International Journal of Oral-Medical Sciences
Online ISSN : 2185-4254
Print ISSN : 1347-9733
ISSN-L : 1347-9733
Volume 5, Issue 1
Displaying 1-9 of 9 articles from this issue
Original Articles
  • Arlétte Suzy Setiawan, Lelani Reniarti, Roosje Rosita Oewen
    2006 Volume 5 Issue 1 Pages 1-5
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    Oral mucositis is one of a common, debilitating complication of cancer chemotherapy. Mucositis causes severe pain and distress, and may limit the tolerability of chemotherapy. The aim of this study was to compare the efficacy of chlorhexidine gluconate and povidone iodine mouthwashes on oral mucositis in children receiving chemotherapy for acute lymphoblastic leukemia (ALL). A total of 18 children age 2 to 10 years were participating in a clinical trial with pre-post test design and single blinded system. Subjects were divided into three groups, using chlorhexidine gluconate, povidone iodine, and alkaline saline as a control group, respectively. Clinical measurements to assess mucositis and pain were performed using General Mucositis Scale from WHO and Wong-Baker Faces Rating Scale, respectively. Children in all groups received daily oral hygiene instructions, and were examined daily until the mucositis heals. Data were analyzed by ANOVA and Newman-Keuls methods with significance value p=0.05. The results showed that mucositis and its related pain were disappeared after 5 to 7 days, 8 to 14 days, and 13 to 14 days using chlorhexidine gluconate, povidone iodine, and alkaline saline (control), respectively. It is concluded that chlorhexidine gluconate was more effective on the healing of oral mucositis than povidone iodine.
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  • Hideo Hosaka, Naomi Ogura, Toshirou Kondoh, Yoshimitsu Abiko
    2006 Volume 5 Issue 1 Pages 6-11
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    Elevated levels of IL-1β have been found in synovial fluid from patients with temporomandibular disorders (TMD) and are thought to be involved in pain and progression of TMD. Synovial cells in the temporomandibular joint have important roles in progression of synovitis ; however, their biological roles are poorly understood. In this study, we examined the effect of IL-1β on gene expression in synovial fibroblast cultures from 5 different patients. Gene expression was monitored by Affymetrix GeneChip analysis and real-time PCR. Gro-α protein level was measured by ELISA. Among the genes with altered expression, GeneChip analysis detected increased Gro-α mRNA in fibroblasts from all 5 synovial specimens. This increased Gro-α gene expression was confirmed by real-time PCR. Gro-α protein was preferentially increased by IL-1β in synovial fibroblasts. Gro-α has chemotactic effects on lymphocytes and monocytes, and it also regulates endothelial cell proliferation and stimulates angiogenesis. When these established effects are taken together with the results presented herein, increased gene expression and protein production of Gro-α in synovial fibroblasts in response to IL-1β appears to play an important role in recruitment of inflammatory cells into the synovium and in progression of synovitis.
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  • Toshitaka Yamazaki, Masanobu Matsuno
    2006 Volume 5 Issue 1 Pages 12-21
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    The dental anthropological survey of the Naxi tribe, one of the Chinese ethnic minorities, was carried out in Lijiang in Yunnan Province in 2002. The subjects were young adults whose dental impression models were collected, and the frequencies of 22 dental traits were recorded. The results were compared with those of previous studies including Chinese minorities, Dai and Hani, and with other Asian populations in terms of Turner's Mongoloid dental variation theory. A principal coordinate analysis based on Smith's Mean Measure of Divergence using the frequencies of 17 traits suggested that the Naxi tribe positioned close to Dai and Hani and belonged to the Sundadont dentition category, which typifies Southeast Asians, despite its Sinodont (Northeast Asia) location. Other anthropological and linguistic studies also suggest that the Naxi are closely related to Tibetans. With reference to our findings, and to presumed past and present distributions of Sinodonty and Sundadonty, we postulate that the Naxi in Yunnan Province have genetically inherited traits similar to the Sundadont peoples of Southeast Asian, but also exhibit environmentally induced traits similar to those of the Sinodont Han (who comprise the majority of the Chinese population) and other Sinodont tribes in their vicinity. Historical factors both genetic and environmental are, it is argued, reflected in their dental characteristics.
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  • Mika Sasaki, Kohtaro Fujihashi
    2006 Volume 5 Issue 1 Pages 22-30
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    The immunodeficiency and increased risk of infection seen in elderly patients may be attributable to an alteration in the immune system. Since dendritic cells (DCs) play a key role for the induction of antigen (Ag)-specific immune responses, we hypothesize that upregulation of aged DCs function by flt3 ligand and CpG ODN abrogate for the induction of Ag-specific adjuvant immune responses. Mononuclear cells were isolated from spleen of BALB/c mice (>24 months of age) and were stained with an array of fluorescence-conjugated mAbs in order to determine the frequencies of DCs by fluorescence activated cell sorter (FACS). The spleen from aged mice contained reduced frequencies CD8+ DCs and pDCs. Further, aged splenic DCs showed impaired APC function when compared with DCs from young adult mice. To compensate this age-associated alterations, flt3 ligand (FL) and CpG ODN were employed. When aged splenic DCs were cultured with recombinant (r) FL and CpG ODN, significant proliferative responses were induced, which is essentially identical to that of splenic DCs from young mice. Further, aged mice given nasal OVA plus FL plasmid (pFL) and CpG ODN showed high levels of OVA-specific IgG and IgA Abs in plasma which are comparable to those of young adult mice. Thus, the frequencies of CD8+ DCs and plasmacytoid DCs in spleen of aged mice were significantly increased after the immunization with pFL and CpG OND. More importantly, aged splenic DCs expressed increased frequencies of the costimulatory molecules. These results showed that a combination of pFL and CpG ODN treatment was an effective strategy to enhance impaired immunity that is seen in aged mice.
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  • Somsak Sirirungrojying, Tohru Hayakawa, Daijiro Meguro, Kazutaka Kasai
    2006 Volume 5 Issue 1 Pages 31-36
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    The aim of this study was to evaluate the tensile bond strength of 4-META/MMA-TBB resin to enamel using a self-etching primer with saliva contamination. The polished human molar surface was etched with phosphoric acid or Megabond self-etching primer with or without saliva contamination, then bonded with Superbond C&B resin cement. The tensile bond strengths were measured after immersion in water at 37 °C for 24 hours. Without saliva contamination, there was not significantly different between phosphoric acid etching and Megabond (P>0.05). When the surfaces were saliva contaminated, using phosphoric acid etching showed decreasing in bond strength significantly (P<0.05). Comparing to using Megabond, the bond strength decreased nonsignificantly (P>0.05). The SEM showed the difference of enamel surface after phosphoric acid etching and Megabond. In conclusion, comparing the tensile bond strength, Megabond is a better candidate for etching enamel than phosphoric acid when clinical used.
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  • Yasuhisa Tsujimoto, Tomohiro Katoh, Masahiro Kozuka, Takashi Tomita, M ...
    2006 Volume 5 Issue 1 Pages 37-42
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    Our dental university has allocated time for endodontic practice to 4th-grade students who have completed a 3rd-grade course in endodontics. In this term, students to practice access opening of extracted upper and lower human molars. All the teeth are donated by students themselves, and the effectiveness of this approach for teaching has been analyzed. Students found 3 root canal orifices in 82.1% of upper molars with 3 root canal orifices, and 3 root canal orifices in 87.5% of lower molars with 3 root canal orifices and 2 root canal orifices in 87.1% of lower molars with 2 root canal orifices. No appreciable difference in ease of access opening was observed between upper and lower molars. Perforation occurred about 20% more frequently in upper than in lower molars. Knowledge of tooth anatomy is essential for students studying endodontic practice. Future efforts should be directed at improving endodontic education to ensure that failure does not occur in practice.
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  • Masahide Hayakawa, Hiroyuki Okada
    2006 Volume 5 Issue 1 Pages 43-49
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    Keratocystic odontogenic tumor (KCOT), called formerly as odontogenic keratocyst (OKC), is newly classified in the odontogenic tumor by the revised WHO histological classification in 2005, because of its potential for aggressive behavior, high local recurrence rate and the involvement of PTHC gene mutation in etiology. We examined the cytokeratin expression and proliferative activity of KCOT/OKC and compared them to non-keratinized primordial cyst (PC), in order to better understand its nature. The epithelial layers of KCOT/OKC and PC revealed almost the same cytokeratin expression to CK 7, 13, 19, 20 and HMW, but showed the different expression to CK 17 (KCOT/OKC, + ; PC, -). The LIs for Ki-67 and topoisomerase IIα were higher in KCOT/OKC (23.1 ; 10.4) than in the PC (9.8 ; 3.6), and Student's t test demonstrated a significant difference (p<0.01) in both LIs between KCOT/OKC and PC. There was a strong correlation between the Ki-67 LI and the topoisomerase IIα LI (Pearson's correlation coefficient r=0.91), suggesting that their combined use is recommended to estimate the proliferative activity. CK 17 might be related to the proliferative lesions. It is considered that KCOT/OKC behaves biologically as a tumor and belongs a different entity to PC.
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  • Harunori Okamoto, Kiyoshi Arai, Kensuke Matsune, Saori Hirukawa, Sator ...
    2006 Volume 5 Issue 1 Pages 50-56
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    To evaluate the usefulness of new hydroxyapatite (HAP) as a pulp capping agent, comparisons with a calcium hydroxide agent (calvital®) and formocresol, which are generally used for pulpotomy, were performed. Serial changes in pH in these materials were investigated. Furthermore, after pulpotomy in rat molars using each material, the degree of the formation of dentine-like hard tissue and inflammatory changes in the pulp tissue were micro CT radiographically and patho-histologically examined. It has been reported that calvital® was a strong alkali (≥pH 12) causes a high level of pulp irritation, which were same as our result. The new sintered hydroxyapatite was almost neutral in pH, with a low level of pulp irritation. Furthermore, as a result of micro CT radiographical and histopathological evaluation, dentin-like hard tissue formation underneath the sectioned pulp surface was most marked in the calvital® group, and no dentin-like hard tissue formation underneath the sectioned pulp surface was noted in the HAP group. However, the HAP group developed no necrotic layers, and inflammation improved at an earlier stage.
    Our experiments suggested that HAP can be a good pulp capping agent, since pulp irritation was lower with HAP than with calvital® and formocresol.
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Case Report
  • Osamu Iwamoto, Chihiro Koga, Masatora Aoki, Akihiro Koba, Jingo Kusuka ...
    2006 Volume 5 Issue 1 Pages 57-59
    Published: 2006
    Released on J-STAGE: February 15, 2011
    JOURNAL FREE ACCESS
    A 74-year-old woman underwent hemilateral resection of the mobile tongue and right modified radical neck dissection after preoperative chemotherapy following a diagnosis of tongue cancer, T2N1M0. Fever and swelling of the left lower limb occurred 34 days after surgery. Computed tomography (CT) and ultrasonography (US) examinations suggested left femoral venous thrombosis. Immediately, anticoagulant fibrinogenolysis therapy for thrombosis was administered. Thereafter, fever and swelling of the left lower limb gradually subsided without complications such as pulmonary embolism. The presence of cancer, the patient's advanced age, major surgery for 5 hours, and preoperative chemotherapy were considered to the main factors for thrombus formation. To date, the patient's recovery has been good without recurrent thrombosis.
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