Background: The surgical management of varicose veins has been subject to radical change due to both the effective use of endovenous laser therapy (EVLT) and greater knowledge regarding the venous haemodynamics of the lower limbs. Aim: The aim of our study was to evaluate the results of varicose vein treatment using EVLT. Based on our results we discuss the use of laser, the appropriate use of crossectomy (a ligation and dissection of the great saphenous vein and its tributaries), and deduce the amount of energy required for the occlusion of treated veins. Method: 326 subjects treated with EVLT using the 810 nm laser. Results were retrospectively evaluated. Patients were followed-up with Doppler US at 1 and then 3 weeks, and 6 and 12 months post procedure. Results: Long-term complications were experienced by 11 (3.4%) patients in our subject group whilst re-canalisation occurred in 12 patients. Complete vein recanalisation was not detected. Conclusion: Based on our experience, crossectomy should be performed, although not routinely, in all patients suffering from varices of the lower limbs. We find the application of over 40 J of energy per centimetre of vein length (J/cm) avoids the possibility of burns. The application of such energy will allow safe occlusion of the vein with minimal risk of recanalisation and burns. Before the operation, we recommend the length of the treated vein be measured in order to deduce the minimum amount of Jules that should be used for a successful occlusion.
Background: The purpose of the current study was to investigate the possibility of rejuvenating red blood cells (RBC) and prolonging their life using low-level laser radiation (LLLR). The possibility that blood could easily be collected and preserved for 21-42 days determined the authors’ attempt to answer such questions as: the minimum LLLR dose capable of influencing blood qualities without damaging effects, the optimum characteristics of the irradiating source and protocol of irradiation. Because of its complex composition, the physical and chemical properties of blood have not yet been entirely elucidated. Blood irradiation additionally involves a special physical agent, as well as mechanisms of action that are not clearly understood. Material and Methods: We irradiated blood stored in MacoPharma bags, from seven healthy adult donors. A HeNe laser (6 mW, continuous wave) was used as the source of irradiation. Twenty-four to 48 hours after irradiation blood samples underwent rheological and biochemical analyses in order to establish the morpho-functional status of the red blood cells. Results: Marked effects occurred in the case of effectively received doses of 0.4 and 1.2 J·cm-3, suggesting a beneficial action of LLLR on the morphologically functional erythrocytes. Conclusion: This study demonstrated that blood rejuvenation by the physical method of laser irradiation could be an efficient, simple and economic procedure, and that it needs to be tested on patients who require urgent transfusion. Because the irradiated blood demonstrated a better capacity than the non-irradiated blood, we concluded that LLLR had a revitalizing effect on preserved blood.
Background and Aims: During the lifespan of the female, biochemical changes occur in the ovarian environment. These changes are brought about by numerous endogenous and exogenous factors, and can have a profound effect on ovarian oocyte quality. Oocytes of poor quality may be the cause of female infertility and are a major obstacle in successful assisted reproductive technologies (ART). We have previously reported on the efficacy of low reactive laser therapy (LLLT) using a GaAlAs diode laser device which emits laser light of 830 nm at an output of 60 mW to improve the poor quality of oocytes as an adjunctive treatment of refractory female infertility. The aim of this study is to assess the LLLT curative effect. Subjects and Method: Eight hundred and fourteen female patients who presented at the Ohshiro clinic for treatment of infertility from July 1st, 2000 till December, 2009 were enrolled in the analysis. We analyzed the therapeutic frequency and the therapeutic period (the number of menstrual cycles) between pregnant women and non-pregnant women. Results were expressed as means ± standard error of mean. The statistical difference was determined with the two-sided Student’s t-test and the Mann-Whitney’s U test. Differences with P ‹ 0.05 were considered significant. Result: The mean therapeutic frequency and mean therapeutic period in the subjects who gave birth was 3.3 ±2.6 times a month and 5.2 ±6.1 months, respectively, which correspond to the whole oogenesis period. Conclusion: We concluded that laser therapy once per week and a period of 5-6 menstrual cycles might be necessary to gain a good curative effect as the laser effect could last 1 week and the competence of oocytes depends on numerous processes taking place during the whole oogenesis period.
Background: Rheumatoid arthritis (RA) is a systemic autoimmune disorder that involves inflammation and pain of joints. Low-level light therapy (LLLT) is being evaluated for treating RA. However, the molecular basis mechanism underlying the effectiveness of linear polarized near infrared light irradiation (LPIL) is unclear. It has been reported that interleukin 1β (IL-1β) plays a key role in the progression of RA. Aim: The objective of this study was to determine whether LPIL (Super Lizer™; SL) decrease gene expression and production IL-1β in human RA synovial cells, MH7A, and RA joint tissue of collagen-induced-rheumatoid arthritis (CIA) rats. Materials and Methods: IL-1β challenged MH7A and CIA rat joints were irradiated with SL. Total RNA was isolated from MH7A, and gene expression profiles were analyzed using DNA microarray.The mRNA levels were confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. The production of IL-1β in rat knee joints was analyzed by immunohistochemistry. Results: DNA microarray analysis showed that IL-1β increased IL-1β gene expression in MH7A, and SL significantly reduced its IL-1β mRNA level. The reduction of IL-1β mRNA level by SL was successfully confirmed by reverse RT-PCR and real-time PCR. SL reduced the swelling of CIA rat knee joints, and the immunohistochemical study demonstrated that a strong IL-1β staining in synovial membrane tissue of CIA rat joint, and the immuno-staining was significantly reduced by SL. Conclusion: Since autocrine IL-1β production has been identified to be an important proinflammatory cytokine in the pathogenesis of RA, the reduction of IL-1β expression in RA synovial cells is one of mechanisms in reduction of the inflammation in RA by SL.