International Journal of Oral-Medical Sciences
Online ISSN : 2185-4254
Print ISSN : 1347-9733
ISSN-L : 1347-9733
Volume 13, Issue 2
Displaying 1-6 of 6 articles from this issue
Original Articles
  • Kyung Mi Kim, Zhitao Wang, Ayako Kato, Yorimasa Ogata
    2015 Volume 13 Issue 2 Pages 35-44
    Published: 2015
    Released on J-STAGE: March 24, 2015
    JOURNAL FREE ACCESS
    Thalidomide is an immunomodulatory drug and primarily prescribed as a sedative and hypnotic. Treatment with thalidomide inhibited osteoblast development. Bone sialoprotein (BSP) is a mineralized tissue-specific protein that appears to function in the initial mineralization of bone. BSPexpression is essentially restricted to differentiated cells of mineralized connective tissues and that the expression of BSPis developmentally regulated. To determine molecular mechanisms involved in the suppression of bone formation we have analyzed the effects of thalidomide on BSPgene expression. Thalidomide (10 μg/ml) decreased BSPmRNA levels at 12 h in ROS 17/2.8 osteoblast-like cells. Transient transfection analyses, using chimeric constructs of the rat BSPgene promoter linked to a luciferase reporter gene, revealed that thalidomide (10 μg/ml, 12 h) suppressed luciferase activities of pLUC3 (nts −116 to +60), pLUC4 (nts −425 to +60) and pLUC5 (nts −801 to +60) transfected into ROS17/2.8 cells. Further deletion analysis of the BSPpromoter showed that a region within nts −43 to −84 was targeted by thalidomide. Introduction of 2 bp mutations in the inverted CCAAT box (ATTGG; nts −50 and −46), a putative cAMPresponse element (CRE; nts −75 to −68), a fibroblast growth factor 2 (FGF2) response element (FRE; nts −92 to −85) and a pituitary-specific transcription factor-1 (Pit-1) binding site (Pit-1; nts −111 to −105) showed that the thalidomide effects were mediated by the CRE and inverted CCAAT box. Gel mobility shift analyses showed that thalidomide increased nuclear protein binding to CRE. Whereas,inverted CCAAT box binding proteins did not change after stimulation by thalidomide. Antibodies against CRE-binding protein 1 (CREB1), phospho-CREB1, c-Fos, c-Jun, JunD and CREB2 disrupted CRE-protein complex formation. These studies, therefore,demonstrated that thalidomide suppresses BSPgene transcription mediated by CRE and inverted CCAAT box in the rat BSPgene promoter.
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  • Toshikazu Uchiyama, Hiroyuki Tanaka, Kazumune Arikawa, Chieko Taguchi, ...
    2015 Volume 13 Issue 2 Pages 45-52
    Published: 2015
    Released on J-STAGE: March 24, 2015
    JOURNAL FREE ACCESS
    In 2011, physical and operational changes were made to improve our institutionʼs dental human dock clinic. The physical change was consisted of aesthetic improvements to the comprehensive checkups being performed in individual rooms. The operational changes were improved diagnostic accuracy and increased the number of tests available to patients as part of the oral dock checkup, namely, adopting a test of caries risk (testing for bacteria that cause tooth decay), an IgG antibody titer test for periodontal pathogens using blood collected from the fingertip, a bite test and a halitosis test, as well as adding more staff members to the examination team. After these improvements were implemented, we conducted a survey to assess patient satisfaction at the clinic. The survey asked patients to provide their overall impression of the clinic and to rate facilities and equipment, dental care providers, clinical tests, evaluation reports and other aspects. A 10-point scale was used to rate overall impression and a 5-point scale was used to rate all other items. Survey forms were distributed to 74 patients who received the checkup and consented to participate, of which 46 responded (response rate: 62.2%). Patients were generally very satisfied with dental care providers and clinical tests as reflected by an average overall impression rating of 7.9. However, the average rating for facilities and equipment was low at 3.76. The other aspects category received the lowest rating, with cost being rated at 2.43.We plan to improve the clinicʼs operations by focusing on low scoring aspects of these findings.
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  • Ichirou Honda, Ichiro Ogura
    2015 Volume 13 Issue 2 Pages 53-58
    Published: 2015
    Released on J-STAGE: March 24, 2015
    JOURNAL FREE ACCESS
    The purpose of this study was to investigate the characteristic multidetector CT (MDCT) findings, especially the prevalence of location, of mandibular fractures resulting from falls and violence. A prospective study was performed in 217 patients with mandibular fractures resulting from falls or violence underwent 64-MDCT. Mandibular fractures were classified into four types: median, paramedian, angle and condyle. Statistical analysis of the relationship between cause and mandibular fracture location was performed using χ2 test and logistic multivariate regression analysis. The results showed that the percentages of cases resulting from falls and violence were 55.8% and 44.2% of paramedian type (p=0.001), 46.4% and 53.6% of angle type (p=0.000) and 87.8% and 12.2% of condyle type (p=0.000), respectively. This study indicated a significant relationship between cause and condyle type (odds ratio (OR)=3.303, p=0.009), paramedian type (OR=0.339, p=0.017) and angle type (OR=0.313, p=0.019), respectively. In conclusion, the results of the presented study suggest the characteristic MDCT findings, especially the prevalence of location, of mandibular fractures resulting from falls and violence.
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  • Mami Odaira-Yamazaki, Masaru Yamaguchi, Jun Kikuta, Mami Shimizu, Tomo ...
    2015 Volume 13 Issue 2 Pages 59-65
    Published: 2015
    Released on J-STAGE: March 24, 2015
    JOURNAL FREE ACCESS
     In this study, we first investigated the expression of Jagged1 at compression sites during experimental tooth movement in rats in vivo. We then assessed the effects of compression force on Jagged1 production from human periodontal ligament (hPDL) cells.
     Twelve 6-week old male Wistar rats were subjected to an orthodontic force of 10 g in order to induce mesial tipping movement of the upper first molars for 7 days. Expression levels of tartrate-resistant acid phosphatase and Jagged1 proteins in the alveolar bone were determined by immunohistochemical analysis. Furthermore, the effects of compression force on Jagged1 production were investigated using hPDL cells in vitro.
     Under conditions of experimental tooth movement in vivo, resorption lacunae with multinucleated cells were observed in the 10 g group. In addition, immunoreactivity for Jagged1 was detected on day 7 in PDL tissue subjected to orthodontic force. In the in vitro study, compression force increased the production of Jagged1 from the hPDL cells in a time-dependent manner.
     These results suggest that Jagged1 stimulates alveolar bone resorption during orthodontic tooth movement.
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  •  
    Hitomi Kurosawa, Shinichiro Aoki, Tomoyasu Ebihara
    2015 Volume 13 Issue 2 Pages 66-74
    Published: 2015
    Released on J-STAGE: March 24, 2015
    JOURNAL FREE ACCESS
     This study investigated brain cognition using P300 for fundamental examination of information-processing modes generated in tooth, script, and hand discrimination by dental students. Subjects were 19 second-year dental students. The task was to differentiate a tooth, script character, or hand in each of a rotated series of line drawings. Images were presented at random using the oddball paradigm with a target-to-non-target ratio of 2: 8,with the subject instructed to press a button only on observation of the target stimulus. Correct answer rate, reaction time, P300 amplitude, and P300 latency were analyzed using electroencephalography (EEG). Results of EEG were divided into waveform components,feature extraction of the waveform was performed, and the relevance of presented orientation was examined.
     Reaction time was longest for the 180° orientation with all tasks. No differences in P300 latency were seen by presented orientation for any task, but P300 amplitude was smallest at 180° for all tasks. Script characters and hands at 0° were easily discriminated, but tooth did not show any orientation difference.
     These findings suggest that discrimination in script and hand tasks was performed by top-down processing and bottom-up processing efficiently, and that discrimination in tooth task was intentionally performed in a bottom-up manner.
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  • Pooja Aggarwal, Susmita Saxena, Vandana Reddy, Preeti Sharma, Vikas Ag ...
    2015 Volume 13 Issue 2 Pages 75-80
    Published: 2015
    Released on J-STAGE: March 24, 2015
    JOURNAL FREE ACCESS
     AIM OF STUDY: Salivary gland tumors are uncommon, and the epidemiological data of these tumors in various parts of the world can be helpful for a better understanding of their biology and clinical characteristics. Thus, this study was an epidemiological survey of salivary gland tumors in the western Uttar Pradesh population.
     MATERIALS AND METHODS: This retrospective study was a clinical review ofour experience with different subtypes of salivary gland tumors, diagnosed from May 2004 to May 2010. The histological diagnoses were evaluated according to the 2005 WHO classification. These data were analyzed for the distribution of benign and malignant salivary tumors in minor salivary glands, male to female ratio, age range, and site of occurrence.
     RESULTS: A total of1805 specimens were received; ofthese, 4% were diagnosed as salivary gland tumors, with a male to female ratio of1:1.4. The mean age ofthe patients was 51.4±18.1 years. Ofthe tumors, 59.5% were benign and 40.5% were malignant neoplasms, with the most common tumors being pleomorphic adenoma and mucoepidermoid carcinoma.
     CONCLUSION: The present study provides demographic data on a series ofsalivary gland tumors in the western Uttar Pradesh population. This study confirmed that some tumors have a predilection for certain sites, and that the risk of malignant disease is also greater at specific sites within the oral cavity.
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