Skin rejuvenation via the nonablative approach remains a hot topic, but the results have been disappointing with single modality treatment. The combination of two different wavelengths, 1064 nm from the Nd:YAG laser and an IPL broad waveband with a 570 nm cut-off filter were combined to assess the results, and the strong contributory role of athermal photobiomodulation was carefully considered. Forty female patients (11 forehead, 14 periocular and 15 perioral), ages ranging from 27 - 50, skin types II - IV, wrinkle types I - III participated in the study. The IPL system was applied with the yellow (570 nm) cut-off filter, 30 J/cm², single shot, followed by the Nd:YAG at 120 J/cm² , double shot (7 ms per shot with 20 ms between shots) on the wrinkled areas only. Three sessions were given at monthly intervals, and an assessment was made one month after the third session, following which another treatment was given, and the patients followed for 6 months. Biopsies were taken from 4 consenting patients as a cross-section before the first treatment, one month after the 3rd session and at the 6 months point, to help assess collagen production and remodeling. The histology showed thickening of the epidermis with good dermal collagen organization. Thirty-seven patients completed the entire study protocol, with an overall satisfaction index (SI) of 20 (54%) at the 1st control point and 30 (81%). at the 2nd control point, showing the long-term contributory role of the athermal photobiomodulation zone accompanying the zone of delivered thermal damage. The addition of the Nd:YAG laser to the IPL regimen in nonablative skin rejuvenation gave very good histological results which were echoed in strong patient satisfaction with the visible improvement in their skin condition. The role of the various photoactivated skin cells in the photobiomodulation was deemed extremely important to the final good result, coupled with the potential role of heat shock protein

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Many pharmacological treatments for Alzheimer disease (AD) exist, including treatment with reversible cholinesterase inhibitors which has improved cognition and slowed progression of dementia in some patients, as low levels of acetylcholine are regarded as one of the potential causes of this disease. High levels of the β-amyloids are regarded as another possible contributing factor. Laser therapy and magnetic field therapy have shown promise, singly and, much better, together in reversing these two potential AD-related conditions. The present study examined polylaser therapy on its own and combined with magnetic field therapy in 42 patients diagnosed as having AD, in whom the activity of cholinesterase in the blood significantly decreased compared with the initial levels, and simultaneously the concentration of acetylcholine and cholinergic index were enhanced. A noticeable effect was achieved in more than half the time in the patients treated with the combination therapy, compared with polylaser alone. A control group of 22 non-dementia patients with familial mediterranean fever (FMF) complicated with amyloidosis were treated with polylaser together with plant-derived medicines (phytoceuticals), to assess the potential effects of this regimen on the amyloidosis component of FMF. In the 18 patients with grade I-II FMF, complete regression of the amyloidosis, proteinuria and improvement of kidney function were achieved. In the remaining 4 patients, grades IIa-III, no such improvement was detected. Combined therapy with polylaser and magnetic field energy coupled with the use of appropriate phytoceuticals my well offer an interesting ine of approach in the treatment of ADE, in addition to helping solve the problem of finding a successful treatment of FMF

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We previously constructed the cDNA library of osteoblastic cells using a stepwise subtraction procedure between cells with and without laser irradiation to identify any gene whose expression is enhanced by low level laser irradiation (LLLI). In the present study, we focused on a gene clone obtained from the subtracted cDNA library, designated as #201, which exhibited high homology with the gene coding for F₀F₁-ATPase subunit-b which is involved in the synthesis of ATP. To confirm the gene, the DNA sequence of the cDNA clone #201 was determined and assessed in DNA databases. The transcription level of the gene was examined by Northern blot analysis. Furthermore, the intracellular ATP content in laser irradiated cells was measured and compared with unirradiated cells. The DNA sequence of #201 clone exhibited 94.9% and 94.1% homology with rat and human F₀F₁-ATPase subunit-b gene, respectively. Higher F₀F₁-ATPase subunit-b mRNA levels were observed in laser irradiated cells compared with those in unirradiated cells. Furthermore, the content of ATP in laser irradiated cells was higher than that in unirradiated cells. These findings suggest that LLLI may play a principal role in accelerating ATP synthesis by stimulating the gene expression of F₀F₁-ATPase.

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The mast cell in an interesting repostory of a number of pro- and anti-inflammatory mediators, and plays an important role in the inflammatory stage of the wound healing process, and in allergic reactions. The present study was designed to elicit the effect of visible light radiation at 632.8 nm (visible red) on mast cells in vivo using light and transmission electron microscopy. The tongue of the Swiss mouse is rich in mast cells (MC_s). Tongues of animals in two experimental groups were irradiated using a HeNe laser (15 mW & 4 mW, 2.4 J/cm²,for both output powers, λ = 632.8 nm), and a third set of unirradiated animals served as the control. The two output powers were used, with the irradiation time adjusted to produce the same energy density. The possible nonselective degranulation of LLLT-irradiated mast cells was examined quantitatively and morphometrically using optic and electron microscopy. By means of radioimmunoassay, the histamine content of pulverized tongue was evaluated, and the histamine levels in both experimental and control tissue were compared. The irradiated tongue tissue showed a significantly higher histamine level compared with the control. In addition, the level of histamine in the interstitial cellular medium and the degree of other histological changes such as vasodilation was significantly higher following a single irradiation with the HeNe laser at the above settings. The observations from this study may serve as a further indication of the bioactivative effect of low reactive level laser therapy on mast cells, which are an important component of the wound healing process and inflammation control.

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Infertility is a major physiological and psychological problem for women, and can also put strain on the husband-wife relationship. A number of approaches have been developed for the infertile woman with a fertile husband, such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and artificial insemination with the husband’s (or partner’s) sperm (AIH). Although the success rate with these approaches is good, some women remain refractorily infertile. Low incident levels of laser energy, known as low reactive-level laser therapy (LLLT), have been proved useful in a variety of clinical fields, including the enhancement of local blood flow rate and volume. The present study evaluated the adjunctive application of an 830 nm diode LLLT system (60 mW in continuous wave, 5 min/point, approx. 300 J/cm²) in combination with artificial reproductive technology (ART). Twenty-four patients (ages ranging from 30-48 yrs) received LLLT, among whom there were 8 pregnancies (33.3%) and 3 miscarriages (12.5%), with greater efficacy seen in the younger age groups. Of the 24, 19 underwent ICSI plus LLLT with 7 pregnancies (pregnancy rate of 36.8%). On the other hand, in twenty-one ICSI patients during the same period who did not receive LLLT, only 4 pregnancies occurred (pregnancy rate of 19%). However, the series is too small to state definitively that LLLT significantly contributed to improvement in the rate of pregnancy among refractorily infertile women, but based on our experience we feel that LLLT improved the blood supply to the ovum and womb, thereby increasing the pregnancy rate. Furthermore, LLLT is safe, painless, easy to deliver and well tolerated. We will certainly continue using LLLT in infertile women, and larger studies are planned for the future to confirm the interesting results of the present study.

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