Plasma level of 11-hydroxycorticosteroids and urinary excretion of catecholamines and 17-ketogenic steroids were measured on fresh workers newly graduated from school at the first and the third month of employment. The values of adrenaline excretion and plasma 11-hydroxycorticosteroids of the new workers at the first month were significantly higher than those of the senior members in the same working place and than their own values at the third month. As adrenal cortical and medullary hormone levels have been reported by many au- thors to be elevated in mental or emotional stresses, it was assumed that the new workers might get into some mental or emotional stress perhaps due to their first experience of the work at factory and adapted to such a stress after three months of work already.
The effect of quartz particles on the incorporation of C14-leucine and C14-glycine into rat intraperitoneal macrophage protein was studied. The incorporations of C14- leucine and C14-glycine were inhibited very rapidly and remarkably by alkali-treated quartz particles, but not by ground quartz particles. And the incorporation of C14- palmitic acid into cell lipid was also strongly inhibited by alkali-treated quartz parti-cles. The lactic acid production and the succinoxidase activity of the cells did not decrease by the addition of quartz particles under above experimental condition. So, it was assumed that the rapid decreases of incorporation of C14-leucine and C14-palmitic acid into the cell protein and lipid were the early sign of cell damage.
The author carried out the experimental studies on the preventive effect of copper on the nitroglycol poisoning. In mice administered subcutaneously daily 600 mg/kg of nitroglycol and 0.5 mg/kg or more of copper, the survival time was prolonged remarkably compared with the mice administered nitroglycol alone. But ceruloplasmin which is copper-protein in plasma was not effective to extend the survival period in nitroglycol poisoning as recognized in copper. The sensitivity increase of nitroglycol poisoned mice to administered adrenaline was inhibited remarkably by administration of copper for several days. It seems reasonable from these results to assume that copper plays an important role in the nitroglycol poisoning.
The enzymes which catalyzed the hydroxylation of benzene were extracted from the rabbit liver ground with quartz sand. When the enzymes extracted with water or 0.2M phosphate buffer solution (pH 7.4) were added into benzene solution with NADP and citrate, phenol was produced and all of it was found to be present in free state, while those extracted with 30% sucrose solution could produce conjugated phenol as well as free phenol without the addition of ATP.
The metabolic pathway of benzene was studied by using an enzyme sample extract- ed from the rabbit liver. The main parts of the pathway suggested by Williams were ascertained and moreover it was found that the metabolites of benzene showed consid- erable inhibitory effects upon the hydroxylation of benzene. As the result of the inhibitory action of the metabolites, the reaction curve in respect to the amount of phenol or catechol produced from benzene may becomes to show the wave form appear.
The metabolism of benzene was studied with and without the addition of ATP and K2SO4 by using the enzyme samples extracted from the rabbit liver. When an enzyme sample extracted from the ground liver with 0.88M sucrose solution was used, a con-jugated metabolite estimated as conjugated phenol was produced from benzene, but it was not from phenol. This conjugated metabolite was assumed to be conjugated dihy-droxydihydrobenzene and the conjugating capacity of this compound was found to be larger than that of phenol. Accordingly, it was concluded that the conjugating capac-ities of phenols produced from benzene were in the order of dihydroxydihydrobenzene, phenol, catechol and then hydroquinone.
The impact strain responses of the sphenoidal bone surrounding the optic canal were investigated and their frequency spectra were studied in order to analyse above impulsive response. Then, the direction of the fracture of the sphenoidal bone and the time course from the contact moment to the occurence of the fracture during the collision were also measured. The conclusion are as follows. (1) The resonance frequencies of the strain of the sphenoidal bone surrounding the optic canal are above 800c/s and below 3 kc. (2) The Q value of the strain spctrum is important to estimate the characteristics of the impact response in a similar way to the transient phenomena in the alternating electric current.
Strong acids are very effective to destroy the organic substances and they dissolve the metallic materials at the same time. The mixed acid of concentrated sulphuric acid and nitric acid or aqua regia is suitable for this purpose, and is used widely. But they are not always effective to dissolve the metallic elements. In this experi-ment, the solubilities of cadmium and lead or their oxides in the nitric acid are determined and the dissolving mechanisms of these two metals are found not to be the same. The solubilities of lead and its oxides have their maximum at some concentration of the acid, but those of cadmium and its oxide have not. In dissolu- tion of metallic substances, therefore, concentration of acid as well as its kind should be considered.
Humidity effect on Rf values and sizes of spots of polynuclear hydrocarbons separated by thin layer chromatography was studied with the following adsorbents and developing solvents ; aluminium oxide G with n-pentane : ether (19:1, V/V), silica gel WOELM with n-hexane : o-dichlorobenzene : pyridine (10:1:0.5, V/V). The humidity range studied was 3-76% in relative humidity. In thin layer chromatography of aluminium oxide G, the Rf values and sizes of the spots were remarkably affected by humidity and successful separation was obtained at relative humidity of abut 20%. In thin layer chromatography of silica gel WOELM, the Rf values and sizes of the spots were slightly affected by humidity. Separation of the hydrocarbons was satisfactory in the humidity range tested excepting very low humidity.
The portable Digital Dust Indicator was improved. The size of improved apparatus was 204 × 130 × 84mm, and its weight was about 2.5kg. As the electric power, 6 dry cells were used for the required power of 1.5 watt. The response of one pulse per minute corresponded to the dust concentration of 0.05mg/m3 for usual mineral dusts. It was possible to estimate by this apparatus the dust concentration from 0.05mg/m3 to 500 mg/m3. The relation between the response of apparatus and the dust concentration was expressed by the equation R= AC/p0.6 Some different points of this equation from the expression derived for the other type of Digital Dust Indicator (Type A or S) were discussed. The optical maze provided at the position of air-inlet of this apparatus truncated the particles larger than 20μ in Stokes diameter. The estimating method of the mass concentration of dust particles under 5μ in Stokes diameter was demonstrated.