A technique utilizing hydride generation-atomic absorption spectro-photometry was established for the fractional determination of triethyllead (Et3Pb
+), diethyllead (Et2Pb
2+), inorganic lead (Pb
2+) and total lead (total Pb) in the urine.
The extraction of the target lead compounds from the urine required the pre-treatment of urine samples.
Sample solutions were prepared by the use of 0.5 M DL-Malic acid for the generation of Et3Pb
+, by the use of 0.75 M H
20
2-0.004 M HCl0
4 for the generation of Et2Pb
2+, and by the use of 1.6 M DL-Malic acid-0.05 M K
2Cr
20
7 for the genera-tion of Pb
2+; and sodium borohydride was then added to the so treated sample solu-tions. The lead hydrides were once fixed in a U-trap cooled with liquid nitrogen beforehand, and the respective hydrides were then fractionated by volatization; and the fractions were atomized in a quartz cell heated to 1000°C for atomic absorption spectrophotometry.
The recovery rates by this technique from 5 replications proved to be 99.7% for Et3Pb
+, 97.3% for Et2Pb
2+, 91.4% for Pb
2+, and 95.9% for total Pb, with the coefficients of variation being 5.6% for Et3Pb
+, 7.5% for Et2Pb
2+, 2.1% for Pb
2+ and 2.9% for total Pb. The detection limits of this technique also proved to be 0.005 μgPb for Et3Pb
+ and Et2Pb
2+ and 0.1 μgPb for Pb
2+.
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