The effects of testosterone administration on the Iocalization of the androgen receptor and on the activity of nuclear phosphorylation were investigated in female mouse submandibular gland. Administration of testosterone (500μg/100 g body weight) to female mice caused translocation of androgen receptor from the cytosol to the nucleus within 3hrs. Approximately 80% of the nuclear receptor bound to the active chrornatin which was prepared from the nucleus by micrococcal nuclease digestion. Incorporation of ³² P from [γ-³²P] ATP into the nucleus was enhanced 3hrs after testosterone administration and then the enhanced incorporation returned to acontrol level in 6~9hrs. Such a phosphorylation activity was found in the active chromatin and asimilar effect of testosterone administration was observed on ³²P incorporation. Nonhistone proteins, which were extracted from the ³²P-labeled nucleus with 0.35 M NaCl, were mainly phosphorylated. These results indicate that mouse submandibular gland contains protein kinases which are activated by the nuclear androgen receptor, and that the kinases seem to be lnvolved in the transcription activation modulated by the androgen receptor.

Denture fibroma comonly exists, but extensive excision offen results in deterioration of the maintenance and stability of the denture. Excision of the whole tissue is not always nessesory, since fibroma is not a true tumor but is an inflammatory neoplasm. Therefore this tumor must be excised conservatively for the maintenance and stability of the denture. On two cases of denture fibroma, we performed excision of only connective tissue and vestibuloplasty simultaneously with preservation of the overlying mucosal membrane. Post-operatively, the den-tures retained stability.