Intersubspecific hybridizations among common toads were made, and the morphological characters of the hybrids thus obtained were examined. Females of
Bufo bufo japonicus from Momoyama, Kyoto were crossed experimentally with males of
B. b. miyakonis from Miyako Is., Ryukyu Archipelago and a male of
B. b. spinosus from Banyuls, Southern France. Early development of hybrid embryos proceeded in the same normal way as the control embryos, although the percentages of normally cleft eggs were slightly lower in the former than in the latter. Most of the normally cleft hybrid eggs became normal swimming tadpoles and attained the completion of metamorphosis. The
japonicus _??_ ×
miyakonis _??_ hybrid tadpoles had longer tails than the control, while the hybrid
japonicus _??_ ×
spinosus _??_ tadpoles had the smallest tails. Immediately after metamorphosis, the hybrid young toads had shorter hindlegs than the controls and the older young hybrids of two to four months after metamorphosis differed markedly from the controls in this respect. Some of the male hybrids of
japonicus _??_ ×
miyakonis _??_ attained sexual maturity in the fall. Histological examinations of the young toads revealed that both male and female gonads of
japonicus _??_ ×
miyakonis _??_ were developing normally, whereas no normal spermatogonia were developing in the testes and the ovarian structures were completely destroyed in the
japonicus _??_ ×
spinosus _??_ hybrids. These results seem to indicate that the genetical isolation is somewhat advanced between Japanese
japonicus and French
spinosus, and that they could be treated as two distinct species.
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