Experimental transplantations of fresh, preserved and fixed auto-, homo- and heteroadipose tissues were conducted with animals in order to examine their applicability as tampon. The result obtained was as follows;
1. The hetero-transplantation of fresh tissue was not successful. In case of auto- or homo-transplantation, adhesion and transformation of the transplanted tissues into connective tissues took place in about six months after transplantation.
2. In case of homo-transplantation, preliminary preservation in Ringer's solution and the preservation for about 10 days was more preferable. Hetero-transplantation was impossible after any length of the period of preservation. 3. When fixation was made with Baker's formalin calcium chloride solution, homo-transplantation was often successful after about 30 days of preservation. Hetero-transplantation was also impossible with this method.
4. The capsules formed were thick in case the transplantation was made outside of the periosteum of ribs or in the muscle. In the former case, the transplanted adipose tissue usually became flat in shape.

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There are only few therapies of allergic diseases, and numbers of unspecific treatments are prevalently performed. We must discover drugs effective in allergic disorders through experiments with such drugs, investigating the process of effective drugs and the mechanism of allergy itself. In the previous report, the author made clear the anti-allergic functions of about 70 drugs such as antihistaminics, autonomous blocking agents, flavonoids and others. In the present paper, the author reports the results of experiments on anti-allergic function with guinea pigs in state of anaphylaxis caused by injection of horse serum. Results were as follows:
Antihistaminics such as chlorpromazine and histacur proved effective as well as EDTA and Tropin (Takeda). Adrenochrome (Tanabe) and Myanesin were found less effective. It is known that EDTA chilates Ca-ion and Mg-ion, and once these ions are chilated, complement is weakened with its activity. Therefore, anti-allergic function of EDTA will be proved by inactivated complement. Based on the above experiments and other experiments (not yet reported), the author would like to conclude that complement plays quite an important role in allergy. Incidentally, the author made investigation to see if these anti-allergic drugs would save mice from toxic death caused by vagostigmine. Its result was that these antiallergicas were found to have scarcely antagonistic effect in the toxic death of mice, and parasympathetic blocking agents were effective.

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Eighteen healthy guinea pigs were inoculated subcutaneously with 0.01mg of tubercle bacilli (H37Rv strain). On the 8th day after the inoculation, they were divided into 4 groups and treated as follows: Group I, treated by daily intramuscular injection of 2mg of streptomycin. Group II, treated by twice weekly subcutaneous injection of 0.25cc of antigène méthylique. Group III, treated with both streptomycin and antigène methylique in just the same way as in the preceding two groups, respectively. These procedures were continued for two months. All the experimental animals were autopsied for the examination of macroscopic tuberculous lesions of internal organs and lymph nodes and for the enumeration of the viable bacilli remaining in the lungs, livers and spleens quantitatively.
The results obtained were summerized as follow: In the group treated with streptomycin and antigène méthylique, macroscopic lesions were hardly seen and the viable bacilli were not found in the lungs, livers and spleens. The combined treatment showed more beneficial effects than the treatment with streptomycin or antigène méthylique alone.

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Since the flame-photometer became available, studies on electrolyte metabolism have been made in clinical fields and there have been a few reports on the electrolyte alternation in TB, but all of them are about the urinary and serum electrolyte abnormalities. In as much as the major part of body is consisted of bone and muscle, investigation of electrolyte alterations of them in TB may be of value, especially, if it suggests any close relationship between electrolyte metabolism of those and that of serum or urine. The author conducted investigations on the serum electrolyte concentration, urinary electrolyte excretion and electrolyte contents of bones and muscles in preoperativie cases of pulmonary TB and attempted to evaluate the correlation with the pulmonary functions. In the present study, 113 consecutive patients with pulmonary TB who were eventually subjected to surgical interventions were investigated in the following procedures ; Sodium and potassium concentrations were measured by the flamephotometer. Chloride and calcium were measured by Schales' and Sobel's methods, respectively. Bone specimens were taken from ribs excised at the operations and muscle specimens from the operative wounds. Results were as follow;
1. In those cases who had severe pathological findings of pulmonary TB, serum sodium and chloride concentrations were low, and bone sodium and calcium contents were low, although serum concentration, bone and muscle contents of K were at variance.
2. In the cases with decreased pulmonary functions, serum Na and Cl concentrations were low.
3. Marked decrease in bone Na and Ca contents were noted among those who had diminished maximal breathing capacity and also those accompanied by marked pleural fibrosis.
4. Both bone Ca and Na contents decreased or increased in parallel. It was also found that bone atrophy was always associated with a decrease of bone Na contents.
5. Those who had severe pulmonary TB showed hyponatremia as well as decrease of bone Na contents, this may be coincidental observation.
6. No relationship was found between Na and K contents in muscle and the degree of pulmonary TB or the ventilatory functions of lungs.

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In various cases of pulmonary tuberculosis, the author conducted measurements of electrolytes in serum and urine before and after the administration of adrenocortical hormone. Results were as follow:
1. In mild cases, no remarkable change was found, but in medium or severe cases, especially in active cases, Na, Ca and Cl became low, K high, i, e., smaller Na/K and larger K/Ca.
2. With the injection of Rindex (1cc per 10kg), no remarkable change was observed in mild cases, but in medium cases Na in serum increased a little, K decreased slightly, i, e., Na/K became larger, K/Ca smaller. In serious cases both Na and Cl increased and Ca slightly, while K decreased, i, e., Na/K became larger, K/Ca smaller. Variation in urine of medium cases was almost similar to that in serum, while mostly in severe cases it was not. Those variations disappeared in 180 minutes after the injection.
3. By subcutaneous injection of Rindex (1cc per day for 2 weeks successively), variation in serum of mild cases was very slight, and in medium cases, Na and Cl increased, K decreased, and as for Ca the results were diverse, i, e., Na/K increased and K/Ca decreased. In serious cases, Na, Cl and Ca altogether increased, while K decreased, i. e., Na/K increased and K/Ca decreased. This variation was most remarkable in serious cases, and it recovered by 10 days after the final injection. In the urine of mild cases, Na decreased by the injection while K increased, i, e., Na/K decreased. In medium cases, Na, K, Cl altogether decreased, but Na/K was diverse. In serious cases, Na, K and Cl increased and K began to decrease 7th day and 14th, i, e., Na/K decreased by 7 days and recovered by 14 days. Thus the variations in urine were diverse. Perhaps that is due to the differences in the quantity of water taken, function of kidney, and circulating system, togetherwith quantity of perspiration and kinds of food taken in each cases.

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The present paper deals with the variations of migration velocity of neutrophile leucocytes and the peripheral blood picture after thoracoplasty or lobectomy.
1. Migration velocity after the 1st and 2nd thoracoplasty underwent transient marked decrease. However, it recovered pre-operational level by 10 days after the 2nd operation. After that it exceeded the pre-operational level.
2. Almost concurrently with the decrease of migration velocity the peripheral blood picture fluctuated greatly, i, e., transient decrease of erythrocytes, increase of leucocytes, lymphopenia and neutrophilia were observed. These recovered mostly one month after the 2nd operation. There after, accompanying the increase of migration velocity, the percentage of lymphocytes markedly increased and kept high level for 3 months.
3. All the variations stated above were more marked after the 1st operation than after the 2nd one.
4. After lobectomy the migration velocity gradually decreased. It was smallest on the 3rd day and after that it began to recover. In most cases, the pre-operational value was restored by 3 weeks.
5. The variation of the blood picture, after lobectomy, was most marked immediately after the operation. It slightly preceded the variation of migration velocity and it almost recovered its pre-operational value by 2 weeks.

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