Hirosaki Medical Journal
Online ISSN : 2434-4656
Print ISSN : 0439-1721
Volume 59 , Issue 1
Showing 1-6 articles out of 6 articles from the selected issue
Original Article
  • Dong-Liang Hu, Ai-Dong Qian, Xiao-Feng San, Kouji Narita, Hiroshi Sash ...
    2007 Volume 59 Issue 1 Pages 1-6
    Published: 2007
    Released: September 07, 2021
    JOURNAL FREE ACCESS
        A total of 179 samples of meat and 35 samples of egg from Japan and China were examined for the pollution of Escherichia coli, Salmonella enterica serovar Enteritidis and Staphylococcus aureus using the identical assay methods. The results showed that the pollution rates of E. coli, S. Enteritidis and S. aureus were 46.4 % , 4.4 % and 36.2 % in Japan and were 37.9 % , 5.5 % and 44.1 % in China, respectively. The pollution rate of E. coli in Japan (46.4 %) was higher than that in China (37.9 %). In contrast, the pollution rate of S. aureus in China (44.1 %) was higher than that in Japan (36.2 %). For the bacterial numbers in the contaminant meats, 4 of 69 samples in Japan and 4 of 145 samples in China were over 10⁴ colony-forming units (CFU)/g for E. coli, and 2 of 69 samples in Japan and 7 of 145 samples in China were over 10⁴ CFU/g for S. aureus. These results suggest that more thorough temperature control and hygiene management are necessary in the processing, safekeeping, circulation and sales of meats.
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  • Ken Harada, Katsuto Tamai, Hajime Nakano, Chiaki Sasaki, Katsumi Hanad ...
    2007 Volume 59 Issue 1 Pages 7-14
    Published: 2007
    Released: September 07, 2021
    JOURNAL FREE ACCESS
        230-kD bullous pemphigoid antigen (BPAG1) is known as an autoantigen in bullous pemphigoid and is expressed exclusively in proliferating basal keratinocytes. TGF-β is a growth factor that has pleiotropic effects on a wide range of target cells and induces differentiation of basal keratinocytes. Therefore, TGF-β is postulated to inhibit BPAG1 expression. However, previous report conversely demonstrated an increase of BPAG1 expression by TGF-β. In this study, to understand regulatory role of TGF-β on BPAG1 functions, we examined the effect of TGF-β on BPAG1 gene expression using cultured keratinocytes. This study showed that BPAG1 mRNA expression was inhibited by TGF-β1 in concentration higher than 1.0 ng/ml. Furthermore, incubation of the cells with TGF-β1 in the presence of cycloheximide demonstrated that newly synthesized protein was required for BPAG1 regulation. To understand the detailed mechanisms of BPAG1 modulation by TGF-β, we preformed transient transfection assay with a BPAG1 promoter-CAT construct to know the detailed mechanisms of BPAG1 modulation by TGF-β. The results revealed that calcium and IFN-γ inhibited BPAG1 expression at transcriptional level, but TGF-β1 is not responsible for that transcriptional inhibition, suggesting that TGF-β may have differential molecular mechanism for down-regulation of BPAG1 gene expression from the events induced by IFN-γ.
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  • Takaya Murai, Katsuto Tamai, Hajime Nakano, Katsumi Hanada, Isao Hashi ...
    2007 Volume 59 Issue 1 Pages 15-22
    Published: 2007
    Released: September 07, 2021
    JOURNAL FREE ACCESS
        Dystrophic epidermolysis bullosa (DEB) is caused by mutations in the COL7A1 gene encoding type VII collagen. DEB is clinically characterized by mucocutaneous blistering in response to minor trauma, followed by scarring and nail dystrophy. DEB is inherited in either an autosomal dominant (DDEB) or recessive (RDEB) fashion. DDEB basically results from a glycine substitution mutation within the collagenous domain on one COL7A1 allele, while a combination of mutations such as premature termination codon, missense, splice-site mutations on both alleles causes RDEB. In this study, we examined a Japanese EB boy with generalized blistering and scar formation, and made a diagnosis of the Hallopeau-Siemens type RDEB (HS-RDEB), the most severe form of RDEB. Mutational analysis of the COL7A1 gene revealed a novel missense mutation A80P and a novel nonsense mutation Q1211X. In general, HS-RDEB is caused by combination of premature termination codon mutations, but 3 HS-RDEB cases have been reported to have combination of premature termination codon and missense mutations one of which was S48P. This study suggests that even missense mutation, which leads to substitution for proline in amino terminal end of type VII collagen, can cause HS-RDEB.
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  • Ikuyo Ohguro, Hiroshi Ohguro, Mitsuru Nakazawa
    2007 Volume 59 Issue 1 Pages 23-32
    Published: 2007
    Released: September 07, 2021
    JOURNAL FREE ACCESS
    Purpose: To investigate the effects of anthocyanins in black currant on retinal blood flow circulation of patients with normal tension glaucoma (NTG).
    Methods: Thirty consecutive patients with NTG were included in this study. They were orally administrated anthocyanins extracted from black currant in tablet form once a day for a 6-month period. Systemic blood pressures. intraocular pressures (IOPs), concentrations of the plasma endothelin-1 (ET-1), blood flows at the neuroretinal rim of the optic nerve head and peripapillary retina, and visual field defects were measured before and just after the administration period.
    Results: Our study demonstrated that oral administration of anthocyanins tablets significantly increased the blood flows at both neuroretinal rim of the optic nerve head and peripapillary retina (p < 0.05), with no significant changes in mean blood pressures or IOPs. Furthermore, none of the subjects showed progression of their visual field defects. We also demonstrated that the oral administration of anthocyanins tablets significantly increased, and thus normalized the concentrations of plasma ET-1 (p < 0.05).
    Conclusion: These results suggest that anthocyanins orally administrated might be a safe and valuable choice for neuroprotective treatment of patients with NTG.
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  • Mamiko Mayama, Katsuto Tamai, Kazuyoshi Fukai, Toshifumi Nakagawa, Ken ...
    2007 Volume 59 Issue 1 Pages 33-40
    Published: 2007
    Released: September 07, 2021
    JOURNAL FREE ACCESS
        Dystrophic epidermolysis bullosa (DEB) is a heritable mechanobullous skin disease derived from mutations in the type VII collagen gene (COL7A1). DEB cases are divided into dominant dystrophic EB (DDEB) and recessive dystrophic EB (RDEB). Most of the DDEB cases are induced by glycine substitution (GS) mutation because of its dominant negative effect, although there are silent GS which are not pathogenic without combination of other mutation in COL7A1. To know the relations between clinical features and COL7A1 mutations, we examined COL7A1 gene mutation in two Japanese families with DEB, one is dominantly inherited and another is sporadic.
    We identified three mutations; 8068del17ins2 in case 1, G2395D/152delG in case 2. Case 1 is DDEB, which does not result from GS but from insertion/deletion mutation. In case 2, GS does not result in DDEB but causes recessive DEB (RDEB) with the combination of premature termination codon (PTC) in non-collagenous amino-terminal region (NC-1). This study demonstrates novel COL7A1 mutations for DEB and furthers our understanding of genotype-phenotype correlation displayed in DEB patients.
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  • Kazuaki Yoshikawa, Kazuyuki Mori, Seiji Kudoh, XingXing Liu, Takahiro ...
    2007 Volume 59 Issue 1 Pages 41-48
    Published: 2007
    Released: September 07, 2021
    JOURNAL FREE ACCESS
    AIM: Intravesical Bacillus Calmette-Guérin (BCG) therapy is an effective treatment for superficial bladder cancer. However, its frequent and severe side-effects were major obstacles for clinical setting. In this study, BCG was fractionized with simple methods to find an active component.
    METHODS: Sonicated or autoclaved Tokyo 172 BCG strain was fractionated to supernatant and precipitate. These fractions were co-cultured with 5637 human bladder cancer cell line (5637 cell). The growth inhibitory effect on 5637 cell was analyzed by dye exclusion test. ³H-thymidine incorporation, cytologic examination with Giemsa staining, and cell cycle analysis using flowcytometry.
    RESULTS: Live BCG and supernatant fractions obtained by sonication or autoclave suppressed the growth of 5637 cell. The dark-stained spots suggesting early phase apoptosis were found in nuclei of 5637 cells by co-cultured with live BCG or supernatant fractions. In these cells, the ratios of apoptotic cells were increased compared with non­ treated cells.
    CONCLUSION: These results suggest that BCG has a direct anti-tumor effect to induce apoptosis on 5637 cell. Supernatant fractions obtained by sonication or autoclave maintained the direct anti-tumor effects as well as live BCG. Further purification of these fractions may provide a new BCG derivatives which has higher efficacy with reduced toxicity.
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