Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 0007-5124
Volume 33, Issue 4
Displaying 1-12 of 12 articles from this issue
  • Kyoko MATSUI, Kazuya ANDO
    1984 Volume 33 Issue 4 Pages 465-469
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    Antiataxic mechanisms were investigated in Rolling mouse Nagoya (RMN) . The present study was to elucidate the influence of dopaminergic (pimozide, apomorphine) and cholinergic (atropine, physostigmine) drugs on the antiataxic effect of TRH. The degree of ataxic gait and spontaneous motor activities in RMN were measured by the open field method and ANIMEX-II Pretreatment with pimozide and apomorphine had no influence on the antiataxic effects of TRH, while pretreatment with physostigmine suppressed these effects and in contrast, with atropine, increased then. The increase of spontaneous motor activities after TRH injection was antagonized by pretreatment with pimozide and physostigmine, but accentuated by pretreatment with atropine. These results may indicate that the antiataxic effects of TRH are, at least partially, mediated by the cholinergic mechanism.
    Download PDF (545K)
  • Takashi YAMADA, Hideo UCHIDA, Tsutomu NISHIYAMA
    1984 Volume 33 Issue 4 Pages 471-479
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    We studied the effect of selective reproduction on rats purchased from a breeder, so as to detect learning impairments in their offspring. The parent rats selected for mating were categorized as bright or dull in terms of learning ability on the basis of a learning test in a water maze or passive avoidance response (PAR) . Offspring were tested in the same way as the selected rats, with their responses being compared for each generation. 1. The matings, consisting of bright × bright, bright×dull, dull×bright, dull×dull, resulted in a significant increase in the number of dull offspring of dull×dull from parents selected by water maze or PAR learning test. 2. Bright or dull mothers selected by PAR learning were treated with chlorpromazine 6 mg/kg (2 times/ day/s.c.) on the 13th day of gestation. Afterwards, a slight increase in dull offspring of dull parents was seen. 3. These results indicate that in order to evaluate the learning ability of F1 in a reproductive test, it is first necessary to check the reaction of the parents, purchased F0, and to classifiy them as bright or dull.
    Download PDF (918K)
  • Itaru MIYAMOTO, Sumi NAGASE
    1984 Volume 33 Issue 4 Pages 481-485
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    Age-related changes in the molecular weight of hyaluronic acid from the skin of rats was studied using fetuses, newborn animals and rats 1, 2, 3, 4, 13, 26 and 52 weeks of age. Average values of the molecular weight of hyaluronic acid varied with age in three phases: 39×104 in fetuses and up to the age of one week, 55×104 in individuals aged to 26 weeks and 87×104 in individuals 52 weeks of age. The distribution of moleuclar weight was quite different between 4 weeks and 52 weeks of age. At 4 weeks of age, the molecular weight of hyaluronic acid was widely distributed, ranging from 18×104 to 79×104, while that from 52-week-old individuals was very high with a distribution ranging from 92×104 to 119×104.
    Download PDF (518K)
  • Syusaku SUZUKI, Akio AGO, Shiro MOHRI, Hayao NISHINAKAGAWA, Junichi OT ...
    1984 Volume 33 Issue 4 Pages 487-496
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    The mandibular gland of the Djungarian hamster was examined by light microscopy, and transmission and scanning electron microscopies. Its acinar cells reacted with periodic acid-Schiff (PAS) and were weakly stained with alcian blue (AB) . There were intercellular canaliculi between the acinar cells. These cells therefore appeared to be seromucous. The acinar epithelium was composed of light cells containing various spherical secretory granules. The granular cells of the mandibular gland possessed many acidophilic granules exhibiting a positive reaction to PAS stain. They were frequently observed at the junction of the acini and intercalated ducts in all mandibular glands examined. All of these cells were light and contained secretory granules of varying size and density. The intercalated ducts consisted exclusively of light cells possessing a few round granules of high density in the apical region. The striated ducts were comprised of two portions—a secretory portion and a typical striated portion without secretory granules. The secretory portion consisted of light, dark and specifically light epithelial cells containing acidophilic granules, which exhibited a strongly positive PAS reaction. The epithelium of typically striated portions was composed of light and dark cells containing fine vacuoles in the apical region. The mandibular gland of the Djungarian hamster revealed no histological differences between sexes.
    Download PDF (7345K)
  • Satosi KAGABU
    1984 Volume 33 Issue 4 Pages 497-499
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    A new treatment for inducing superovulation in adult rats was examined. Virgin Wistar-Imamichi rats at 11 weeks of age were pretreated with PMSG on the metestrus day. Then, the animals were anesthetized with sodium pentobarbital on the expected estrus day. The maximum number of ova shed in the animals thus treated was 100 with a combination of 40 I. U. of PMSG and hCG. If the treatment with sodium pentobarbital was omitted, the maximum number remained at 43 with different combinations of PMSG and hCG dosages.
    Download PDF (299K)
  • Toru SATO, Kajuro KOMEDA, Kazuhiko SHIRAMA
    1984 Volume 33 Issue 4 Pages 501-508
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    From vaginal smear and histological observations, Chinese hamster (Cricetulus griseus) exhibited four day estrous cycles and had a gestation period of 20 days. The concentrations of progesterone fluctuated in a biphasic pattern during the estrous cycle with peak values at diestrus and proestrus. The levels of the hormone increased approximately threefold from day five to day eight of gestation, remained elevated until parturition, and then fell precipitously during lactation to levels on day zero of gestation.
    Download PDF (4684K)
  • Masamichi KUROHMARU, Tetsu HAYAKAWA, Makoto SEKI, Katuya ZYO
    1984 Volume 33 Issue 4 Pages 509-518
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    The intestinal mucosa of the pika was examined with the naked eyes, a light microscope and a scanning electron microscope and was compared with that of the rabbit. The duodenal mucosa of the rabbit showed wavy folds different from so-called villi, while that of the pika exhibited leaf-like or columnar villi. In addition to the specific ileocecal lymphoid apparatuses, the pika had the peculiar region between the cecum and the proximal colon. That region called“the constricted portion”possessed characteristic net-arranged folds and welldeveloped muscular layers. At the lateral surface of these folds, small villuslike protrusions projected into the lumen in large numbers. The spiral fold ran around the mucosal surface of the rabbit cecum, whereas numerous slender protrusions, cecal digitations, projected into the lumen of the pika cecum. Although the colon of the pika showed a similar external figure to that of the rabbit, some differences were obviously found in histological structures. The first segment of the Pika proximal colon with three teniae possessed several protrusions and well-developed mucous glands, while that of the rabbit had neither protrusions nor mucous glands. The second segment of the Pika proximal colon with one tenia was covered with numerous villus-like protrusions, while that of the rabbit was composed of wart-like protrusions. The tubular mucous glands were observed in the lamina propria of the pika as well as the rabbit. The distal colon of the pika showed a flat mucosal surface and possessed tubular mucous glands as observed in the rabbit.
    Download PDF (6027K)
  • Shosaku HATTORI, Kazuya YAMANOUCHI
    1984 Volume 33 Issue 4 Pages 519-524
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    The Watase's shrew (Crocidura horsfieldi watasei) which is a native in the Nansei Islands in Japan, is one of the smallest mammalian species with 5-8 g of the body weight. Both males and females were caught in the field of the Amami Islands, and their internal organs in the thoracic and abdominal cavities were examined under the dissecting microscope and by the serial cross sections method. In comparison with the musk shrew belonging to the same subfamily Crocidurinae, the following features were basically similar; very short intestine in comparison with the body length, the absence of cecum, and the structure of the genital organs. Besides, the followings were different; the small sized body, the small sized spleen compared with the body weight, and the cardiac glands limitted in the end of the esophagus.
    Download PDF (745K)
  • Hiroyoshi NINOMIYA, Tohru FUKASE, Tsunenori NAKAMURA
    1984 Volume 33 Issue 4 Pages 525-528
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    Using scanning electron microscopy of celluloid impressions the feline penile spines in the adult and after castration were studied. A solution of celluloid in a mixture of amyl acetate was placed on the glans. The replica thus obtained was coated with gold in an ion-spatter coater, and observed under a scanning electron microscope (JSM-1) . The structure of the penile spines and fine arrangement of superficial cells of the epidermis could be observed threedimensionally. Since the penile spines are the only known external indicators of the level of male hormone in cats, and since their fine structure can be observed without excising the penile skin, this simple method can be applied for diagnosis of fertility of the male cat.
    Download PDF (1817K)
  • Satoru KOZIMA, Masahide KATO
    1984 Volume 33 Issue 4 Pages 529-533
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    The influence of two weeks of repeated oscillation stress on rats was investigated. Organ weights, liver glycogen, adrenal ascorbic acid, hematological and serum biochemical analysis and maximum contraction of vas deferens induced by noradrenaline were measured. In stress-loaded rats, body weight fell to 85-90% compared with control rats. Atrophy of the thymus and hypertrophy of the adrenals were found in stress-loaded rats. Hematological and serum biochemical findings revealed that white blood cells and blood glucose decreased, but NEFA increased significantly. Serum sodium increased, but potassium decreased. Maximum contraction of vas deferens induced by noradrenaline was potentiated in stress-loaded rats. The other findings did not differ from those of controls. From these results, it is suggested that the stress-loaded rat shows some abnormalities, but may adapt partially to stress.
    Download PDF (466K)
  • Yoshihisa KUBOTA, Yuji YAMADA, Sentaro TAKAHASHI, Osamu MATSUOKA
    1984 Volume 33 Issue 4 Pages 535-538
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    The present study was carried out to develop the inhalation system possible to measure the respiratory volume in rats during the inhalation. The conventional body plethysmography was confirmed to be able to measure the respiratory volume of anesthetized rats accurately. Consequently, this method made it possible to measure the respiratory volume of rats during the inhalation studies by using animal restraint holders combined with the inhalation chamber as body plethysmograph boxes. The practical availability of this inhalation system was proved in the inhalation studies of latex particles, in which the number of particles inhaled by each rat could be estimated by measuring the air concentration of latex particles as well as the respiratory volume of each animal.
    Download PDF (431K)
  • Yoshibumi MATSUSHIMA, Tadaharu IMAI, Osamu WATANABE, Hironori KAWAHARA ...
    1984 Volume 33 Issue 4 Pages 539-542
    Published: October 01, 1984
    Released on J-STAGE: August 25, 2010
    JOURNAL FREE ACCESS
    Large numbers of spontaneously occurring polypoid or slightly elevated lesions were observed in the tongue, mainly the dorsum linguae near the margo linguae, of DBA mice. Histologically the lesion consisted of granulation tissue with focal calcification, and involved superficially the tongue muscle. Often the calcareous deposits were encircled by multinuclear giant cells. The frequency of the calcified tongue lesion was high in lines of DBA/2 (DBA/2NCrj, DBA/2NJcl and DBA/2J), and DBA/1 (DBA/1Jcl and DBA/1J) ; the SM/J, BALB/c, C57BL/6 and C3H/He strains did not have the lesion. Among hybrid mice, CDF1, a hybrid of DBA/2 and BALB/c, a few had the lesions but no BDF1 mice, a hybrid of DBA/2 and C57BL/6, had any. The frequency was high in the hybrids of DBA/1 and SM/J. These results seem to indicate that the occurence of the tongue calcified lesions was controlled by polygene.
    Download PDF (2781K)
feedback
Top