Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
46 巻 , 4 号
選択された号の論文の13件中1~13を表示しています
Original
  • Masakazu HASEGAWA, Yuzuru KURABAYASHI, Toshinori ISHII, Kazuya YOSHIDA ...
    原稿種別: Original
    1997 年 46 巻 4 号 p. 251-257
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    Recently, a forced-air-ventilated micro-isolation system (FVMIS) has been recognized to accurately maintain microenvironmental conditions inside cages, but the details of the relationship between the concentrations of carbon dioxide (CO2) and oxygen (O2) and the air change rate inside the cages have never been reported. In this study, the proper intra-cage air change rate was examined based on the CO2 concentration and O2 concentration inside the cages measured by changing the ventilation volume inside the closed cages of the FVMIS while housing animals. In the experiments, three 8-week-old Wistar strain male rats weighing 303 g on average were housed in each FVMIS cage (capacity: 0.0223 m3), and the temperature, relative humidity, CO2 concentration and O2 concentration were measured when the air change rate inside the cages was varied from 10 air changes per hour (ACH) to 120 ACH. It proved that the CO2 concentration in the FVMIS cages decreased uniformly with the increase in the air change rate. As a result, 60 ACH was required to maintain the CO2 concentration level inside the FVMIS cages equivalent to or less than that in the conventional housing. Otherwise, when based on the O2 concentration, 50 ACH was required. In consideration of these results and others based on ventilation, airflow, temperature and the ammonia concentration reported previously, we concluded that the proper air change rate inside the FVMIS cages should be approximately 60 ACH.
  • Michio SUZUKI, Koji UETSUKA, Masami SUZUKI, Junko SHINOZUKA, Hiroyuki ...
    原稿種別: Original
    1997 年 46 巻 4 号 p. 259-267
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    We performed immunohistochemical examinations on type II collagen-induced arthritis (CIA) mice, focusing attention on the changes in distribution of plasma proteins and extracellular matrix materials (ECM) and in expression of adhesion molecules. The limb joints of male DBA/1J mice immunized with bovine type II collagen were obtained at 6 to 20 weeks after the first immunization. In the early stage of CIA, deposition of fibrin, IgG, von Willebrand factor (vWF) and fibronectin was detected on the surface of the synovial lining layer and articular cartilage and in the articular cavity. In the stage of pannus formation, prominent proliferation of ICAM-1-positive capillaries and marked infiltration of LFA-1-positive neutrophils were observed in the pannus. The superficial portion of the pannus and basement membranes of proliferated capillaries were strongly positive for type IV collagen and laminin. In the late stage, the pannus invaded and destroyed articular cartilage and subchondral bone, and strongly positive immunostainabilities for both lysozyme and fibronectin were observed on the surface of the pannus and at the junctional portion between the pannus and the cartilage. The present immunohistochemical findings on the distribution of plasma proteins and ECM materials and the expression of adhesion molecules in CIA mice were similar to those in rheumatoid arthritis (RA) in many aspects. This suggests that CIA is a useful model for the investigetion of RA.
  • Satoko SUGIYAMA, Kosaku FUJIWARA
    原稿種別: Original
    1997 年 46 巻 4 号 p. 269-277
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    The angles related to the coxal joints were comparatively studied in four-limbed walking animals and two-limbed ones including man and birds. Between animals with both types of walking, no significant difference was observed in the neck-shaft angles (NSA), which was equivalent to the acetabulum angles (ACA) at the connection of the femoral head with the acetabulum. The anteversion angles (AVA) were equivalent to the horizontal ACA. Canine species showed two different forms of the femoral neck with or without modification by the femoral AVA, probably being breed-specific and nutrition-dependent. In the narrow-striped wallaby as well as avian species, the femoral head showed a postversion with a minus-version angle for lifting the body axis in the frontal and upward direction to hold the whole body weight on the hind-limbs, in particular at the anterior part of the acetabulum. In man, the connection between the femur and acetabulum greatly varied among individuals, possibly according to differences in the life style.
  • Ryoichi OHTSUKA, Yasufumi SHUTO, Hideaki FUJIE, Makio TAKEDA, Takanori ...
    原稿種別: Original
    1997 年 46 巻 4 号 p. 279-286
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    BN rats are well-known for their high capacity for IgE production and hyperresponsiveness to exposure to allergens or other chemicals. We examined the histological changes in the nasal cavity, trachea and lungs of BN and F344 rats after the inhalation of aerosol formaldehyde (HCHO), which exerts direct toxic effects on the respiratory system. The incidence of clinical signs such as sneezing and abnormal respiration in HCHO-treated F344 rats was higher than that in HCHO-treated BN rats. The mean body weight of HCHO-treated F344 rats apparently decreased in comparison with control F344 rats, but that of HCHO-treated BN rats was not significantly different from that of control BN rats. Changes such as squamous metaplasia, stratification, degeneration and desquamation were observed by light microscopy in nasal, tracheal and bronchial mucosa in the lungs of the HCHO-treated F344 rats. In the HCHO-treated BN rats, similar but milder lesions were restricted to the nasal mucosa. Scanning electron microscopic observation supported these light microscopic observations. These results suggest that BN rats have lower sensitivity to HCHO inhalation than F344 rats.
  • Hooi Hoon ANG, Meng Kwoon SIM
    原稿種別: Original
    1997 年 46 巻 4 号 p. 287-290
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    The effects of Eurycoma longifolia Jack were studied on the libido of sexually experienced male rats after dosing them with 200, 400 and 800 mg/kg body weight twice daily of different fractions of E. longifolia Jack for 10 days. Results showed that E. longifolia Jack produced a dose-dependent increase in mounting frequency of the treated animals with 400 mg/kg of chloroform, methanol, water and butanol fractions resulting in mounting frequencies of 5.3 ± 1.2, 4.9 ± 0.7, 4.8 ± 0.7 and 5.2 ± 0.1, and 800 mg/kg further increased them to 5.4 ± 0.8, 5.4 ± 0.8, 5.2 ± 0.6 and 5.3 ± 0.2 respectively but there were no erections, intromissions, ejaculations or seminal emissions during the 20-min observation period which allowed for the measurement of sexual arousal reflected by mounting frequency uninfluenced by other behavioural components. This study provides evidence that E. longifolia Jack is a potent stimulator of sexual arousal in sexually vigorous male rats in the absence of feedback from genital sensation.
  • Tetsuya MATSUZAKI, Kanae MATSUZAKI, Minesuke YOKOYAMA, Muneo SAITO
    原稿種別: Original
    1997 年 46 巻 4 号 p. 291-296
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    The period of ovulation in mature house musk shrews was examined in a natural mating group and a superovulation group treated with gonadotropin. In the natural mating group, ovulation started 14 hr after mating in 3 of the 7 house musk shrews (42.8%), and occurred in all 5 house musk shrews by 15 hr after mating. In the superovulation group, ovulation started 13 hr after the administration of hCG in 3 of the 5 house musk shrews, and was observed in all 5 shrews by 16 hr after the administration. In the natural mating group, ovulated ova were collected from the ovarian bursa of 14 house musk shrews 14-20 hr after mating (mean, 2.2 ± 1.0 ova) and from the oviduct of 42 animals 14-24 hr after mating (mean, 3.6 ± 1.8 ova). Among the ova ovulated 14-16 hr after mating, both mature ova with the first polar body and immature ova without the first polar body were observed. In the superovulation group, ovulated ova were collected from the ovarian bursa of 31 house musk shrews 13-22 hr after the administration of hCG (mean, 9.7 ± 6.8 ova), and from the oviduct of 28 animals 13-24 hr after the administration of hCG (mean, 20.0 ± 11.7 ova). There were also mature and immature ova in the ova ovulated 13-16 hr after the administration of hCG. The time when ova ceased to be recovered from the ovarian bursa roughly coincided with the time when new corpora lutea ceased to be found in the ovaries. These findings suggested that the period of ovulation of house musk shrews was 14-20 hr after mating in the natural mating group and 13-22 hr after the administration of hCG in the superovulation group. Both the natural mating group and superovulation group ovulated both mature ova with the first polar body and immature ova without the first polar body.
  • Ei SAKAGUCHI, Hiroshi ITOH, Tsuyoshi KOHNO, Seinosuke OHSHIMA, Kiyoshi ...
    原稿種別: Original
    1997 年 46 巻 4 号 p. 297-302
    発行日: 1997年
    公開日: 2003/12/23
    ジャーナル フリー
    The effects of different fiber sources on feed intake, weight gain and digestibility of fiber were examined in guinea-pigs fed pelleted diets containing alfalfa meal, oaten hay, beet pulp and commercial hay cubes mixed with a basal diet at ratios of 3:1 (75% in the diet), 1:1 (50%) and 1:3 (25%). The basal diet contained 50.0% corn, 4.1% wheat, 22.1% wheat flour and 17.7% corn gluten meal. Food intake increased as the amount of fiber source was increased, but not in the case of beet pulp. The most digestible fiber (ADF and NDF) was that of beet pulp. Apparent digestibility of dry matter decreased with increasing ratios of fiber source to the basal diet for all fiber sources, but fiber and crude protein digestibilities varied and depended not only on the ratio of fiber to the basal diet but also on the source of the fiber.
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