This study examined discrimination learning in Sprague Dawley rats, particularly focusing on their choice behavior by observing a demonstrator ICR mouse, and the types of food sites involved. The subject rats had previously learned to differentiate between two types of food sites, replenished and depleted, through an earlier study conducted by the authors. In the first trial of the previous study, rats had access to four food sites in an experimental field. In subsequent trials, two specific sites were always replenished with food (replenished sites), while the other two were not (depleted sites). In the present study, a demonstrator mouse selected the food sites first, acting as a discriminative cue for the subject rats. In the high congruent group, rats were rewarded for choosing the site visited by the demonstrator mouse at replenished sites, and for choosing sites not visited by the demonstrator at depleted ones. Conversely, in the low congruent group, rats were rewarded for selecting sites visited by the demonstrator at depleted sites, and for selecting sites not visited by the demonstrator at replenished ones. Both groups showed better performance at the depleted sites than at the replenished sites. Although the high congruent group demonstrated marginally better performance than the low congruent group, the difference in performance did not reach statistical significance. This study is the first to demonstrate that rats can learn a discrimination task in which the behavior of mice from different species serves as discriminative cues for food rewards. However, further research with a larger sample size is necessary to more robustly demonstrate the rats’ ability to use the behavior of other animals as discriminative cues indicating the presence or absence of food.
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