The workers, who had been exposed to dusts of stabilizers including lead stearate, lead orthosilicate, cadmium stearate and others in a vinyl chloride film manufacturing factory, were examind in 1963 and 1964. A high occurrence of proteinuria was observed without any change in the function of the respiratory organs. Their urinary excretion of cadmium was found to increase, though slight, but no increase in urinary lead ex-cretion. From the results, the proteinuria was supposed to be due to the absorption of cadmium stearate.
The sensitivity of mice poisoned subclinically with nitroglycol to administered adrenaline was investigated, especially that of mice that had been kept free from nitro-glycol for a certain period after daily nitroglycol injections. Mice received daily subcutaneous injection of 100 mg/kg of nitroglycol for 14 days. During and after nitroglycol treatment, adrenaline of 2 mg/kg was injected daily for 3 days. The mortality rate of mice to adrenaline increased temporarily at an early stage of the treatment, but decreased gradually with successive injection of nitroglycol and that of mice treated for 14 days were the same as non-treated mice. On the other hand, the mortality rate to administered adrenaline of mice that had been kept free from nitroglycol for 2 days after 14 days' treatment increased remarkably, but that of mice after 10 days' freedom from nitroglycol returned to the normal value again. These results indicate that abrupt cessation following long-term-administration of nitroglycol may produce the remarkable rise of sensitivity to administered adrenaline.
A previous paper dealt with the effect of environmental factors on experimental benzene poisoning. The present paper considers the role of host factors, in particular age, in modifying the toxicity of benzene. Experimental animals were male Donryu rats, and ICR-JCL mice of both sexes, which were given benzene in an olive oil mixture subcutaneously on the back for five days. Young rats, with the exception of baby rats, were found to have more susceptibility to benzene than mature ones. Mice, on the other hand, showed no significan t differ-ence between young and mature animals, though males were found more susceptible to benzene than females. The method of selection of animals for experiment is stated, and it is indicated that mature rats of over nine weeks of age may be suitable for experimental work in benzene poisoning, and that it may be unnecessary to take age into consideration in mice.
In alkali solution, diphenylcarbazide reacts with lead and produces a water soluble complex of deep red. The author intended to determine lead on the filter paper using this reaction. The fixed volume of lead solution was spotted on the filter paper and was developed with diphenylcarbazide. Then the area of developed spot increased logalithmically with the increasing concentration of lead solution. By this method, 150 γ of lead can be determined easily and quickly. When the lead solution was spotted on the narrow filter paper (2 mm or 4 mm in width), lead could be determined from the length of colored part. Limit of identification by this method is about 0.01γ lead, and limit of determination is about 0.1 γ lead.
A new spectrofluorometrical method is proposed for the determination of trace of 3, 4-benzpyrene in the benzene solution contaminated with 3, 4-benzfluoranthene or 1, 2-benzpyrene and 3, 4-benzfluoranthene. This method consists of the removing of oxygen dissolved in a test solution, measurement of fluorescence spectrum and the determination by narrow base line method proposed by Commins for the spectrophotometrical determina-tion of polynuclear hydrocarbons. By this method 3, 4-benzpyrene can be determined accurately even in the solution containing 3, 4-benzpyrene, 3, 4-benzfluoranthene and 1, 2-benzpyrene in a concentration ratio of 1 : 100 : 100.
The retention times on active carbon column were observed about thirteen organic vapors by gas chromatography. The organic compounds were n-alcohols of C1-C4, n-paraffines of C5-C6, acetone, methyl ethyl ketone, diethyl ketone, ethyl acetate, cyclohexane, benzene and carbon tetrachloride. The active carbon was a commercial activated wood charcoal produced for gas mask canisters. The retention times of organic vapors depended upon temperature of the column in accordance with Arrhenius' equation in the range of 60°C-250°C. In the homologous series of n-alcohols and ketones, the logarithmic retention times were in linear relation with their boiling points respectively at each temperature of 150°C, 200°C and 250°C. Retention time of each compound was extrapolated to 60°C by Arrhenius' relation to obtain their relative retention times to compare with that of carbon tetrachloride at that temperature, considering the national approval test of gas mask canisters where carbon tetrachloride was chosen as the test gas for masks.
Minerals and 3, 4-benzpyrene in particulate pollutants in winter air of Tokyo were studied by the methods developed by the authors. Minerals in air pollutants of Tokyo were quartz, feldspar, cristobalite, mica and kaolin minerals which were the mineral assemblage of Kanto loam constituting earth surface of Kanto plane. The highest con-centration of quartz in the air during this survey was 0. 034mg/m3, and mean concentra-tion of 3, 4-benzpyrene in Tokyo air was 15.1μg/1000m3. Obtained data were discussed in relation to "Tokyo-Yokohama asthma" and lung fibrosis.