I have studied on the relationship between the forms of tubercle bacilli in the bronchial secretions and some clinical findings of pulmonary tuberculosis.
The results obtaind are as follows:
1. The forms and structures of tubercle bacilli in the bronchial secretions prove to be polymorphous.
The long-shaped bacilli, chain-shaped bacilli, bind-shaped bacilli, indefinite-crowded baci-lli and granular-shaped bacilli in the bronchial secretions do not show much difference from those of the sputum, but in medium-sized bacilli and short bacilli showed some difference.
2. The prognosis of pulmonary tuberculosis considered in relation to the forms of tubercle bacilli, seems to be good when increased number of long bacilli were found in the bronchial secretions, and short bacilli seem to be somewhat increased among the patients who are getting worse.
Long bacilli are often discovered in the bronchial secretions of the patients with mixed-type roentgenographically, and in those with the normal erythrocyte sedimentation rates short bacilli were more numerous.
3. The increased number of so called mass-shaped bacilli in the bronchial secretion seems to be found in the patients with cavity in the lung.

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The bacteriologic characteristics of 25 strains of human type tubercle bacilli, obtained by our method as reported in previous paper were studied. Followings were the results of our experiments.
1) Morphology and Staining: Non acid fast, gram negative, non motile, non spore-bearing, non capsulated rods, variable in size ranging from 0.5 to 2.S in length by 0.3 to 0.8μ in diameter. By method of Ziehl-Neelsen in most strains one or more deeply blue str-ained granules were seen. The majority of the strains were elogated, irregular and somew-hat centrally arched. In older cultures even plemorphism was often observed.
2) Cultural characteristics: The organisms were cultivated aerobically at 37°C and could be grown on the ordinally media, but all of them multiplied best on those used for culturing tubercle bacilli. In liquid media, growth developed usually at the bottom of the tube but the strains of T z group showed an uniform growth throughout. Penicle formation was noted occasionally. In the mist favorable medium, colonies were detected in two or three days Maximum development took place in 5-7 days,
3) Colony morphology: Convex, smooth, shiny and soft to tanacious in consistency. In color the strains of T_I group ranged from white to grayish-white, of T_II group from red to purple red, and of T_III group from lemon-yellow to orange. Pigmentation was found inc-reasing with the age of the cultures and the pigments did not diffuse into media. Colonies were easily emulsified in saline.
4) All of the strains produced no gas, formed no indol, did not reduced nitrates, did not liquefy gelatin, and coagulated milk.
5) Most strains produced acid with Glucose, Galactose, Fructose, Lactose, Xylose and Arbinose but not with Mannose, Maltose, Saccharose, Dextrin and Rhamnose.
6) The action of Oxydose and Urease of the mutated strains was found to be alike to that of tubercle bacilli.
7) In spite of the above discrptive characteristics of the mutated strains, the identific-ation of the organisms was still difficult but in view of the fact that tubercle bacilli may have a cycle of mutation on their own way, they could be differentiated from so called non pathogenic acid fast bacilli etc.

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In this series of experiment on morphological observation of Tbc bacillus, both in vitro and in sputum, over one year, under the administration of TB-1 was undertaken, i. e. the influence of TB-1 was carefully observed in respect to the length of bacilli, the numbers of granule, K. F., change of morphology, and attitude in staining. And the results are summarized as follows:
1) In vivo experiment, human strain H37 RV was cultured with increasing concentrat-ion of TB-1, on the culture media of modified Proskauer-Beck, Kirchner, Oka-Katakura. And as the result, there was shortening of the length of bacillary body, increase of granules, lessened K. F., in proportion to the concentration of TB-1.
2) Under the TB-1 administration, the length of bacillary body in sputum showed shor-tening in early stage, which was followed by the tendency of elongation; granules were increased without exception and K-F seemed to decrease a little, however not so remarkable.
3) There is a tendency of gradual decline in TB-1 sensitivity of this micro-organism in the course of time and a certain corelation was seemed to exist between the change of the length of bacillary body on one hand and the TB-i sensitivity and the clinical effect of the drug on the other, though this finding is not yet conclusive due to the scarceness of the observed cases.
4) In addition too these findings, it was noted in a certain extent, both in vitro exper-iment and in sputum: the morphologiecal change of bacillus, the increase of Ziehl-granule, the lessening of acid-fastness, the change of attitude in staining etc., of which however, author would not think much stress should be layed upon.

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179 cases of temperature charts of inpatients and left patients were investigated for this studies. We made two fever curves from the original fever curves in which fever was taken four times in a day, at 6 and 10 o'clock a. m. and 3 and 6 o'clock p. m.. In the first curve, called A curve, fever at 6 a. m. and 3 p. m. was abstracted. In the second curve, called B curve, fever at 10 a. m. and 6 p. m. was abstracted. Comparing these two curves, we reached following results.
1) B curve was more similar to original curve than A curve.
2) Even if B curve was not similar to original curve, we may not make large mistake on the diagnosis and treatment refering to other subjective and objective symptoms.
3) By strict studies on fever curve of 172 patients, we found that most of them showed the highest fever at 6 o'clock p.m.
4) In Tb-sanatorium, two times fever taking system (at eight thirty in the morning and six in the evening) is sufficient for usual treatment, but we must take fever more frequently, only when the fever curve is abnormal.

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