An attempt to estimate the radicals numbers formed from hydrogenperoxide by plasma discharging technique in the apparatus (STERRAD 100, Johnson & Johnson Med. Co.) has been done using ascorbic acid (A. A) and diphenyl picryl hydrazyl (DPPH) as radical indicator. Then, the radical numbers were reduced from the adsorbance difference at λ max. on the spectra (on A. A : ultraviolet, on DPPH : visible ray) between before and after sterilizing using the Loschmidt's number, respectively. In result, it was found that the radical numbers were similar to the molecule numbers of H_2O_2 fed into the sterilizer before sterilizing. Moreover, it was guessed that ・O radical mainly reacted on A. A and ・OH radical mainly reacted on DPPH by the spectroscopic and gas chromatographic techniques.
A newly developed nitric oxide (NO) microsensor was applied to NO measurements in isolated organs. The NO sensor was inserted into the vascular media of an isolated canine femoral artery, perfused with a Krebs-Henseleit solution. NO concentration in the media increased linearly with increasing perfusion flow rate (wall shear stress). NO production was attenuated by N^G-mono-methyl-L-arginine (L-NMMA). A dramatical change in NO concentration in the perfusate from an isolated beating rat heart was observed after 1-min flow suspension. NO concentration increased markedly during the first 30 sec of reperfusion and returned to the level before flow suspension after 3-4 min. NO release was enhanced by increasing perfusion pressure (70 to 100 cmH_2O), i.e., perfusion flow rate (wall shear stress). The newly developed NO microsensor showed satisfactory performance for measurement of NO in the vascular media and in the perfusate from the isolated heart.