The composition of
cis-trans isomers of β-carotene in fresh vegetables and fruits was studied using high performance liquid chromatography (HPLC) with a photodiode array detector. All-
trans-β-carotene was the predominant β-carotene and accounted for 66-96% of total isomers, depending on the vegetables and fruits. The main
cis isomer detected in green-colored vegetables and fruits, such as spinach, broccoli, garland chrysanthemum, asparagus and kiwifruit is 9-
cis-β-carotene. In contrast, 13-
cis-β-carotene is found mainly in yellow and red colored vegetables and fruits, such as loquat, sweet potato, squash, tomato and watermelon.
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