By employing Takeya's Fatigue-reaction method, and Zambrni Watanabe's Salivary-reaction method, the authors carried out an investigation of fatiguability caused by surgical operation. They further studied the effects of amino acids upon fatiguability incident to surgical procedure
Results are as follow:
1) No marked difference was noted in the reactions between the urinary and salivary methods. However, a stronger and more lasting reaction was observed in cases of intra-abdominal operation as compared with extra-abdominal operation.
2) The fatigue-reaction was found to be weaker and recovery rapid in cases in which injections of amino acids were given than those in which no such treatment used.

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The sensitive strains, SM- or Pc- resistant variants and reverse mutants of Shigella dys-enteriae, 16 strains, were compared with each other on the biological characteristics. The sensitivities of 16 parent strains to SM ranged from 2.5 to 10γ SM per ml, to Pc from 3.5 to 60 units Pc per ml. The SM-resistant variants of these strains which were repeatedly cultured on the agar plate containing SM for fifty days grew in 10, 000γ of SM per ml. The Pc- resistant variants of these strains which were repeatedly cultured on the agar plate con-taing Pc for fifty days grew in cocentration from 250 to 1000 units of Pc per ml.
It is the reverse mutants that the resistant variant was repeatedly cultured on the agar plate free from SM or Pc for fifty days. In the case of SM the sensitivies of 12 reverse mutants were equal to that of the SM-resistant variants, 3 were sensitive to 600γ of SM per ml, the remaining 1 strain was sensitive to 150γ of SM per ml. In the case of Pc all the reverse mutants were equal to the parent strains in the sensitivity to Pc.
Morphorogically the form of the SM- or Pc- resistant vaiiant changed from bacilli form to round form on the agar plate containing SM or Pc.
In growth rate the resistant variants grew later than the parent strains in the cases of both SM and Pc.
In the other biological characteristics, such as the cleavage of carbohydrates by bact-eria, coagulation of milk protein etc, no visible difference was observed, and the prolongation of all the enzyme actions were due to being slowness of the growth.
The SM-resistant variants grew better on the agar plate in the presence of SM than in the absence of SM.

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A radiographic sudy for various parts of the body was made by employing a rotato-transeverse radiographic apparatus which was improved and constructed by the author.
Following are the results of the study:
1) A desired level of the body structure may be brought out on the film eliminating superimposition of other structures.
2) The perspective may be made distinct as only the contour at a certain level of the transverse section is delineated.
3) The shape, the size and the localization of the lesion may be brought out on the film by the combined use of simple-radiogrphy.
4) The technique of this method is simple, the image clear, and the cost reasonable.

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The author studied the life span of 12, 000 patients who had died in the Nakano National Sanatorium during the past 20 years (1928-48). On comparing the statistical Dx-curve with the function of age-distribution which was proposed by Kawakami, he confirmed approximate agreement between them.

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