than in the control rats, whereas basal PA levels were similar in the two groups. Aldosterone production in response to AII, ACTH, and potassium increased in a dose-dependent manner in the two groups. Responses of aldosterone production to AII were, however, significantly lower (p<0.05) in the H-rats than in the control rats. These results suggest that the impaired responsiveness of adrenal zona glomerulosa cells to AII, as well as an increased metabolic clearance rate of aldosterone, may be involved in the abnormal aldosterone metabolism in hyperthyroidism.抄録全体を表示PDF形式でダウンロード (1055K) -
高橋 正人, 荒尾 孝, 宮地 幸隆1993 年 69 巻 6 号 p. 621-631
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーIt is known that some athletes use androgenic anabolic steroids to promote skeletal muscular strength and muscle hypertrophy in response to muscle strength training. However, it is not defined whether anabolic steroids are effective for muscle hypertrophy. In order to make this question clear, we examined the conditions of a binding assay of cytosolic androgen receptor on rat skeletal muscle as the first step. We used 3H-mibolerone as the ligand for specific binding to the androgen receptor. The following conditions of the assay were examined in this study: the incubation temperature and time, concentration of 3H-mibolerone, the effects of monothioglycerol and triamcinolone acetonide, and the effect of repeated freezing and thawing of the samples on binding. It was a suitable condition for an androgen receptor assay that samples prepared from unfrozen fresh muscle were incubated with 16nM 3H-mibolerone at 4°C for 20hrs, with monothioglycerol (20mM) and triamcinolone acetonide (25μM) added. Using these assay conditions, we measured the binding capacity of androgen receptor in rat skeletal muscles. The values of Kd and Bmax in M. gastrocnemius were 3.61±0.60×10-9M and 3.18±2.23fmol/mg protein respectively.抄録全体を表示PDF形式でダウンロード (954K) -
稲葉 宗通, 片山 茂裕, 赤羽 重樹, 丸野 世志子, 板橋 明, 石井 淳1993 年 69 巻 6 号 p. 632-635
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーWith iodinated vasopressin analogue, d(CH2)5[Tyr(Me)2, Tyr (NH2)9] AVP, at position 9, followed by purification by HPLC (specific activity 473-543Ci/mmol), a specific binding was observed in the rat liver plasma membrane fraction. Scatchard analysis indicated a single class of high-affinity binding sites with a Kd of 0.23nM and Bmax of 142fmol/mg protein. V2-agonist, DDAVP, did not displace 125I-vasopressin analogue. These results suggest that125I-d(CH2)5[Tyr(Me)2, Tyr (NH2)9] AVP with a high specific activity is a useful tool to investigate V1-receptors.抄録全体を表示PDF形式でダウンロード (325K) -
岡本 高宏, 小原 孝男, 伊藤 悠基夫, 泉雄 勝, 山下 共行, 金地 嘉春, 田中 礼子, 飯原 雅季1993 年 69 巻 6 号 p. 636-640
発行日: 1993/06/20
公開日: 2012/09/24
ジャーナル フリーThe differential white blood cell (WBC) count often reveals relative lymphopenia in Cushing's syndrome and may be a clue to the discovery of the ailment. However, the incidence of this finding has rarely been reported in the literature. We conducted a study on 40 patients with Cushing's syndrome due to adrenocortical adenoma to evaluate the diagnostic implications of relative lymphopenia.
Total WBC count, differential WBC count, basal level of plasma cortisol, urinary excretion of free cortisol and thyroid function were evaluated preoperatively. We also investigated the differential WBC count in 40 patients with thyroid tumors matched for age and sex with the Cushing's syndrome patients. The proportion of lymphocytes among WBCs was also compared between the two groups.
The proportion of lymphocytes among WBCs was significantly lower in the patients with Cushing's syndrome (19.4±10.8%) than in those with thyroid tumors (42.3±9.5%, mean±SD, p<0.05). The incidence of relative lymphopenia was high (82.5%) as well as that of increased urinary excretion of free cortisol (85.3%) in Cushing's syndrome patients. The low T3 syndrome was frequently seen (73.9%), whereas the incidences of leukocytosis and an increased level of basal plasma cortisol were relatively low (42.5% and 47.5%, respectively).
Relative lymphopenia provides useful information for diagnosing Cushing's syndrome since it has high sensitivity although it should be kept in mind that its specificity is low.抄録全体を表示PDF形式でダウンロード (428K)
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