Amino acid composition, terminal amino acid residues and carbohydrate components were analyzed for purified β-parotin. Seventeen amino acids, except methionine comparing with parotin, were found in β-parotin and N-terminal amino acid was aspartic acid. C-terminal amino acids of β-parotin were alanine and serine, and, β-parotin was considered to be a glycoprotein containing hexose and hexosamine.
Qualitatively, no difference between active and inactive forms of β-parotin was found out in the amino acid and carbohydrate composition.

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A by-product, which was obtained from β-parotin fraction, was compared with parotin in chemical properties; amino acid composition, N-terminal residue, a content of carbohydrate and isoelectric point.
From the results of these investigations, it was supposed that this substance might be parotin contaminating in the β-parotin fraction.

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Beta parotin was found to be more stable in acid solution than parotin, and equally or slightly unstable in alkaline solution; it was fairly stable between pH 2.0 and 10.0 below 40°C.
Ca-activity of β-parotin was inactivated by nitrous acid, formaldehyde, cysteine and thioglycollic acid, but not by iodine. It was not influenced either such denaturing reagents as urea and guanidine.
Ca-activity of β-parotin was destroyed after the treatment with proteases for to 24hrs. at 38°C, while L-activity was stable.

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It has been confirmed that the copper-induced ovulation is inhibited eventually by the barbiturate, just as the picrotoxin induced one. This fact would be enough to verify the nervous mechanism exerted by copper molecule, after being transmitted to the hypothalmic area essentially by a. vertebralis. The functional close link between a. vertebralis and the hypothalamus is indicated by the predominant effectiveness of the intravertebral injection of copper in eliciting ovulation. The significance of this vessel concerning the steroid control of the pituitary in the reproductive cycle was discussed.

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By means of the modified HCG augumentation method of Steelman-Pohley for FSH and the modified ovarian ascorbic acid depletion method of Parlow for LH, FSH and LH content of the pooled anterior pituitaries from the same groups were simultaneously determined. The results were as follows:
1. In mature female rats, a significant, progressive increase was found in both FSH and LH content after gonadectomy, accompanied by the appearance of castration cells.
2. Most of the post-partum ovulations in this study were found to occur between the night of Day-1 and the morning of Day-2, by examination of vaginal smears and histological findings of the ovaries.(Day-0-arturition).
A dramatic fall in LH content from a higher. level in late pregnancy to a significantly lower value on Day-1 may be interpreted as providing the LH release into the circulation responsible for postpartum ovulation.
On the other hand, FSH content did not show a significant decline until Day-2. The significance of the time lapse between the change in LH and FSH content appears very interesting but requires further studies for an appropriate explanation.
3. FSH and LH content of the pituitary during lactation after post-partum Day-3 did no have significantly different concentrations from those in cyclic rats.
The marked decrease in ovarian weight and the histological findings of ovarian suppression indicate lower FSH and LH levels in the circulation.
These results suggest that the production and release of FSH and LH are inhibited during lactation, probably by the suckling process.

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The various biological responses which were observed by the intravenous injection of SPA were now studied by the oral administration of SPA.
About 10% reduction of serum calcium level was elucidated with the dose of 2 to 5mg per kg of body weight, however, the number of the circulating leucocyteswas not affected by this treatment.
The promotion of the calcification in the dentine of rabbit incisor was also observed by the oral administration of 0.5mg per kg of body weight of SPA.
The potent uro-parotin was excreted in the urine for short period after the oral administration of a considerable amount of SPA in the sialoadenectomized rabbits.
From these results, it was confirmed that the oral administration of the adequate doses of SPA causes the various biological functions which have been found with the parenteral administration of SPA.

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The effect of the oral administration of saliva-parotin-A to rats was studied in comparing with that of subcutaneous injection of the substance. Sialoadenectomized and sham operated male rats of Donryu strain (28 days old) were orally administered with 0.6 and 3.0mg per 100g of body weight of saliva-parotin-A for 3 weeks and subcutaneously with 0.03mg per 100g of body weight. The morphologicaleffects upon tibial epiphysis and adrenal cortex were examined. The reduction of the width of cartilage plate and atrophic pattern of primary spongiosa were found in the tibial epiphysis in control non-injected rats 3 weeks after the sialoadenectomy. Subcutaneous injection of saliva-parotin-A retarded the subsequent involution to the sialoadenectomy, and the oral administration of salivaparotin-A also could retard the epiphyseal involution with the dose of 3mg per 100g of body weight, corresponding the 100 times dosage to the subcutaneous injection. No effect on the involution was recognized in rats with the oral administration of 0.6mg. In the adrenal cortex, a kind of stressful picture was observed after the sialoadenectomy or subcutaneous injection of saliva-parotin-A. In the rats received 3mg of saliva-parotin-A orally, however, such changes were neither observed in adrenal of the sialoadenectomized rats nor in the sham operated ones.

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The effects of parathyroid hormone on serum calcium and alkaline phosphatase in the chicken were studied. The injection of parathyroid extract (Eli Lilly) at the dosage level of 500 U. S. P. units per kg body weight into 13-week-old pullets significantly increased serum calcium concentration 4 hrs. after the injection, but did not change the level of serum alkaline phosphatase. The injection of 100 U. S. P. units parathyroid extract per kg body weight into 13-week-old cockerels and pullets induced changes neither in serum calcium level nor in serum alkaline phosphatase level.
The injection of the suspension of desiccated beef parathyroid powder at the dosage levels of 25 to 50 U.S.P.units per kg body weight into 13-week-old cockerels had no effect on serum calcium concentration, but significantly reduced the level of serum alkaline phosphatase. The injection of 0.5g mash diet suspended in distilled water into 13-week-old cockerels induced a significant reduction of the serum level of alkaline phosphatase.
These results indicate that the parathyroid hormone itself has no effect on serum level of alkaline phosphatase, and the detrimental effects such as the injection of unabsorbable insoluble materials reduce the level in the chicken.

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