Non-invasive treatments for constipation of healthy subjects (56 female university students:18.3 average years of age) were conducted by daily taking in the pickled vegetables for 2 weeks. The subjects were divided equally in two groups for taking pickles containing gamma-aminobutyric acid after fermented with Lactobacillus brevis or non-fermented pickles as placebo. Not great differences in blood fatty acids were observed between two groups. However, the groups fed with fermented pickles had not atypical presentation of fecal impactions and frustrating fecal incontinence was attenuated.