Hirosaki Medical Journal
Online ISSN : 2434-4656
Print ISSN : 0439-1721
Volume 63 , Issue 2-4
Showing 1-7 articles out of 7 articles from the selected issue
Original Article
  • Haruyuki Ito, Ippei Takahashi, Masashi Matsuzaka, Sangun Lee, Kazuyosh ...
    2012 Volume 63 Issue 2-4 Pages 85-95
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
    Objective: We investigated the relationship between trace element concentrations and neutrophil basal reactive oxygen species (ROS) production capability among the Japanese general population.
    Subjects and methods: Subjects of the current study were 606 participants (225 males and 381 females) who had participated in the Iwaki Health Promotion Project 2005. Body mass index, biochemical blood examination, neutrophil basal ROS production and serum concentrations of trace element (Copper (Cu), Zinc (Zn), Iron (Fe), Selenium (Se) ) were measured. Each trace element was divided into 4 quartiles according to the concentration, and the neutrophil basal ROS production of each group was compared and analyzed. Moreover, the relationship between the concentration and neutrophil basal ROS production capability was assessed using a multiple linear regression analysis.
    Results: For both males and females, a tendency to low neutrophil ROS production associated with higher Se serum concentration was observed. On the other hand, no significant correlation was observed between the Cu, Zn and Fe concentrations and neutrophil basal ROS production capability.
    Conclusion: In conclusion, serum Se relieves oxidative stress through inhibiting neutrophil ROS production within a physiological range. On the other hand, our results suggested that neutrophil ROS production was unaffected by Cu, Zn and Fe concentrations within the normal physiological range.
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  • Fujimi Kudo, Naoki Nishiguchi, Kyoko Ito, Manabu Nakano, Koichi Ito
    2012 Volume 63 Issue 2-4 Pages 96-104
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
        The P2Y14 receptor is activated by UDP-glucose (UDPG), which is a well-known glycosyl donor that participates in the biosynthesis of carbohydrates, and is widely expressed in immune cells. During inflammation and mechanical stress, damaged cells release nucleotides, including ATP and UDPG, as danger signals that act as P2Y receptor agonists. These nucleotide-induced signals participate in the regulation of immune responses. In this study, to investigate P2Y14 expression further, we performed flow cytometric analysis using an anti-P2Y14 monoclonal antibody. The results indicated that P2Y14 is expressed in murine immune cells, including T cells, B cells, monocytes, granulocytes, and CD11bhigh macrophages. Interestingly, the expression levels of P2Y14 differed between immature and mature monocytes, and in CD11bhigh macrophages, P2Y14 was gradually downregulated as peritonitis was terminated. The expression of CD11b is reduced by the efferocytosis of apoptotic neutrophils during peritonitis, and the induced CD11blow macrophages, which emerge in the resolution of peritonitis, play an important role in the termination of inflammation. Consistent with this observation, we revealed that administration of UDPG to mice with induced peritonitis increased the number of CD11blow macrophages. As P2Y14 is expressed in CD11bhigh macrophages but not in CD11blow macrophages, UDPG may participate in the conversion to the CD11blow phenotype. These data suggest a novel regulatory pathway of the inflammatory response via P2Y14 expressed on macrophages.
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  • Shizuka Kurauchi, Ippei Takahashi, Masashi Matsuzaka, Kaori Iwane, Kaz ...
    2012 Volume 63 Issue 2-4 Pages 105-115
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
        Helicobacter pylori infected gastric mucosa causes chronic inflammation which induces atrophic gastritis (AG), and causes gastric cancer. It is suggested that selenium (Se) has preventive effects on gastric cancer incidence. However, the association between H. pylori infection/AG and the serum Se level has not yet been clarified. In this research, we investigated this association in a general population.
        The subjects were 728 people (252 men and 476 women) who participated in the health check-up during the Iwaki Health Promotion Project. The levels of IgG antibody for H. pylori in serum, H. pylori antigen in stools, serum pepsinogen I, serum pepsinogen II and serum Se concentration were measured.
        Serum Se level was decreased by both H. pylori infection and AG, and it negatively correlated with serum pepsinogen I level. Thus, this study suggests that AG with gastric cancer decreases serum pepsinogen I secretion, and leads to decreased absorption of Se.
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  • Akihito Kon, Yusuke Tando, Miyuki Yanagimachi, Hikaru Tanaka, Yuki Mat ...
    2012 Volume 63 Issue 2-4 Pages 116-126
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
        The purpose of this study was to analyze glycemic variability and insulin resistance of different types of diabetes, emphasizing mainly pancreatic diabetes, using Continuous Glucose Monitoring System (CGMS). The study involved a total of 59 patients receiving care at our department: 11 with pancreatic diabetes, 37 with type 2 diabetes, and 11 with type 1 diabetes. The CGMS data and several markers of insulin resistance were compared among the 3 groups and correlations were analyzed statistically. Standard deviation (SDCGMS) measured with CGMS and high molecular weight (HMW) adiponectin (Ad) as parameters of insulin resistance were identified as factors strongly affecting glycemic variability and insulin resistance. The magnitude of glycemic variability in patients with pancreatic diabetes was as large as that in patients with type 1 diabetes. Insulin resistance in patients with pancreatic diabetes was comparable to that in patients with type 1 diabetes. Our results suggest that CGMS data allow evaluation of both glycemic variability and insulin resistance in patients with pancreatic diabetes. HMW-Ad also appears to be a useful parameter of insulin resistance in patients with pancreatic diabetes.
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  • Jun Kondo, Fuyuki Sato, Yunyan Wu, Hiroko Seino, Satoko Morohashi, Hir ...
    2012 Volume 63 Issue 2-4 Pages 127-135
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
        Claudin is one of tight junction proteins which connect with the actin cytoskeleton and participate in the intracellular signaling. However, the significance of claudin in pancreatic cancer is understood not yet extensively. We examined the relationship between claudin-1 and invasion in PANC-1 and MIA PaCa-2 human pancreatic cancer cells, and investigated the functions of claudin-1 in invasive growth of pancreatic cancer cells. Claudin-1 knockdown by siRNA (claudin siRNA) affected the subcellular localization in the pancreatic cancer cells, and claudin-1 siRNA increased numbers of invasive pancreatic cancer PANC-1 and MIA PaCa-2 cells. Claudin-1 siRNA did not significantly affect expression levels of β-catenin, E-cadherin, α-smooth muscle actin, Bcl-2, and Bax in PANC-1 and MIA PaCa-2 cells. In addition, claudin-1 siRNA showed no significant change in the cell proliferation. We concluded that claudin-1 is significantly associated with invasive growth of human pancreatic cancer cells.
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  • Shunji Narumi, Reiichi Murakami, Shingo Hatakeyama, Tadashi Fujita, Ta ...
    2012 Volume 63 Issue 2-4 Pages 136-142
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
    Introduction: Kidney transplantation has been widely accepted as a definitive therapy for patients with renal failure. The Hirosaki Kidney Transplant Unit was organized in June 2006 in conjunction with the Departments of Urology, Cardiology, Respiratory Medicine and Nephrology, and Gastrointestinal Surgery, from the Hirosaki University School of Medicine. Herein, we introduce our current results and discuss our future strategies.
    Patients and Methods: From June 2006 to December 2011, 36 kidney transplants were performed with 31 living donors and 5 deceased donors. Immunosuppression therapy included an inductor treatment of anti-CD25 antibody and triple therapy with calcineurin inhibitor, mycophenolate mofetil, and steroids.
    Results:Recipients included 25 males and 11 females. The patients' average age was 41.8 years. Nine living–pairs were ABO incompatible. Deceased donors were performed at Oyokyo Hospital. Median follow-up period was 27.6 months. Acute cellular rejection occurred in 8.3% of patients. Positive antigenemia for cytomegalovirus happened in 16.7% of patients, but none developed invasive diseases. All recipients are currently surviving. Graft survival rates at 1, 3, and 5 years are 100%, 94.7%, and 94.7%, respectively.
    Conclusion: Successful kidney transplantations have been performed by a multidisciplinary unit at Hirosaki University. Our next step is a promotion to increase organ donation.
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  • Yukiko Abe, Dong-Liang Hu, Katsuhiko Omoe, Ikuo Fukuda, Akio Nakane
    2012 Volume 63 Issue 2-4 Pages 143-153
    Published: 2012
    Released: July 13, 2021
    JOURNAL FREE ACCESS
        Coagulase-negative staphylococci (CNS) are increasingly recognized to cause clinically significant infections. To investigate relationship of antibiotic resistance profiles, staphylococcal cassette chromosome mec (SCCmec) and superantigenic toxin genes in CNS, a total of 79 clinical CNS isolates from patients with surgical operations were comprehensively determined for minimum inhibitory concentration (MIC) of antibiotics, and searched for SCCmec and staphylococcal superantigenic toxin genes by novel multiplex PCR. Among 79 CNS isolates, at least one SCCmec gene was detected in 55 isolates (69.6%). The most of SCCmec type was type III (36.7%), and other detected types were type IVa (20.3%), type II (19.0%), type V (16.5%), type I (15.2%), and type IVb (3.8%). The isolates with type I or IVa gene showed highly resistance to clindamycin and gentamicin, the isolates with type II showed more resistance to teicoplanin, the isolates with type III or type V showed more resistance to clindamycin. se and tst-1 genes were detected in none of the CNS isolates tested. These results suggested that there is high prevalence of SCCmec in clinical CNS isolates, and the SCCmec types are related to the resistances against specific antibiotics, but SCCmec do not related to superantigenic toxin genes in the CNS.
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