Percoll密度勾配遠心法と水相2層分配法によるDraper法(IMMLEPプロトコール1/79)とPercoll密度勾配遠心法による森法とにより精製されたらい菌について若干の生物学的活性を比較検討した。精製らい菌のintegrityは後者において前者におけるよりも良かった。上述の2つの精製法によりえられたらい菌の100°C,熱処理ワクチンの宿主マクロファージ機能調節効果について検討した。その結果,2種のワクチンは細胞を採取する21日(皮下)および4日(腹腔内)前の2回注射により宿主マクロファージの貪食および活性酸素産生能を増強したが,抗リステリア活性を増強することは出来なかった。Draper法によりえられたらい菌のワクチンは森法により作られたものよりも宿主マクロファージの貪食活性をより高めたが,その活性酸素産生能の増強の点では逆の関係がみられた。

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Intracellular killing of Mycobacterium leprae and M. lepraemurium by cloned macropha- ge cell line was observed by direct staining with FDA/EB or in the spread subcutaneous tissue preparation with Ziehl-Neelsen stain. Clone 16, derived from J774, can produce superoxide anion (O2^-) and H₂O₂ upon appropriate stimulation, while clone C3C, a variant in oxidative metabolism, defects in production of reactive oxygen intermediates. After both clones of the cells were infected with M. leprae or M. lepraemurium for three hours, and further incubat-ed in 5% CO₂-air at 37°C, most of the bacteria of both species were stained green with FDA/EB staining. Under the microscopic observation of the spread subcutaneous tissue preparation, there was no difference in number of bacteria between clone 16 and C3C cells, but decreased number of bacteria was assessed in the cells of further incubation in 5% CO₂-air at 37°C after one hour-infection than in the cells immediately after the one hour-infection periods. These results indicated that M. lepraemurium, and may be M. leprae, was partially killed or growth-inhibited by oxygen-independent killing mechanisms of macrophage cell line, rather than by reactive oxygen intermediates.

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Peripheral nerve lesions of experimental leprosy in monkeys i.e. 2 mangabey monkeys, 2 rhesus monkeys and 3 african green monkeys, were studied by electron microscopy and semi-thin section method. Sources of leprosy bacilli with which these 7 monkeys were inoculated were isolated from lepromata of mangabey monkey A015-natural infection and mangabey mon-key A022. Mangabey monkey A022 was inoculated with leprosy bacilli isolated from leprom-ata of mangabey monkey A015. Details and methods of the experimental leprosy in the monkeys were shown on Table 1.

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Histopathological finding of n. ulnaris of rhesus monkey 8664 inoculated with leprosy bacilli from lepromata of mangabey monkey A022 was studied by semithin section method.
Mangabey monkey A022 had been inoculated with leprosy bacilli from lepromata of ma-ngabey monkey A015-natural infection.
Period between date of inoculum and sacrifice of the rhesus monkey was about 2 years.

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Histopathological findings of ramus superficialis, n. peroneus of rhesus monkey A125 and ramus superficialis, n. plantaris lateralis of African green monkey 8178 were studied. These monkeys were inoculated with leprosy bacilli isolated from lepromata of mangabey monkey A015. Period between date of inoculum and date of sacrifice of the rhesus monkey was about 3 years, and in African green monkey 8178 it was about 5 years.

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N. peroneus and n. ulnaris of mangabey monkey A022 were stud, ed about the relation-ship between progress of histopathological findings and anatomical location of peripheral ner-ves. Mangabey monkey A022 was inoculated with leprosy bacilli isolated from lepromata of mangabey monkey A015. Period between date of inoculum and death of the monkey was about 4 years.

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