Background and Aims: Allergic rhinitis annually reach epidemic proportions in Japan. Approximately
30 to 40% of the population suffers from allergic rhinitis during the spring season. Symptoms comprise
rhinorrhea, nasal congestion, and sneezing accompanied by irritation and itching of the eyes. The Ohshiro
Clinic started using the conventional Nd:YAG laser for the treatment of allergic rhinitis in 1993, and
from 2005 we started using a diode laser-pumped Nd:YAG laser. From 2010, we adopted a novel 810 nm
diode laser, and the present retrospective study examined the efficacy rate of the treatment of allergic
rhinitis in the 2018 season with this system, compared with a previous study in 2011. We aimed to confirm
the degree of improvement for each symptom to evaluate effectiveness of the diode laser treatment. Subjects and methods: Between January 8, 2018 and April 30, 2018, a large number of patients consulted our clinic with the major complaint of seasonal allergic rhinitis. They underwent a blood test, and the
antigen-specific serum IgE antibody titers were measured for a definitive diagnosis of cedar pollinosis. A
total of 211 target patients were treated during the trial period. The average age of the target group was
36.3 years, 134 males, and 77 females. The target patients were treated with lower nasal turbinate mucosal
irradiation using a diode laser (ADL-20, Asuka Medical) delivering 810 nm at 7.5 W, with a total energy
per treatment of 240 J/cm2. We adopted a five-step evaluation in accordance with the Japanese Guidelines for Allergic Rhinitis 2014 for the symptoms of rhinorrhea, sneezing and nasal obstruction. We assessed the degree of improvement in the severity of these symptoms following diode laser treatment from baseline
to one month after treatment, in addition to assessing patient satisfaction with the degree of improvement
in their quality of life (QOL). Results: The 211 patients positive for cedar pollinosis by the antigen-specific serum IgE antibody tests were broken down by month by number and by improvement, no change or exacerbation as follows. January, 18 patients: 33.4%, 44.4% and 22.2%, respectively. February, 29: 10.4%, 44.4% and 22.2%, respectively. March, 146: 60.3%, 31.5% and 8.2%, respectively. April, 18: 77.8%, 16.7% and 5.5%, respectively. The monthly respective improvement, no change or worse patient QOL as percentages were as follows: January:
16.7%, 44.4% and 38.9%. February: 17.3%, 13.8% and 68.9%. March: 61.6%, 29.5% and 8.9%. April: 94.4%, 0.0% and 5.6%. The values for prevention of exacerbation versus exacerbation for each month were: January, 77.8% vs 22.2%; February, 41.4% vs 58.6%; March, 91.8% vs 8.2%; and April, 94.4% vs 5.6%. The mean efficacy rate for the trial period in the present study was therefore 52.6% which compared very favorably with the mean efficacy rate in the 2011 study of 53.4%. Conclusions: The results showed that the 810 nm diode laser offered a safe and effective solution for the uncomfortable symptoms of allergic rhinitis and could be well applied during the season of Japanese cedar pollen dispersion. /urthermore, a tendency towards high efficacy was demonstrated for laser treatment in class 6 cedar pollinosis patients, based on the specific IgE antibody test.
Background and Aims: Er:YAG laser has become optional for enamel and dentin preparation for a decade.
However, it is unclear if Er:YAG laser is acceptable for enamel conditioning prior to an application
of a pit-and-fissure sealant. This in vitro study thus aimed to investigate shear bond strength of a sealant to enamel etched with Er:YAG laser, as well as to demonstrate an alteration of enamel surface after the
laser was applied. Materials and Methods: One hundred and twenty extracted human premolars were divided into 4 groups (N = 30 per group) in which the enamel surfaces were treated with 1) 37% phosphoric acid, 2) Er:YAG laser 80 mJ/pulse, 2 Hz, 3) Er:YAG laser 120 mJ/pulse, 10 Hz, and 4) Er:YAG laser 140 mJ/pulse,
2 Hz. Prior to sealant application. Shear bond strength was determined by using a universal testing machine.
Statistical analysis was performed using One-way ANOVA. Modification of enamel surface after laser ablation was also investigated using scanning electron microscope. Results: Phosphoric acid-etched enamel yielded the highest shear bond strength when bonded with a sealant. Ablation of enamel with Er:YAG laser did not significantly improve sealant bonding since it demonstrated lower shear bond strength, compared to acid-etched enamel. Despite the presence of cracks after Er:YAG laser application, dissolution of enamel substances was limited. Conclusion: Our study has shown a reduced shear bond strength of dental sealant to an Er:YAG laser-irradiated enamel, compared with phosphoric acid-etched enamel.
Background and Aims: The emergence of drug-resistant infections is a global problem. Acinetobacter
baumannii has attracted much attention over the last few years because of resistance to a wide range of
antibiotics. Applying new non-antibiotic methods can save lives of many people around the world. Antimicrobial
photodynamic therapy (aPDT) technique can be used as a new method for controlling the infections. In this study we investigated the effect of aPDT on the expression of pathogenic genes in colistin-resistance A. baumannii isolated from a burn patient. Materials and methods: The suspension of colistin-resistance A. baumannii was incubated with 0.01mg/ml of toluidine blue O (TBO) in the dark; then the light emitting diode device with a wavelength of 630 ± 10 nm and output intensity of 2000-4000 mW /cm² was irradiated to the suspension at room temperature. Subsequently, after the aPDT, genes expression of ompA and pilZ was investigated by using real-time polymerase chain reaction technique. Results: Among the genes studied, the transcript of the ompA gene after aPDT was increased significantly in comparison with control groups (P ‹ 0.05). Whereas, there was no remarkable different in pilZ gene expression (P › 0.05). Conclusions: It can be concluded from the results that the ompA as an outer membrane of A. baumannii is degraded after exposing aPDT and it will probably be done the penetration of antibiotics into cells of this bacterium easily.