This cross-sectional study examined the relationship between daily physical activity, short-chain fatty acids (SCFAs) concentration, and abundance of SCFA-producing bacteria in 30 healthy young adults (15 males and 15 females) aged 20-23 years. Participants provided fecal sam-ples for gut microbiota and SCFA analysis. Daily physical activity, including step count and brisk walking time at an intensity of ≥ 4 metabolic equivalents (METs), was assessed. Participants were categorized into two groups (more and less active) based on their daily brisk walking time at a 4METs intensity and step count. We compared SCFA concentration and abundance of SCFA-producing bacteria between the groups and explored the relationship between the abundance of SCFA-producing bacteria and daily physical activity levels. No significant differences in the ma-jor SCFAs (acetic acid and butyric acid) were observed between the groups. A lower abundance of Ruminococcaceae, Faecalibacterium, and Faecalibacterium prausnitzii were observed in the active group. Overall, the relative abundance of intestinal Eubacterium, contributing to butyrate concen-tration, was associated with daily brisk walking at a 5METs intensity. In conclusion, while daily brisk walking did not directly affect SCFA concentration, it may be associated with the relative ab-undance of intestinal Eubacterium in healthy young adults.
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