In a factory a worker died shortly after he received an accidental pour of the n-butylpyrrolidine over his upper trunk. In this connection animal experiments were undertaken to obtain some information on toxic properties of this substance. Intraperitoneal, subcutaneous and oral LD50 values of n-butylpyrrolidine in mice were 37. 3, 56.8 and 51.1 mg/kg respectively. In this respect n-butylpyrrolidine should be regarded as a highly toxic substance. Outstanding feature of this acute poisoning in mice was immediate onset of clonic convulsion with a fatal outcome in several minutes. Once the attack was overcome, the mice showed rapid recovery. Application of n-butylpyrrolidine on sheared skin also revealed its lethal toxicity without any local injury. The LD50 in the skin test was about 1.0 ml/kg and rapid absorption from intact skin was suggested. Daily administration of n-butylpyrrolidine, even at the dose of 20 mg/kg, were tolerated in mice at least until 4 weeks without any evidence of abnormality.
The retention of mercury to the thyroid was investigated with the help of radioactive mercury. Rat or rabbit was injected with the solution of mercuric acetate, phenyl mercury acetate or methyl mercury acetate. The extent of retention of mercury in the thyroid on 7th and 14th days after injection varied with the sort of mercury compound administered. A considerable high concentration was, however, found in any mercury compound. The height of follicle cell in the thyroid of rat increased and its variance became larger on 14th days after injection in all three compounds. In not the colloid, but the cell of the rabbit's thyroid, the mercury of three mercury compounds had high concentrations.
Carbon tetrachloride was administered to intact and cervical cord-sectioned rats.Centrilobular hepatic cell necrosis and triglyceride accumulation were suppressed by the preceding cord-section. And the calcium content which increased markedly in the late stage of CCI4 poisoning also remained low in the operated animals. However, activity of glucose-6-phosphatase of liver homogenate and in vivo incorporation of 1-14C-glycine into liver protein and into plasma albumin fell strikingly in both the operated and intact rats. In accordance with the biochemical findings, mitochondrial disintegration seen in the late stage of poisoning in unoperated rats was almost completely lacking in cord-sectioned animals, while the alterations in ER system were extensive in the both groups. These results indicate that in cord-sectioned rats there is a discrepancy between the changes of mitochondria and ER in CCI4 poisoning.
An automated method for the determination of adrenaline and noradrenaline in the eluate from alumina is presented. In this method, citrate was added to the alumina eluate to prevent the interference of aluminum hydroxide produced at neutral pH for the determination. For the differential estimation of the two catecholamines, two filter sets for activation and emission in fluorometry were used. The optimum concentrations of reagents for the determination were investigated. Almost all catecholamines in the acetate buffer solution were recovered by the present method, and the recovery of catecholamines added to the alumina eluate of human urine was 90 to 95%. Correlationship between the data obtained by the method of Euler and Lishajko1) and those by the present method was as follows; coefficients of correlation were 0.941 and 0.978 and regression lines were y=0.86x+4.4 and y' = 1.05x'-2.4 for adrenaline and noradrenaline respectively (N=50, y y' for the automa-ted method and x, x' for the manual method).
In thin layer chromatography, band width of a sample applied to a plate is one ofthe most important factors affecting on the efficiency of separation of a sample. This paper describes a new method to narrow and to separate adequately a band of mixture of polynuclear hydrocarbons applied to a chromatoplate by chromatocharger. This method uses a chromatoplate composed of two adsorbent layers. One layer is used to narrow a band of sample applied to a plate and the other is used to separate the narrow band into several bands corresponding to each hydrocarbon. A successful separation of polynuclear hydrocarbons is obtainable by thin layer chromatography using 26% acetylated cellulose-aluminium oxide G plate, silicagel WOELM-cellulose plate and aluminium oxide G-cellulose plate. By this method several hydrocarbons in 10 ml of dilute solution can be easily separated into each hydrocarbons without any concentrationtreatment.
Method for the determination of retention of inhaled particles in the human res- piratory tract was studied. Aerosol concentration was determined by light scattering, respiratory air flow by pneumotachograph, and carbon dioxide content by infrared analyzer (Capnograph). These were recorded simultaneously and continuously during breathing aerosol. Detector or parts of these measuring apparatus were inserted into the mouthpiece of which inner space was only 9.5 ml. This extreme reduction of the dead space of mouthpiece enabled the measuring apparatus to work with quick response. By this method, the retention of lead fume in the respiratory tract was studied. Lead fumes with various sizes from 0. 05 μ to 1 μ were prepared by high frequency induction furnace. Total retention rate of lead fumes decreased with particle size, for instance, it was 63.2% for 1 μ particle but 42.5% for 0.05 μ at 10 respirations per minute. And the increase of respiratory rate resulted in the drop of retention. Reten- tion rates in the upper and lower respiratory tracts were also determined.
The apparatus of the monodisperse metal fume generator was designed and the productions of lead fumes were studied systematically. To get the stable heating on the evaporation of metal, the high frequency induction furnace was used. The generated fumes were carried with nitrogen gas which was dried and filtered. Then, the lead fumes, having variable mean diameter with the narrow size distribution, could be generated with changing flow rate of the carrier gas and temperature of the furnace.