Psoriatic skin is composed of inflammatory epidermal and dermal tissues. We hypothesized that cell
to cell interactions by means of cytokine response may be altered in psoriasis. In order to define this theory, in
vitro experiments were carried out using the cell culture system. Psoriatic keratinocytes and fibroblasts were
simultaneously co-cultured on each side of the collagen membrane in the medium of high calcium condition to
identify the interactions between keratinocytes and fibroblasts. We also carried out several combined cultures
of normal or psoriatic keratinocytes and fibroblasts. In the culture fluid of'-combined cell-culture, the level
of interleukin-6 (IL-6) was significantly higher in the combination of psoriatic keratinocytes and fibroblasts
compared with that using normal keratinocytes. Psoriatic keratinocytes significantly secreted larger amounts
of transforming growth factor-α (TGF-α) compared with normal keratinocytes when they were solely cultured.
However, TGF-α secretion of normal keratinocytes was suppressed by co-culture with psoriatic fibroblasts, by
which psoriatic keratinocytes were not influenced. These results indicate that the altered interaction between
keratinocytes and fibroblasts exists in the psoriatic lesion, and it might occur in abnormal keratinocyte proliferation
The prevalence of Helicobacter pylori (H. pylori) infection and the effect of its eradication in 22 patients with idiopathic thrombocytopenic purpura (ITP) were studied. H. pylori infection was detected in 18 patients, and its eradication was obtained in 15 of 17 infected patients (88.2%). H. pylori eradication led 8 patients to a complete response (platelets > 100x10⁹/L), and 1 to a partial response (platelets 50-99x10⁹/L) (effective rate 60%). The patients with low level of PAIgG before eradication tend to achieve complete response compared with the other cases. Toxic effects of Grade 2 of skin rash appeared in 3 patients and Grade 1 of diarrhea in 2 patients, and all patients could continue the medication without severe toxic effects. H. pylori eradication may be considered as the first line therapy for ITP.
It is known that glaucomatous optic neuropathy is caused by selective cell death of retinal ganglion cells (RGC) by elevated intraocular pressure (IOP) and other causes. In the present study, we investigated RGC death in experimental glaucoma model rats (22 rat eyes) produced by cautery of three episcleral/limbal veins. As controls, 10 rat eyes with normal IOP were used. After cautery of episcleral veins, the average of IOP was significantly higher as compared with controls during 6 months after the operation. The number of retrograde labeled RGCs was significantly decreased in high IOP eyes as compared with normal IOP eyes. Therefore the episcleral/limbal veins occlusion model gave a consistence that long-term elevation of IOP leads to loss of RGCs and is useful as an experimental glaucoma model.
Background: The objective of this study was to investigate the role of immediate cerebral angiography
and local thrombolysis for stroke after cardiovascular surgery. Patients and methods: Aggressive approach including early cerebral angiography and intraarterial administration
of fibrinolytic agent for stroke after cardiovascular surgery was employed. Seven patients who had stroke after
surgery among 1244 patients who underwent cardiovascular surgery were enrolled this investigation. Results: In-hospital cerebral thrombosis or thromboembolism occurred 3 to 9 days (average: 6.3 ± 2.7 days)
after surgery. Immediate cerebral angiography revealed cerebral embolism in 4 and cerebral thrombosis in 3
with occlusion or stenosis of intracranial arteries. Local, intraarterial administration of a thrombolytic agent was
carried out in 5 patients with cerebral thromboembolism (n=4) or thrombosis (n=1), and complete recanalization
of the occluded artery was obtained in 3 patients (60%) and partial recanalization in 2 patients (40%). Additional
angioplasty for basilar artery stenosis was performed in one patient. No patients exhibited bleeding complication.
All patients survived with moderate or full functional recovery. Conclusions: Immediate cerebral angiography with/without local thrombolysis may improve functional outcome
and survival in patients with postcardiotomy cerebral thromboembolism.