An attempt to estimate the radicals numbers formed from hydrogenperoxide by plasma discharging technique in the apparatus (STERRAD 100, Johnson & Johnson Med. Co.) has been done using ascorbic acid (A. A) and diphenyl picryl hydrazyl (DPPH) as radical indicator. Then, the radical numbers were reduced from the adsorbance difference at λ max. on the spectra (on A. A : ultraviolet, on DPPH : visible ray) between before and after sterilizing using the Loschmidt's number, respectively. In result, it was found that the radical numbers were similar to the molecule numbers of H_2O_2 fed into the sterilizer before sterilizing. Moreover, it was guessed that ・O radical mainly reacted on A. A and ・OH radical mainly reacted on DPPH by the spectroscopic and gas chromatographic techniques.
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