EXPERIMENTAL ANIMALS
Online ISSN : 1884-4170
Print ISSN : 0007-5124
ISSN-L : 0007-5124
Volume 7, Issue 6
Displaying 1-5 of 5 articles from this issue
  • A. TAKATA
    1958 Volume 7 Issue 6 Pages 167-175
    Published: November 30, 1958
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    1. L'auteur a décrit la morphologie comparative de Syphacia obvelata et d'Aspiculuris tetraptera.
    2. L'infection deS. obvelatas'établit chez les souris qui ont pris des oeufs pondus autou de l'anus et contenants déjà des larvae tandis quo cello deA. tetrapterase rèalise par l'ingestion des oeufs développés on quelques jours dane l'extérieur après avoir été rejetés avec des féces. La derniére ressemble plutôt à l'infection orale d'Ascaris ou de Trichuris.
    3. Les oeufs infectants deS. obvelataont été obtenus chez lee souris de quatre semaines en utilisant le Scotch tape adhéré autour de l'anus et ils ont été collectionnés et cultivés en solution de Ringer à 37°C. En cas deAspiculuris tetrapterales oeufs provenus des femelles adultes ont été mis sur la cellophane trempée avec lo formol à 0.5% et soutenues à 27°C on 5 à 7 jours afin d'obtenir les oeufs contenants des larvae.
    4. L'espace de temps entre l'nfection et Ia réjection d'oeufs s'étrle de 11 à 16 jours apré le contact naturel en ces deS. obvelata, cependant qu'elle atteint 23 jours aprés l'infection orale artificielle en cas deA. tetraptera.
    5. L'auteur a confirmé aussi la ponte périanale deS. obvelata, essayé une méthode triplée de l'inspection anale pour examiner Ia quantité des oeufs selon les parties périanales aussi bien que la condition de leur ponne.
    6. Quant à la résistance des oeufs, ceux deA. tetrapterapeuvent développer mêmo dans le formol à 10% et produire leurs larvae.
    7. Los souris âgées de quatre semaines sont lee plus sensibles àS. obvelatatandis que lee souris plus jeunes ou plus âgées sont moms sensibles à cette espèce de vers. En cas deA. tetrapteerales animaux plus plus âgées manifestent la sensibilité plus élevée au point de vue de l'examen des fèces.
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  • Eitaro WADA
    1958 Volume 7 Issue 6 Pages 176-181
    Published: November 30, 1958
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    There have been opinions that mouse is less suitable for the assay of tetanus toxoid. The author has tried to reinvestigate the mouse-assay-method by selecting the mouse strain and improving the experimental conditions. Among various mouse strains used, gpc proved to be most suitable for assay. The immunizability of dd strain varied greatly depending on the season of the year. The immunizability of dd was almost similar as that of gpc in summer, while the former was significantly inferior to the latter in winter. The extent of the variation in dd strain was larger than the standard error of ID50in each experiment, while that of gpc remained in the scope of experimental error calculated from each experiment during the whole experimental period of 46 months. Such variations in immunizability sometime inf iuenced greatly the relative potency of two tetanus toxoid, -i. e. the value measured by two mouse strains showed a significant difference in winter time.
    The reason of such variation could not be ascribed to the low room temperature in winter. Some of the possible reasons were discussed.
    In conclusion, dd was inadequate in the potency test of tetanus toxoid and it was possible to get the reliable and reproducible results of assay by using gpc strain.
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  • NOBUO KATO
    1958 Volume 7 Issue 6 Pages 181-187
    Published: November 30, 1958
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    To examine the reproducibility of the works of TABEYI et al., sensitivity of male ddN mice to the infection withSal. euteriticlisand to the injection with acetylcholine was tested. Mice were divided into two groups after examination of their blood pictures. The one group contained mice of which leucocytes pictures showed one of TABEYI's indexes for sensitivity; namely myelocyte, metamyelocyte, more than 3.5 per cent eosinophilic leucocyte, more than 10 per cent monocyte and plasma cell. The other was those which did not show the indexes. LD50of acetylcholine inject-ed intravenously was 17.4mg per kg body weight in the former group and 13.5mg in the latter. When 10-4mg ofSal. enteritidisS64 were inoculated intraperitoneally, mortality was 8/10 (periods of death 6-12 days) in the former group and 9/10 (periods off death 6-9 days) in the latter. The results did not agree with the opinion of TABEYI. Based on the results it was stated that his opinion might have errorneous points.
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  • 1958 Volume 7 Issue 6 Pages 187-190
    Published: November 30, 1958
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
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  • [in Japanese]
    1958 Volume 7 Issue 6 Pages 191-192
    Published: November 30, 1958
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
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