The dietary record for two weeks from 15 women was arranged according todefined food items. The individual's average daily intake of each food item correlated with the mercury content in the red cell and plasma. A stepwise regression analysis was carried out. By correlation and stepwise regression analyses, only the intake of fish, mainlymarine, was found to be significant in determining the mercury level in the red cell and it explained 80% of the total variance. No significant correlation and regression coefficients were found for the mercury level in the plasma.
At the critical point of the oxygen consumption, oxygen tensions in the arterial blood as well as in the mixed venous blood and the oxygen supply to peripheral tis-sues were studied and the following facts were found out: 1) critical oxygen tensions in non-anemic dogs were about 30 mmHg in the arterial blood and about 24 mmHg in the mixed venous blood. At this critical point, envirnomental oxygen content and oxygen saturation of the arterial blood were estimated to be about 5% and 65% respectively, 2) critical oxygen tension shifted to higher values of oxygen tension with lowering of oxygen capacity of the blood, and 3) in the range below critical oxygen tension, total oxygen consumption was almost linearly correlated with a normalized oxygen supply into peripheral tissues regard-less of changes in the oxygen capacity of the blood.
Arginine was reacted with 1, 2-cyclohexanedione even under a mild condition. This reaction has been used for studying the relationship between the arginyl residues and the biological activity of hen egg white lysozyme. The seven of all the eleven arginyl residues of lysozyme were specifically modified with 1.2-cyclohexanedione at the weak alkaline pH for two hours. The modification did not affect against the hydrolytic activity toward glycol chitin, but abolished the lytic activity toward Micro-coccs lysodeikticus cells.
Marked effects of nitroglycol on superprecipitation of actomyosin and on the initial burst of ATP decomposition by actomyosin were observed in vitro experiments. Instantly after incubating actomyosin with 0.22000 μM of nitroglycol, both super- precipitation and the initial burst were suppressed, while after 24 hours' standing at 0°C, they were strengthened reversely. The reverse effect of nitroglycol on actomyosin was explained as being due to the structural changes of actomyosin in respect of α-helix content. In a previous study, we have found out that actomyosin prepared from poisoned rabbits differed from normal one. Probably actomyosin and thus muscle fiber also of poisoned animals may be in disturbed states in respect of its structure and function.
This study was performed for finding out early diagonosis methods of methyl iodide or methyl bromide intoxication. Methyl iodide introduced into living organism was found in the brain, especially in the cerebral cortex, and in the liver and kidney. Gait disturbance which is one of the serious effects of methyl iodide on the nervous system seemed to be an representation of susceptibility increase of the organism agaist d-tubocurarine as reported previously. At this intoxication state, a disturbed pattern of lipid metabolism in brain tissues was observed similarly as the metabolic distur- bance of energy rich phosphate compounds reported already. Previously, we have found out an abnormal increase of serum lipid in poisoned animals. This finding was also ascertained by examining men exposed to methyl iodide or methyl bromide. Present address, Japan Industrial Safety Association, 5-35-4, Shiba, Minato-ku, Tokyo.
Retained amount and concentrations of cadmium in the kidney, liver, bones, digestive tract and others were compared between non-pregnant and pregnant female mice after the subcutaneous injection of carrier-free 109CdC12 solution. Mice were maintained with the synthetic diet without any particular addition of vitamins A and D. The cadmium concentration in the kidney of pregnant became double what it is in non-pregnant mouse on the average, but that in the bone was less in pregnant than in non-pregnant mouse.
A hypothesis on the critical concentration of mercury in the brain, which is neces-sary to induce neurological symptoms, was tested again by feeding of methylmercury chloride to mice. As well as in the previous experiment; single or repeated peroral administration of methylmercury chloride to mice, death followed the occurrence of neurological symptoms, when the accumulated amount of mercury was more than 30 μg Hg/g. The most sensitive, namely earliest, symptom; the lessening of the head mainte-nance on horizontal position on being hung with the tail was noticed at a brain con-centration of 10 μg Hg/g. The lowest daily dose group of 10 μg Hg/g of diet did not show the loss of body weight and neurological symptoms during the observation period up to the 41st day of mercury feeding.
Identification of composition of organic solvents is very important for evaluation of industrial environment. Gas chromatographic and infrared spectrometric analyses are suitable to identify organic solvents in commercial materials. The volatile com-ponents in the solvents have been identified by gas chromatography. The identifica-tion is certified by infrared spectrometric analysis of gas chromatographic eluates. In some cases analysis by only gas chromatograph gives an erroneous result and it is generally desirable to identify each component eluted from chromatographic column by infrared spectrometry. These examples are illustrated by using commercial ma-terials including several solvents ATR method also is useful to infer the possible kinds of solvents used from the knowledge on non volatile substances in the materials.