A naturalized plant, Lactuca serriola L. (Asteraceae) was collected from Hiroshima Prefecture, Honshu, southwestern Japan for the first time. This species was found growing by the road side at a reclamation area in Fukuyama, Mihara, Higashi-hiroshima, Hiroshima, Hatsukaichi and Otake Cities in the prefecture. Three individuals in Hiroshima and Hatsukaichi Cities including two formae, fo. serriola and fo. integrifolia, share a single haplotype of the nuclear ribosomal internal transcribed spacer (nrITS).

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