Effects of adrenergic blocking agents, dihydroergotamine, dihydroergotoxine (Hydergine), priscoline and dibenamine, on intravenous glucose tolerance curve were investigated with normal fasting rats.After the pretreatment with dihydroergotamine, dihydroergotoxine and priscoline the type of glucose tolerance curve was transformed into exponential.Dibenamine did not demonstrate such an action. At the same time, the inhibiting effect of these agents on adrenaline hyperglycemia was investigated. There were not any evidences that dibenamine inhibited adrenaline hyperglycemia, while the other 3 agents could depress it. And it was concluded that the atypical blood sugar curve after intravenous glucose administration was produced by overlapping of the humped curve produced by transitory hyperglycemia due to stimulation of sympathetic nervous system and the “true” glucose tolerance curve. The latter could be separated from the former by means of the pretreatment with dihydroergotamine, dihydroergotoxine and priscoline

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Parotin and S-parotin were adsorbed largely on the acid alumina and slightly on the neutral alumina. In contrast with parotin and S-parotin, trypsin was adsorbed on the neutral alumina and not completely adsorbed on the acid alumina. Chymotrypsin was also not adsorbed on the acid alumina as same as trypsin. Thus the acid alumina column chromatography was found to be useful for the purification of parotin and S-parotin.
The pH 4.5-precipitate of parotin-digest by trypsin showed the adsorption affinity on alumina column similarly as parotin. Parotin-T. A, the biologically active fraction in the somewhat degradated pH 4.5-precipitate, was isolated in homogeneous state by the column chromatography on acid alumina. The aqueous solution of parotin-T. A showed the ultraviolet absorption maximum at 275.5 to 276mμ of wave length. From the ultracentrifugal measurements, it was estimated that the sedimentation coefficient to be 3.16×10^-13, the diffusion coefficient to be 3.10×10^-7, and the molecular weight to be 99, 000. The amino acid component was found to be the following 16 kinds; alanine, arginine, aspartic acid, cystine, glutamic acid, glycine, histidine, leucine (and/or isoleucine), lysine, phenylalanine, praline, serine, threonine, tryptophan, tyrosine and valine.
The N-terminal amino acids were identified as aspartic acid and glycine.
The author would like to express his deep gratitude to Prof. Y. Ito of this institute for his kind advice during the course of this work. Thanks are also due to Mr. Y. Kubota, Miss M. Takahashi, Mrs. F. Suzuki and Mrs. H. Ogawara for their kind cooperation and advice for this work.

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The active leukocyte-increasing fractions prepared by tryptic digestion of parotin were separated by fractional precipitation with trichloroacetic acid, and two active peptides, parotin-T. B₁ and parotin-T. B₂ were isolated by the acid alumina column chromatography. Both peptides were estimated as to be homogeneous by the paper electrophoresis.Parotin-T. B₁ showed the calcium activity and the leukocyte increasing response. Parotin-T. B₂ showed only the leukocyte increasing response.
Some properties of parotin-T. B₁ and parotin-T.B₂ were described and also compared with those of parotin and parotin-T. A.
Parotin-T. B₁ and parotin-T. B₂ showed same ultraviolet absorption maximum at 275mμ of wave length. The amino acids components of both peptides were shown to be the same following 14 kinds; alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, leucine (and/or isoleucine), lysine, proline, serine, threonine, tryptophan, tyrosine and valine. The N-terminal amino acid of these peptides were estimated as aspartic acid.
The activity of sensitization against parotin was recognized in parotin-T. A and parotin-T. B₁ by the method using the uterus of guinea pig sensitized by parotin previously. But in parotin-T. B₂ it was lacking.

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Saliva-parotm-A injected intravenously in rabbits caused highly significant decrease in serum citric acid 24 hrs. after the injection.A linear relationship was observed between the percentile decrease in serum citric acid and the logdosage of saliva-parotin-A. Effects on serum calcium, phosphorus and protein were also observed. However, the effect on citric acid was most remarkable.

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An active parotin-like substance was obtained with acetone or ethanol fractionation procedure from bovine crude parotin and its recovery was almost 70%.
In the alumina and XE-58 column chromatography of the reprecipitate at 50% acetone concentration in acetone fractionation, 2-2.5-fold activity (0.25-0.2mg/kg) and recovery of 50-70% were obtained but not homogeneous state.
The reprecipitate at 50% acetone concentration was further purified with acrinol and ammonium sulfate.The purified substance having hypocalcemic activity at the dose of 0.1mg/kg, was obtained with a yield of 10-12mg/kg bovine parotid gland. The purified substance was almost homogeneous in electrophoretic and ultracentrifugal analyses.This purified parotin-like substance was named α-parotin.

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By means of fractionation procedure with acetone, acrinol and ammonium sulfate, a-parotin was isolated in a homogeneous state from crude parotin. Activity of α-parotin was 0.1mg/kg, and the yield was 10-12mg/kg bovine parotid gland.α-Yarotin is considered to be a glycoprotein and its isoelectric point is pH 3.4-3.6. It consists of 17 amino acids and scarcely shows maximum ultraviolet absorption.α-Parotin is also distinctly different in the various properties from parotin.

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Results were obtained to support the view that the presence of intact pituitary gland must be a prerequisite to the adrenal ascorbic acid depletion after administration of serotonin into rats. Possibility of the direct stimulative action of serotonin on adrenal cortex was not supported, though not disapproved of, under the condition of the present experiment.

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