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Volume 103 (1994)
- Issue 6 Pages 245-
- Issue 5 Pages 193-
- Issue 4 Pages 141-
- Issue 3 Pages 83-
- Issue 2 Pages 43-
- Issue 1 Pages 1-
Volume 103, Issue 2
Displaying 1-4 of 4 articles from this issue
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Masahiro KAWAMURA, Takashi MATSUI1994 Volume 103 Issue 2 Pages 43-48
Published: 1994
Released on J-STAGE: February 06, 2007
JOURNAL FREE ACCESSThe isolated bovine adrenocortical cells are prepared aseptically by the use of collagenase and deoxyribonuclease. The isolated cells are suspended in Ham F-10 medium containing 5% fetal calf serum, 10% newborn calf serum, 2.5% horse serum and antibiotics. The seeded cells are cultured at 37°C in a humidified atmosphere of 5% CO2 in air. Steroidogenic activity for ACTH reached the maximum in the 2 to 3-day primary cultured cells; the maximum response to ACTH in these cells is more intense than that in freshly isolated bovine adrenocortical cells. The primary cultured cells have prostaglandin, muscarinic, ATP and β-adrenergic receptors that are linked to steroidogenesis in addition to ACTH and aldosterone receptors. Thus primary cultured bovine adrenocortical cells are a useful tool to study these receptors and the intracellular events that are associated with the receptors. We also demonstrated that the fura 2 loaded primary cultured monolayer cells on glass cover slips provide us much more information than suspended cells in the study of intracellular Ca2+ mobilization in adrenocortical cells.View full abstractDownload PDF (10538K) -
Kazuhiko YANAI, Takehiko WATANABE1994 Volume 103 Issue 2 Pages 49-58
Published: 1994
Released on J-STAGE: February 06, 2007
JOURNAL FREE ACCESSVarious brain imaging techniques have become available in the past decade. These include techniques to evaluate brain structure using X-ray computerized tomography or magnetic resonance imaging and techniques to assess brain functional activities (cerebral blood flow, brain energy metabolism and brain protein synthesis) using positron emission tomography (PET). PET also makes it possible for the first time to evaluate the states of various types of neurotransmitter receptors, such as dopamine D2 and D1, serotonin 5-HT2, muscarinic cholinergic, opiate, benzodiazepine and histamine H1, and to determine the state of monoamine oxidase (A and B) under in vivo conditions. These techniques cannot only be used to map “brain neurochemistry” in normal human beings, but they will also increase our knowledge by demonstrating neurochemical abnormalities in a wide range of neurological and psychiatric disorders or those that happen during normal aging. The PET techniques may be applicable to the development of new drugs in the pharmaceutical industry. We have been exploring the methodology of using 11C-labeled antagonists for mapping functional neurotransmitter receptors in human brain directly and noninvasively by PET. The present review article provides an outline of the conceptual and methodological progress over the past several years that has made it possible to visualize neurotransmitter receptors in the living human brain by PET on the basis of our original work.View full abstractDownload PDF (18435K) -
Yasuhiro MATSUMOTO, Yutaka NOGUCHI, Jun INUI1994 Volume 103 Issue 2 Pages 59-66
Published: 1994
Released on J-STAGE: February 06, 2007
JOURNAL FREE ACCESSEffects of Y-20811, a thromboxane A2 synthetase inhibitor, on the arachidonic acid (AA)-induced responses were investigated in the isolated canine coronary artery superfused with blood from a donor dog and Krebs-Henseleit solution. When the coronary artery was superfused with Krebs-Henseleit solution, AA did not have any remarkable effect on its tone. In the coronary artery superfused with blood, in contrast, AA (10-100 μg) caused phasic constriction followed by relaxation. After denudation of the endothelium, the contractile response was augmented, and the relaxant effect was abolished. Y-20811 (1 mg/kg, i.v.), administered into the donor dog, inhibited the contraction and augmented the relaxation. Indomethacin (5 mg/kg, i.v.) and aspirin (30 mg/kg, i.v.) also inhibited the AA-induced contraction. However, indomethacin inhibited the relaxation induced by AA (10-100 μg), whereas aspirin inhibited the relaxation induced by a low dose of AA (10-30 μg). These results suggest that Y-20811 inhibits the TXA2-induced contraction and augments the production of the relaxant metabolite of AA in the coronary artery.View full abstractDownload PDF (13689K) -
Shunsuke IMASAKA, Sadao NAKAYAMA, Katsuji OGUCHI1994 Volume 103 Issue 2 Pages 67-78
Published: 1994
Released on J-STAGE: February 06, 2007
JOURNAL FREE ACCESSThe present study was performed to determine the serum lipid and lipoprotein changes in cholesterol free-high fructose diet (HFD)-fed rats and to examine the effects of clinofibrate (CF) on these changes. HFD feeding for 2 weeks increased the high density lipoprotein (HDL1) subfraction and decreased the low density lipoprotein subfraction. The contents of total cholesterol (TC), triglyceride (TG) and phospholipid (PL) in the serum increased by HFD feeding. CF inhibited the increases in HDL1 and lipid content. The continuance of HFD-induced hyperlipidemia was investigated in rats fed a normal diet after HFD feeding for 4 weeks. The HFD-induced increases of TC, PL and HDL1 continued for 5 days. TG was also increased by HFD feeding, but the elevated level was not maintained after feeding of the normal diet was initiated. The administration of CF for 3 or 5 days to the rats that had been switched to the normal diet inhibited the increases in lipids and HDL1. The increase of cholesterol synthetic activity continued for 3 days after the rats were switched to the normal diet but CF increased to a higher level than that of the HFD group. The main mechanism of the hypolipidemic effect of CF on HFD-induced hyperlipidemia is considered to be the increase of lipid excretion and HDL1 metabolism.View full abstractDownload PDF (20360K)
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