The effects of cleaning and disinf ection on bacteria contaminated endoscopes were investigated both experimentally and clinically by assaying of the remaining bacteria with a stripping solution. In the experimental study, an endoscope was artificially exposed with either Pseudomonas aeruginosa or Mycobacterium terrae, and the external surf ace, the channels, and the valves of the endoscope were cleaned and disinfected. Cleaning alone produced the following reductions in bacterial counts : external surface, 10
5 before cleaning, l0
0-10
1 after cleaning ; suction channel 10
6-10
7 before, 10
3-10
4 after; air/water channel 10
5-10
6 before, 10
2-10
3 after ; valves, 10
5-10
6 before, 10
4-10
5 after. Completely negative results, however, were not obtained. Disinfection performed in addition to cleaning produced a virtually total elimination of residual bacteria. In 14 cases of clinical studies, cleaning performed on each of the endoscope components produced the following effects in terms of bacterial counts : external surface, including valves, 10
5-10
7 before, 10
3-10
5 after; channels, 10
6-10
9 before, 10
1-10
3 after. Disinf ection combined with cleaning produced a virtually total elimination of bacteria of endoscopes. Thus, it is concluded that sufficient degree of reduction in bacterial counts can be achieved by performing disinfection in combination with the cleaning of the endoscopes from our experiments and clinical studies.
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