Vegetative growth of white clover grown under long and short day was compared in order to make clear the cause of seasonal production of this clover, especially of low production in summer. Results of 4 experiments were reported. In the Exp. 1, treatments of long and short day were made on young plants, sown in small wooden boxes. The experiment was carried out from autumn to spring in a glasshouse. In Exp. 2, the same treatments were made on transplanted stolons in pots during the same season with Exp. 1. In Exp. 3, treatments were made on young plants the same as Exp. 1, but during in warm season, from summer to autumn. In those experiments a number of clipping was done to examine the yields of regrowth. In Exp. 4, ten treatments of different daylength were made on young plants sown in pots. Plots changing from long day to short day at four different number of days after beginning treatment were provided. Similarly four plots of changing from short day to long day were provided. Together with those plots, continuous long and short day plots were prepared as a control. The following results were obtained : 1. There was a little difference in day length responses between Ladino type white clover (Italy) and common type white clover (Nolins), as well as between young plants and a sod made up by transplanted stolons. Whereas, significant differences were found in vegetative growth between short and long day plots. 2. The long day plot produced higher green yield than short day from the beginning of treatment up to early flowering stage, however, produced lower yield after that (Fig.s 1 and 5, Tables 3 and 6). A transition of superiority in the yield from the long day plot to short day plot, corresponding with growth stage, seemed to be very much important to understand the seasonal production in the field. 3. The superiority of the long day plot in yield at the early stage was appeared due to large leaves (Fig. 6, A.B.) and high rate of leaf appearance. The superiority of the short day plot at the later stage was appeared due to a large number of leaves, a large leaf area (Table 1), a large amount of healthy root and stolon, high activity of root and high rate of leaf appearance. 4. The main reason of growth depression in the long day plot was assumed to be deterioration of the structure for regrowth, which was formed by decreased weight of storage organs, such as root and stolon and number of growing points. The structure for regrowth seems to play an important role to reproduce new leaves after clipping. Long day plants were vigorous in top growth, while poor to built up a structure for regrowth (Tables 2, 3 and 5) and this seems to give explanation for occurrence of production cycle. 5. The death and decay of roots and an old part of stolons, sometimes even a death of stumps (Fig. 6, C.D.) were observed in long day plants in an experiment of summer. Whereas, this was never observed in short day plants. The death and decay of those was due to the diseases and it was assumed that decreases of resistance to diseases occurred at the flowering stages, at which growth of roots and stolons were severely depressed. This fact was thought to be important in regard to summer depression in the field, since some evidence proved that the death and decay of roots and stolons was a direct cause of summer depression. 6. These findings were obtained from the continuous long and short day experiments. But it was indicated in the Experiment 4. that changing from short day to a long day or from a long day to a short day, which used to occur in natural condition, influenced more severely than the continuous long day or continuous short day (Fig. 8). 7. A schema of the developmental phase of Ladino clover in a year at Chiba is presented as Table 7, according to the growth response to changing daylength and the field observations reported previously. 8. Causes of summer depression is understood i
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In order to trace the yearly change of both plant numbers and tiller numbers per pant and area, and also to find out their relations to the yield with more accuracy than the previous trials, orchardgrass seedlings propagated from one original plant derived from var. Frcde, were transplanted during the fall to establish the two kinds of swards, that is the high density sward with 400 plants per 10a (HD-sward) and the low density sward (LD-sward) with 100 plants per 10a. The size of each plot was 4m^2 (2m×2m). These swards were differentially fertilized in level of nitrogen with the same amounts of other elements ; HN (40kg N per 10a), MN (20kg N) and LN (10kg N), respectively. Furthermore, two cutting systems were employed ; the 4-cut system which took the initial harvest at the early boot stage and the 3-cut system in which the first cut was taken at the maturing stage of the plant. In both systems the subsequent cuts were made when the plant reached around 50cm high. The cutting height was 5cm above ground surface. Plant height and emergence or death of tillers per plant were measured on 20 plants at one or two week intervals. 1. Generally, the height of plant and canopy increased with the increased application of nitrogen, whereas under hot and dry summer conditions high nitrogen retarded the regrowth. High density with high nitrogen depressed the elongation of the plant in the early heading stage resulting in the lowest height at the maturing stage. Plant weight was greater in the low density plant and became greater with increased application of nitrogen (Figs. 1, 2). 2. The percentage of dead plants over a year increased with the increased nitrogen application and in the high density sward, it was greater when the initial cut was taken later at the maturing stage (3-cut system) than when cut at the early boot stage (4-cut system) (Fig. 3). 3. Tillers of each plant emerged rapidly at the three periods, that is, from early spring to prior to the boot stage, after flowering to the early August and in the fall with short day-length and low temperature. The death of tillers, on the contrary, occurred at the two periods, from early head to full bloom and after cutting during hot and dry weather (Fig. 4), and the former death was caused in the younger tillers emerged April to May because they became weaker under the thick mutual shading (Table 1). The total tiller numbers per plant over a year emerged or died were always greater in the LD plants than in the HD plants. The yearly emerged numbers of the HD sward were greater in the 4-cut system (Table 2). Generally more tillers emerged with increased nitrogen application, but with the 3-cuts of the LD sward, medium nitrogen produced more tillers than high nitrogen. With the low level of nitrogen, the numbers of bath emerged and died tillers were small (Table 2). 4. In the LD sward, the number of live tillers per plant when cut 3 times reached three peaks in one season ; the first was the heading stage at the end of May, the 2nd was at the middle August after the initial cutting, and the 3rd was in the fall. When cut 4 times, the first peak was about 10 days later but the other two were at almost the same dates as with the 3-cuttings. In the HD sward, the first peak occurred 10 days earlier but the other two at almost the same dates as in the LD sward. The variability in tiller numbers was always less in the HD sward as compared with the LD sward (Fig. 6). 5. The total number of live tillers per area was greater and reached a peak earlier in the HD sward by the middle May, but decreased earlier due to the death of many tillers, as compared with the LD sward. The latter sward gradually increased in number. It reached the first peak a month later with the 4-cuttings, and half a month later in the 3-cuttings. During the summer, however, the difference between the two swards in terms of live tiller number almost disappeared. In the
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