Endocrinologia Japonica
Online ISSN : 2185-6370
Print ISSN : 0013-7219
ISSN-L : 0013-7219
Volume 27, Issue 4
Displaying 1-20 of 20 articles from this issue
  • SATORU SUEOKA, SHIGERU MATSUKURA, HIROKI YOSHIMI, MAKOTO YOKOTA, YUKIO ...
    1980 Volume 27 Issue 4 Pages 405-410
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    High molecular weight forms of immunoreactive ACTH (IR-ACTH) were studied in a human pituitary gland and 4 ectopic ACTH-producing tumors in man. Both the pituitary and tumor extracts contained “big” IR-ACTH, which eluted near the void volume, and a small amount of “intermediate” IR-ACTH components, which eluted between the void volume and 125I-αh1-39ACTH, in addition to “little” ACTH which coeluted with 125I-αh1-39ACTH by Sephadex G-100 gel filtration. A significant amount of the “big” IR-ACTH applied bound to the concanavalin A-agarose column and was eluted with 0.2M α-methyl-D-mannopyranoside, indicating the glycoprotein content of “big” IR-ACTH fractions. When the “big” and “intermediate” fractions were further analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they were resolved into 4 molecular forms of IR-ACTH with apparent molecular weights of 37, 000, 24, 000, 18, 000 and 4, 500, respectively.
    These results indicate that 3 high molecular forms of IR-ACTH are present in the human pituitary and the ectopic ACTH-producing tumors.
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  • MITSUO INADA, MARIKO OISHI, MITSUSHIGE NISHIKAWA, SHUNICHIRO KURATA, H ...
    1980 Volume 27 Issue 4 Pages 411-415
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The glycosylated hemoglobin levels were determined in the hemolysates obtained from 14 normal subjects and 67 patients with adult-onset diabetes mellitus, using the column chromatographic procedure of Trivelli et al. The levels of hemoglobin A1a+b (HbA1a+b) and HbA1c and the sum of HbA1a+b and HbA1c (HbA1a+b+HbA1c) in normal subjects averaged 2.3±0.4 (SD)%, 5.3±0.8% and 7.6±1.0%, respectively. Although a slight increase in HbA1a+b was found in patients with diabetes mellitus (mean±SD=2.8±0.7%), it was not significantly different from that in normal subjects. Despite the wide range, HbA1c and HbA1a+b+HbA1c were significantly increased in patients with diabetes mellitus (6.9±1.8% for HbA1c, p<0.01 and 9.7±2.2% for HbA1a+b+HbA1c, p<0.01). A significant correlation existed between the glycosylated hemoglobins and plasma glucose levels determined in the same blood (r=+0.57, p<0.001). Moreover, the glycosylated hemoglobin levels correlated significantly with the average glucose levels for several months preceding the hemoglobin measurements. In particular, a striking correlation was evident in the plots of HbA1a+b+HbA1c against the mean plasma glucose for 3 months prior to the hemoglobin measurements, which had a correlation coefficient of 0.79 (p<0.001). The present findings revealed that the glycosylated hemoglobins reflect the time-averaged blood glucose levels in diabetics for during approximately the proceding 3 months, indicating the usefulness of measuring the glycosylated hemoglobins in assessing the long-term blood glucose control in diabetics.
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  • HARUKA SASAKI, TERUKAZU KAWASAKI, MAKOTO OKUMURA, TAKASHI ASANO, KEISH ...
    1980 Volume 27 Issue 4 Pages 417-429
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    In two patients with Bartter's syndrome, significant increases in serum immunoreactive prostaglandin E like-material (iPGE) and urinary kallikrein excretion were observed during the control period. Urinary PGE excretion was not always increased as serum iPGE. After an administration of indomethacin, an inhibitor of prostaglandins (PG's) synthetase, their clinical symptoms, hyperactivity of renin-angiotensinaldosterone (R-A-A) system and resistence to the pressor effects of angiotensin II and norepinephrine were apparently eliminated, but hypokalemia was not corrected completely. Indomethacin caused a remarkable decline in urinary kallikrein excretion and in serum and urinary PG's values. Spironolactone also produced a significant fall in urinary kallikrein excretion in spite of an extreme increase in the R-A-A system activity. However, spironolactone did not suppress the urinary PGE excretion. After an administration of aminoglutethimide, the urinary kallikrein excretion decreased gradually, followed by a decline in aldosterone production.Short-term administration of aspirin, ibuprofen, aminoglutethimide and dexamethasone did not improve hypokalemia despite the normalization of aldosterone production.
    An angiotensin II antagonist, [Sar1, Ile8] angiotensin II, lowered the blood pressure of hyperreninemic patients. After indomethacin, a significant improvement in blood pressure response to this antagonist was observed. These results indicate that angiotenin II receptors of arteriolar smooth muscle in patients with Bartter's syndrome are still responsive to endogenous angiotensin II, andthat the R-A-A system plays a considerable role in the regulation of blood pressure. Moreover, our observations suggest that a more “proximal” cause of Bartter's syndrome is renal potassium wasting i. e., hypokalemia which causes an inappropriate production of PG's in the kidney and vascular walls, resulting in vasocontrictor insensitivity and hyperactivity of the R-A-A system. Likewise, it appears that hyperactivity of the renal kallikrein-kinin system does not stimulate the production of PG's in this syndrome, but rather is secondary to increased PG's synthesis
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  • SHIGERU KANEKO
    1980 Volume 27 Issue 4 Pages 431-438
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Progesterone (P), 20α-hydroxypregn-4-en-3-one (20α-OHP), prolactin (PRL), LH and FSH in peripheral blood plasma were measured during the early stages of phenobarbital-induced pseudopregnancy (PHB-PSP; Furudate, 1975) and the changes in these hormone levels were compared with those during the 5-day estrous cycle and early stages of ordinary pseudopregnancy (PSP) induced by cervical stimulation.
    During the cycle, surging releases of LH, FSH and PRL, occurred in the afternoon of the first day of vaginal estrus (E1) and levels of these hormones were low during the other stages. Two large rises in P were observed ; one in the afternoon of day E1 and the other during 2 days of vaginal diestrus (D1 and D2). 20α-OHP showed daily diurnal rises sometime between 1600 and 2300 hr.
    A phenobarbital (PHB) injection (100mg/kg, sc) at 1900 hr on D1 initiated daily PRL surges, which were similar to those observed following cervical stimulation though of less magnitude, without altering the levels of LH and FSH significantly. Continued elevation of P and lowering of 20α-OHP levels were observed as during the ordinary PSP.
    Continued vaginal diestrus of PHB-PSP was blocked by simultaneous injections of bromoergocryptine (CB-154; 1mg, sc) and PHB. In addition, the PSP-like vaginal diestrus was evident in all the rats receiving PRL administration twice daily for 3 consecutive days, starting at 2100 hr on D1. Therefore, PRL surges induced by PHB administration will be solely responsible for the luteal functionalization in PHB-PSP rats.
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  • YASUO HARUKI, KANJI SEIKI, YOSHIO IMANISHI
    1980 Volume 27 Issue 4 Pages 439-446
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Using a highly potent synthetic progestin, R5020 (17α, 21-dimethyl-19-nor-4, 9-pregnadiene-3, 20-dione), the characterization of progestin binding components was attempted in cytosols from the anterior pituitary and various brain tissues of castrated or castrated-adrenalectomized male rats. In both types of operated rats, estrogen administration increased the number of binding sites of R5020 in the anterior pituitary and hypothalamus-preoptic area, but not in the cerebral cortex, amygdala-hippocampus nor in the cerebellum. After incubation of the anterior pituitary cytosol with [3H]-R5020 with or without various unlabeled steroids, a specific progestin binding was found in the 7-8S region with a binding capacity of 250fmoles/mg protein and equilibrium dissociation constant of 8nM. A specific progestin binding was also found in the cytosol from hypothalamus-preoptic area sedimenting in the 7 S region with a binding capacity of 150 fmoles/mg protein and a dissociation constant of 6 nM. Specific binding components in these tissues were heat-labile and protein in nature. Little or no 7S binding was found in the other brain tissues. Although 4-5S binding in the cerebral cortex and 4 S bind ng in the amygdala-hippocampus and cerebellum were found, binding specificity in those tissues was low.
    It was concluded that the pituitary gland and hypothalamus-preoptic area of male rats contained progestin receptor in their cytoplasms.
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  • TOSHIO TANAKA, TSUYOSHI YAMAGUCHI, KANJI KUMA, HIROYOSHI HIDAKA
    1980 Volume 27 Issue 4 Pages 447-450
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The effect of propranolol on plasma dopamine-beta-hydroxylase activity and triiodothyronine was investigated in twelve subjects with Graves' disease. Plasma DBH activity, serum triiodothyronine and thyroxine were determined beforehand and electrocardiograms were recorded on the same days as the blood samples were taken. Propranolol administration was associated with clinical amelioration in blood pressure and ECG. There was a positive and significant correlation between changes in DBH activity and triiodothyronine caused by propranolol.
    These data offer evidence that there may be an interaction between plasma DBH activity and triiodothyronine during propranolol administration in hyperthyroidism.
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  • KEIICHI KAMIJO, AKIMITSU SAITO, AKIRA YACHI, TAKEO WADA
    1980 Volume 27 Issue 4 Pages 451-455
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Plasma GH responses to TRH were investigated in 50 cancer patients. Out of 50 cancer patients, 33 were found to be responders. At 15, 30 and 60 min after TRH injection, the mean values GH for cancer patients was significantly higher than the corresponding value for normal controls and the base-line level following saline injection in cancer patients (p<0.001).
    Our results suggests that a possible alteration of hypothalamic-pituitary function regulating GH secretion may occur in patients with cancer.
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  • EIKO HARA, MASAYUKI SAITO
    1980 Volume 27 Issue 4 Pages 457-461
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    A method for studying the enteroinsular axis of rats under conditions comparable to those in experiments on human subjects was developed by chronically implanting an oral tube and two cardiac catheters into rats. These implantations were easy to perform and made it possible to give glucose and other stimulatory agents orally and/or intravenously and to take sequential blood samples without anesthetizing the rats or distrubing their movements. In this way, it was possible to confirm that oral loads of glucose and fat potentiate the insulin response induced by hyperglycemia.
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  • YING-SHIH LEE, YASUO AKANUMA, YASUHIKO IWAMOTO, KINORI KOSAKA, NOBUSAD ...
    1980 Volume 27 Issue 4 Pages 463-469
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Plasma, β-N-acetylglucosaminidase (NAG) activity was measured in streptozotocin diabetic rats, in non-diabetic rats during starvation and refeeding, and in diabetic patients and normal subjects. The enzyme activity increased significantly in the diabetic rats and in the starved non-diabetic rats. The activity decreased markedly after insulin injection in diabetic rats and after refeeding in non-diabetic rats. The plasma NAG activity (532±24nmole/hr/ml, M±SE) in 21 diabetics with FBS value less than 100mg/dl was higher than the enzyme activity (455±15) in 42 normal subjects (p<0.01), supporting the idea that a more intensive treatment is necessary to normalize the metabolic derangement of diabetes. There was no significant difference between diabetics with and without microangiopathy. While the influence of age and abnormal liver function was noted, there was no correlation between the NAG activity and the platelet adhesiveness or between the NAG activity and the plasma triglyceride. The results suggest that the plasma NAG activity increases in diabetes mellitus, either with microangiopathy or not. The reason for the failure to demonstrate a significant correlation between the total plasma NAG activity and microangiopathy is discussed. The analysis of the subfractionation of the plasma NAG may be necessary to disclose a significant correlation with microangiopathy.
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  • NORIMICHI KOONO
    1980 Volume 27 Issue 4 Pages 471-476
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Serum concentrations of thyroxine (T4), 3, 5, 3'-triiodothyronine (T3), 3, 3', 5'-triiodothyronine (reverse T3, rT3), thyronine-binding globulin (TBG), thyroid stimulating hormone (TSH) and cortisol in summer and in winter were compared in carefully selected normal adult men (aged 30-49 yr); 107 people from Sapporo (58 in summer and 49 in winter) and 15 hospital workers. A modest but significantly higher serum T3 concentration was recorded in winter than in summer, while T4, TSH, TBG and cortisol levels were similar in both seasons. Serum rT3 concentration in the Sapporo subjects was slightly higher in summer than in winter but difference was not statistically significant. However, a paired analysis of rT3 concentration in the hospital workers had significantly higher levels in summer than in winter, showed a significant rise in the rT3 to T3 ratio in summer compared to winter. These results were not likely to be due to changes in TBG, cortisol and hematocrit values. The present findings and our previous report on a similar TSH response to thyrotropin-releasing hormone in summer and in winter, indicate that the changes in thyroid hormone metabolism rather than in thyroidal secretion would, at least partly, be responsible for the reciprocal alteration in T3 and rT3 concentrations.
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  • HIROSHI WAKIMOTO, SUSUMU TAKAYASU, KEISHI MATSUMOTO
    1980 Volume 27 Issue 4 Pages 477-482
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Specific dihydrotestosterone (DHT) binding was demonstrated in the whole cell sonicates obtained from serially subcultured human endometrial fibroblasts. This binding showed a high affinity and specificity for DHT (Kd=3.3×10-10M). Various kinds of excess nonradioactive steroids were arranged, as a result of competition among them, in the following decreasing order of binding affinity: DHT, testosterone, cyproterone acetate, 17β-estradiol, progesterone, and 5α-androstane-3α, 17β-diol. Androsterone, 5α-androstanedione, cortisol, androstenedione and estrone hardly affected the binding even at 200 fold excess. Saturation analysis indicated that cultured endometrial and genital skin fibroblasts have a similar number of binding sites for DHT (14.3±8.5 vs 11.5±8.6 fmoles/mg cell protein). Sucrose density gradient centrifugation brought about a sharp peak of 3H-DHT in the 4S region in high ionic strength buffer. This binding was abolished by heating sonicates at 37°C for 1 hr, treating them with pronase or adding to them excess nonradioactive DHT. Endometrial fibroblasts also contained testosterone binding components which formed a peak in the 4S region and showed a similar binding affinity to that for DHT.
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  • SHOGO ICHII
    1980 Volume 27 Issue 4 Pages 483-493
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The effect of the administration of androgens (testosterone and dihydrotestosterone), antiandrogens (progesterone and cyproterone acetate) and anabolic steroids (Bolandiol, Nandrolone, Furazabol and Methenolone) on depletion and replenishment of the androgen receptor in the cytosol of ventral prostate from castrated rats was examined using 3H-R1881 as the ligand. Administration of androgens lead to a rapid fall in the level of the receptor and the receptor in the cytosol was replenished in the period following treatment. Length of the period of receptor depletion was dependent on the dose of androgen administered. Administration of antiandrogens did not cause any depletion of the receptor and concomitant administration of testosterone induced a pattern similar to that of the depletion observed following administration of testosterone alone. Effect of pretreatment of animals with ethidium bromide, an intercalating dye which has been shown to prevent the in vitro nuclear binding of steroid hormone-receptor complexes, on the depletion of receptor induced by testosterone administration was also insignificant. Anabolic steroids depleted the receptor but the degree of depletion was relatively low. The rate of inhibition of in vitro 3H-R1881 binding by these anabolic steroids was not correlated to the rate of in vivo depletion of the receptor. The significance of these observations is discussed.
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  • YOSHIMASA TASAKA, SACHIKO INOUE, KOJI MARUNO, YUKIMASA HIRATA
    1980 Volume 27 Issue 4 Pages 495-498
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Twenty-four-hour variations of plasma hPP levels together with plasma glucose, IRI and GI levels were studied in four normal human subjects. Before examination, their food intakes were strictly controlled during two days' hospitalization. Plasma hPP values were assayed by a radioimmunoassay. They showed sharp peaks after each meal. Mean levels of hPP just prior to breakfast were 88±26 pg/ml (SEM), and the peak values of the plasma hPP one hour after each meal were 307±124 pg/ml, 398±77 pg/ml, and 544±118pg/ml, respectively. During the nocturnal sleeping period the hPP levels were stable and returned to the fasting level of the previous morning. IRI increased significantly after each meal, but no significant change was found in plasma GI levels.
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  • Effect of Dorsal Deafferentation of the Preoptic Area and Hypothalamus
    KOREHITO YAMANOUCHI
    1980 Volume 27 Issue 4 Pages 499-504
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Daily injections of 500μg testosterone propionate (TP) for 21days induced mounting behavior in all of 10 castrated male rats and the mean mount frequency (MF) for 30min was 32.4±6.6 (mean±S. E.). The same treatment with TP induced mounting behavior in 6 out of 8 ovariectomized rats (female control), but the mean MF was 2.8±0.7. Eight out of 12 ovariectomized rats which received a horizontal half-circle cut anterior to the anterior commissure (anterior roof deafferentation, ARD) with an L-shaped Halasz knife displayed mounting behavior more frequently and their MF was 14.5±5.1. The latent period between the introduction of a receptive female and the occurrence of the first mounting (mount latency, ML) in ARD rats was significantly shorter than that of the female controls. A half circle cut located posterior to the anterior commissure (posterior roof deafferentation, PRD) and sham deafferentation (SD) in the female rats had no effect on MF and ML.
    On the day following the last test for male behavior, all female rats were injected with 0.5mg progesterone in order to examine female sexual behavior. The mean lordosis quotient (LQ) of the ARD group was 62.5±13.4. In the female control, SD and PRD groups, however, LQs were significantly lower than that of ARD rats, being 15.1±10.9, 37.5±14.7 and 23.8±11.8, respectively. These results suggest that the dorsal inputs to the POA and hypothalamus which pass anterior to the anterior commissure may exert an inhibitory influence not only on the female sexual behavior mediating system but also on the mounting mediating system in female rats.
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  • YOICHI IZUMI, MASANOBU HONDA, MAKOTO TSUCHIYA, YASUHISA UEDA, MICHINOB ...
    1980 Volume 27 Issue 4 Pages 505-511
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The effects of somatostatin on plasma renin activity (PRA) and blood pressure were evaluated in patients with essential hypertension (EH) and in normotensive subjects. All subjects examined were hospitalized and placed on a diet containing 7-8g/day sodium chloride and received an intravenous infusion of somatostatin (500μg/20ml of saline, for 60 min) in the basal condition. During somatostatin infusion, the mean blood pressure (MBP) remained unaffected in all patients with EH and the normotensive subjects, while the PRA decreased slightly in the EH group. When the patients with EH were classified according to their renin levels (low, normal and high), parallel significant decreases in MBP and PRA were found only in the high renin group during the somatostatin infusion. No significant change in MBP and PRA was observed in the other groups including the normotensive subjects.
    To assess the activity of synthetic somatostatin, the plasma levels of growth hormone (GH) and cyclic AMP were measured. These levels were lowered significantly during the infusion and the GH levels showed a rebound 15 min after cessation of the infusion. The cyclic AMP returned to the basal levels, but no rebound was observed.
    The above data indicate that the fall in blood pressure in the high renin group in the basal condition was probably due in part to reduced renin release by somatostatin, and the maintenance of high blood pressure especially in high renin EH.
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  • JINU-HAUNG SU, TAKESHI ASO, TORO MOTOHASHI, HIDEKI AOCHI, MASATERU MAT ...
    1980 Volume 27 Issue 4 Pages 513-520
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Double antibody radioimmunoassay methods were developed for the determination of baboon luteinizing hormone (bLH) and follicle stimulating hormone (bFSH). The bLH radioimmunoassay employs a unique anti-ovine LH serum (GDN-15) and ovine LH (LER-1056-C2) for radioiodination, while the bFSH radioimmunoassay employs an heterologous system, i.e., an anti-ovine FSH serum (H-31) and purified human FSH for radioiodination. The reference standard used in both assays is a crude rhesus pituitary extract (LER-1909-2).
    Elevated endogeneous baboon plasma TSH and prolactin induced by the intravenous administration of 500μg of TRH had no influence on the levels of LH and FSH, whereas simultaneous intravenous administration of 100μg LHRH and 500μg TRH raised the levels of LH and FSH in plasma. hPRL, hCG, hTSH and hGH did not cross react with either the bLH or the bFSH assay system.
    The determination of plasma LH and FSH concentrations in daily samples from 6 mature female baboons throughout ovulatory menstrual cycles revealed patterns qualitatively similar to those of the rhesus monkey and human females.
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  • TOSHIO FUJINO, HIROSHI KATO, SABURO YAMASHITA, SHOJIRO ARAMAKI, HITOSH ...
    1980 Volume 27 Issue 4 Pages 521-525
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Effects of domperidone, a dopaminergic antagonist, on serum prolactin levels were studied in 6 normal men and 6 normal cyclic women at the different phases of their menstrual cycles (i.e., the follicular, the preovulatory and the luteal phases). Domperidone (10 mg, i.v.) caused significant increases in serum prolactin in all cases within 15 min after the injection. The prolactin response was significantly (p<0.01) higher in women than in men, and there was no significant difference in the prolactin responses among the three phases of the menstrual cycles. These results indicate that domperidone may be an effective stimulator of serum prolactin secretion in human beings.
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  • TAKAKO NAKAZAWA, KIYOJI TANAKA, YOSHIAKI OKADA, YUICHI KUMAHARA
    1980 Volume 27 Issue 4 Pages 527-532
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Serum factors which stimulate DNA synthesis were investigated in patients with anorexia nervosa. The factors were assayed by examining [3H]-thymidine incorporation into the nuclei of human fibroblasts in culture using autoradiography. The mean percentage of labeled nuclei (labeling index) induced by serum was significantly lower (p<0.005) in 5 patients with anorexia nervosa (21.0±5.9 percent, mean±S. D.) than in 5 normal adult subjects (33.8±3.3 percent). Twoof the patients with anorexia nervosa were treated with intravenous hyperalimentation (IVH). In one of the IVH treated patients, the labeling index increased from 17.4to 32.8 percent at the 6th week of treatment, and in the other increased from 19.4 to 29.8 percent at the 4th week. In both patients, body weight increased by 1.5 to 2kg. IVH in these patients increased DNA-synthesis-stimulating activity.
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  • SEIICHIRO KAWASHIMA, TAKAO MORI, TAKEJI KIMURA, YASUMASA ARAI, YASUAKI ...
    1980 Volume 27 Issue 4 Pages 533-539
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Female mice of B6AF1 hybrids (C57BL/6N×A/HeN) were treated neonatally with 5 daily injections of 20μg estradio1-17β or sesame oil from the day of birth. About half of them were ovariectomized at 40 days of age, and the other were given an implantation of an estradiol-17β pellet under the dorsal skin at 80 days of age. The pellet was removed 30 days later. Vaginal smear records showed persistent vaginal cornification in a number of neonatally estrogenized mice regardless of ovariectomy. When killed at 16-21 months or at 24-32 months of age, hyperplastic lesions ranging histologically from those resembling squamous cell carcinomas to adenosislike structures were observed in neonatally estrogenized mice. The development of lesions was more pronounced in mice killed at 24-32 months of age than at 16-21 months. Secondary estrogen treatment when adults did not appreciably affect the promotion of lesions. It is therefore evident that neonatal exposure to high doses of estrogen was important primarily in connection with the neoplastic changes in the cervicovaginal tract.
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  • 1980 Volume 27 Issue 4 Pages e1
    Published: 1980
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
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