An antigen (C. W. P.) was prepared by adding liquid paraffin to crude wax extract-ed from human tubercle bacilli (Aoyama B strain). Rats were inoculated intraperitoneally with this antigen and then challenged at 10 and 20 weeks with the murine leprosy bacillus. The effect on onset of murine leprosy was examined and the following results obtained.
1) From the standpoint of leproma development, challenge with 500-fold diluted murine leprosy bacillary suspension at 10 weeks showed that there was almost no difference compared to the control. When challenged with a 5000-fold diluted bacillary suspension, there was a slight inhibition of leproma development at 10 weeks while at 20 weeks, a considerable inhibition was found. (See Table 1, Fig. 1)
2) The weight of the leproma was less and there was some suppression of bacillary distribution to the inguinal and axillary lymphnodes in the inoculated animals. (See Table 2, Fig. 2 and Photo)
The results suggest that the crude wax of human tubercle bacilli has a onset suppres-sing action, though slight, towards murine leprosy.

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Mice were inoculated with murine leprosy bacilli (Hawaiian strain) and the protein, lipoprotein and polysaccharide fractions of the serum studied by paper electrophoresis. The following results were obtained.
1) Serum protein fraction : From about the 16 th week after infection, *-globulin increased and albumin decreased. When divided into the leproma group and the ulcer formation group this finding was more marked in the latter. In the group in which leproma could not be observed macroscopically, that is, the "spontaneous cure" group, the values were close to normal. From about 10 months after infection, an increase in a-globulin was found and there was a marked increase in the ulcer group at 12.5 months.
2) Serum lipoprotein fraction : A marked increase in a-lipoprotein was found in both liproma and ulcer groups but the pattern differed slightly between the two. This finding is present 10 months after infection.
3) Serum protein-bound polysaccharide fraction : A marked increase in *-polysac-charide was found after 7 months in the ulcer group but there was no marked increase in the leproma group.

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The knowledge and appreciation of high school students in regard to leprosy was investigated as an aid in improving the leprosy prevention program.
Five thousand questionnaires each were distributed in a relatively prevalent prefecture Gifu, and in Ibaragi Prefecture where leprosy is relatively rare. The response was 78.5%
The results show that of all the major diseases, leprosy is the most feared and this fear is extreme compared with others. The cause for the fear was emotional in the greater number (42.9%) followed by incurability of medical therapy (33.3%).
The causal factor was reported as infection in the wide sense, including predisposition by 58.0% but 25.9% believed that heredity was the cause.
Though leprosy was feared, there was no special prejudice towards leprosy patients and the majority (91.0%)were sympathetic.
Almost all reported that the proper steps would be taken if leprosy was found in members of the family or relatives but the answers were reasonless as what the individual would do if himself contracted leprosy. In answer to the question as what you would do if leprosy was discovered in the family of the person who you had selected for marriage, 58.9% answered, that the engagement would be broken. This was especially true in the case of girls.
Knowledge concerning the possibility of cure was low (11.7%)but the attitude toward patients who would be returned to society was cooperative in 45.0%, rational in 40.5%, and negative or uninterested in 12.8%
Knowledge concerning leprosy was lower in Ibaragi prefecture than in Gifu prefecture and more girls than boys still had a mistaken idea about leprosy.

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The majority of the therapeutic agents of the DDS series are those with an aromatic ring attached to 4-aminophenylsulfone and the amino radical in the 4 position is considered important from the standpoint of therapeutic effect. It is known that when the amino on both sides of DDS replaced with the acetyl radical, there is a great difference thrapeu-tically. The toxicity is reduced but at the same time, the effect should also be affected. As 4, 4' diacetaminodiphenylsulfone has been found to be effective against pneumococci and streptococci it can be assumed that it is converted to an effective form in the body. The mechanism of action was therefore investigated.
Deacetylization was first studied by extracting the urine and blood of subjects given the agent with ethyl acetate and subjecting the extract to paper electrochromatography. DDS and monoacetate were proven.
The same procedure was carried out in the DDS administered subject and monoacetate was shown to be present. Similar results were obtained with mouse liver.
The pharmacological actions of DDS, the monoacetate and the diacetate were compared. It was found that the toxicity of the latter two was far weaker than that of DDS. It was also found that DDS paralyzes the peripheral endings of the parasympathetic nerves which accelerate intestinal motility, causes a rise in blood pressure and stops the pulsation of the toad heart in systole in a dose of 20*50mg/20cc whereas the latter two agents do not have these actions. On the basis of these results, it was decided that 4, 4'-diacetaminodi-phenylsulfone could be safely used clinically and it was applied in 10 cases of leprosy. It was found that this agent was effective. It is suggested that 4, 4'-diacetaminodiphenyl-sulfone will be useful in cases in which DDS or Promin cannot be to related or in those in which there is no longer any response to Promin or DDS.

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The potency of 8 lots of Lepromin prepared at different times was tested in animals sensitized to leprosy bacilli and tubercle bacilli. The potency of the lots showed a similar trend in both groups of animals at 24 hours, and aside from a single lot, a similar trend was also observed at 48 hours.
The findings suggest that in view of the weak allergic response in leprosy bacilli sensitized animals and the difficulty in obtaining samples of leprosy bacilli, tubercle bacilli sensitized animals can be used for testing potency of Lepromin.

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1) Isonicotinoy1-3-4-diethoxybenzal hydrazone (No. 284) markedly suppresses onset of murine leprosy. When treatment is continued for 3 months (3 mg daily by mouth), it becames difficult to find acid-fast bacilli at the site of inoculation.
2) In mice infected 9 months previously with murine leprosy bacilli and showing definite lesions, administration of 5 mg of isonicotinoy1-3-4-diethoxybenzal hydrazone daily for 2∼3 months results in a macroscopic cure of the leproma or ulcer at the site of inoculation. Acid.fast bacilli, however, are still present. When treated with INH (3 mg daily per os), acid-fast bacilli were found not only at the site of inoculation but also in the liver and spleen.

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When sensitized to the tubercle bacilli or inoculated with BCG, early Lepromin reaction similar to that of Tuberculin reaction is reported to occur in the normal human, guinea pigs and rabbits.
According to Chase, normal guinea pigs attain tuberculin allergy by the passive cellular transfer from the guinea pig sensitized with tubercule bacilli. The author confirmed his study and at the same time the attitude of Lepromin reaction was investigated.
The results suggest that the ascitic cells of the guinea pig sensitized with tubercle bacilli contain a certain heat labile reaction factor in common between Tuberculin reac-tion and Lepromin reaction.

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Cycloserine was administered in a dose of 1.0 mg daily, 6 days a week for 120 days in mice inoculated with a small quantity or large quantity of murine leprosy bacilli and the effect compared to that in the untreated control.
No effect was noted in the groups inoculated with either the small or large quantity of bacteria and no difference due to the dose of cycloserine was found. In other words, there was no difference between the treated group and the untreated control. From the results, it is believed that this agent has no effect in murine leprosy.
The results differ from those of Chang, but this may be attributed to the difference in experimental methods. A universal standard screening method should be set up as soon as possible.

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