In rabbits, the intravenous injection of HN
2resulted in severe leucopenia and depression in serum cholinesterase activity, without change of electrophoretic pattern of serum. Administration of folic acid, cystein and methionine plus vitamin B
12before injecting HN
2, resulted in partial protection against the leucopenia induced by HN
2. But administration of methionine and vitamin B
12alone was less effective, even with large doses. The depression of the serum cholinesterase was related to the severity of leucopenia. One mechanism of HN2 action is inactivation of SH bearing enzymes but such mechanism is not the only possibility.
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