It has been found that low energy laser exposure can affect (biostimulate) various processes in cells and tissues. The effects of direct He-Ne laser on the histopathology of a primary culture of satellite cells isolated from rat skeletal muscle was investigated using electron microscopy. The cultured cells were directly exposed to laser light for 3s, and were prepared for electron microscopy 4 days post-irradiation. Non-laser-irradiated (NLI) cells served as a control. The treated cells exhibited well-developed rough endoplasmic reticulum, mitochondria and other cell components, similar to the NLI satellite cells. Previously, we showed that the same dose of laser therapy enhances satellite cell proliferation twofold. Taken together, we suggest that direct He-Ne laser treatment at the power density that can enhance satellite cell proliferation in culture does not cause histopathological changes in the cells, as manifested by their ultrastructure.
We studied the effects of low intensity lasers on the production of reactive oxygen species by phagocytes in vitro, in order to explore some of the therapeutic mechanism of laser photostimulation. Rat alveolar macrophages (AMs) were treated with various doses of 830 nm laser before and after incubation. We used lucigenin-dependent chemiluminescence and the cytochrome C reduction method to determine superoxide generation and NADPH oxidase activity, respectively. After incubation, laser treatment (LT), using 60 mW for 10 or 15 s, or 80 mW for 15 s, significantly accelerated peak time (PT) of chemiluminescence without any significant effect on peak height (PH) (P < 0.05, P < 0.001, and P < 0.01, respectively). In contrast, treatment with 150 mW LT for 5, 10 and 15s significantly delayed the process (P < 0.05, P < 0.001, and P < 0.01, respectively). Furthermore, LT with doses higher than 60 mW suppressed NADPH oxidase activity before incubation. Treatment of the macrophages with 30 mW for 10 or 15 s, 40 mW for 15 s, and 60 mW for 5 or 15 s significantly accelerated PT (P < 0.05 in each case). Similarly, there was a significant increase in PH when AMS were treated with 60 mW for 10 s or 80 mW for 10 or 15 s (P < 0.05 in each case), but these dose levels had no significant effect on NADPH oxidase activity. These results indicate that laser photostimulation can modulate AM function, and that the effect does not depend on the total amount of energy applied, i.e., the output power multiplied by the treatment time. Furthermore, our results show that 60 mW is a suitable output power for activating AMS.
We studied the changes occurring in the metabolism of proteins (serum cholinesterase, total proteins) and glycosides (serum glucose) in Cavia cobaia. The result of this simple blind study was evaluated on the first, tenth and twentieth days of treatment. A Duncan test was used for the graphical and statistical analysis of data. The technique and treatment doses were similar for all groups, laser treated, red light treated, and controls. In the groups treated with He-Ne laser, serum cholinesterase levels increased proportionately with the dose, reaching a peak on day 10, which was not the case with the controls. Monochromatic red light caused a similar but quantitatively lower effect. The same results were obtained in the case of serum proteins, however the effect did not depend on the dose, and it was less statistically significant than in the case of serum cholinesterase, both in laser treated and monochromatic red light treated groups. Serum glucose levels increased after He-Ne laser biostimulation in inverse proportion to the dose. The same effect was obtained in the case of monochromatic red light biostimulation. Serum glucose levels decreased in the control groups. He-Ne laser and monochromatic red light biostimulation seem to cause an increase in the levels of serum cholinesterase and proteins, and a general stimulation of protein metabolism. Serum glucose levels also increased after both techniques - inversely proportionate with the dose - whereas in the control groups it decreased during the experiment.
The aim of the international Cochrane collaboration is to continuously evaluate new and old medical therapies. The basis for the systematic reviews is the recognition of Randomised Controlled Trials as the “gold standard” for scientific evaluation of small and moderate effects from treatment. A thorough search is made for the available literature and the most “qualified” studies are analysed. The purpose of the analysis is to find out whether or not there is any solid support for a specific medical treatment modality. Such analyses are published in medical journals and extended versions are quarterly updated in the Cochrane Library. Since the Cochrane reviews are influential, it is important that the persons performing them possess a thorough knowledge about all aspects of Low Level Laser Therapy. The aim of the present study is to “analyse the analyses”.
The Achilles tendon, although the largest and strongest tendon in the human body, has been recognized as a weak point since classical times. In the case of Achilles, an arrow severed the tendon, but repetitive body load is what causes this problem for athletes. The whip effect of pronation in the running gait and potential intrinsic weakness or collagen deterioration with age makes the Achilles tendon very vulnerable. Poor results with conservative therapy and laser therapy (LT) prompted us to use a combination of LT and local infiltration with concomitant medication. The subjects were 100 successive athletes with chronic Achilles tendinopathy who had undergone conservative treatment for six months without positive results. This study used a combination of Photodynamic Therapy with Plenosol® intradermal and 660nm LT, followed by a cooling down treatment using Ubiquinon, Ferrum and Cu+ + subcutaneous infiltration, LT with 660nm, 830nm and 904nm. After an average of 4 treatments in 14 days, healing was achieved in 16 days and training was resumed in 90% of the patients. With these results, it was concluded that a good knowledge of the interaction between laser and pharmacology could reduce treatment time, even for chronic cases of Achilles tendinopathy.