岩手医科大学歯学雑誌
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27 巻 , 3 号
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研究
  • 大平 明範, 佐藤 理恵, 村田 尚子, 関山 三郎
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 157-165
    公開日: 2017/03/21
    ジャーナル フリー
    Based on arthroscopic findings in patients with temporomandibular joint arthrosis, the present study divided the synovial membrane in the upper joint cavity into anterior and posterior sections in relation to the articular disc. The relationship between temporomandibular arthralgia and site of synovitis was investigated. Temporomandibular arthralgia was assessed using the Visual Analog Scale, and severity of arthralgia during resting, mouth opening, protrusive movement, lateral movement, clenching and occlusion was quantified. Results:A correlation was observed between synovitis and temporomandibular arthralgia (p<0.01). Synovitis occurred most often in the posterior section, followed by the posterior region of the anterior section, and anterior section, respectively. Anterior synovitis correlated to pain during mouth opening, protrusive movement, lateral movement of the affected side, and lateral movement of the normal side. Posterior synovitis was associated with pain during mouth opening, protrusive movement, lateral movement of the affected side and occlusion. Degree of arthralgia during mouth opening associated with posterior synovitis was significantly worse than that associated with anterior synovitis (p<0.05). However, the degree of correlation between synovitis and pain at rest and during clenching was low.
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  • 高橋 徳明
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 166-177
    公開日: 2017/03/21
    ジャーナル フリー
    We have previously reported that chick serum (CKS) strongly induced adipogenesis in Meckel's chondrocyte in vitro, but little is known about the effects on other mesenchymal cells. In the present study, CKS-induced adipogenesis on the osteogenic MC3T3-E1 cells, mouse subcutaneous fibroblasts (MSF), and undifferentiated mouse dental pulp cells (MDP) were examined using light and electron microscopy, immunofluorescence staining, and BrdU-incorporation for DNA-synthesizing cells. Furthermore, MSF was cultured on a collagen-gel matrix that allowed maintenance of three-dimensional integrity. CKS facilitated lipogenesis and induced the formation of lipid droplets in all kinds of cells. Immunostaining showed the expression of peroxisome proliferator-activated receptor-γ in immature adipocytes, and leptin and glucose transporter-4 in mature adipocytes. Adipocytes on collagen-gel matrix induced unilocular lipid droplets at a high frequency. These morphological observations strongly indicate that these lipid-rich cells retain sufficient features as intact adipocytes, although the adipocytes that were formed in the present study were multilocular adipocytes. Furthermore, it was suggested that CKS agents involved in adipocyte differentiation are a common inductive factor affecting various mesenchymal cells.
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  • 鶴田 摂
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 178-186
    公開日: 2017/03/21
    ジャーナル フリー
    An objective methodology of color reproduction based on color science has been attempted for dental restoration. The purpose of this study was to evaluate the accuracy in reproducing a tooth color gradation in ceramic by means of a Computer Color Matching (CCM) system employing a prototype program and spectrophotometer measurements. The colors of 5 shade tabs of VITAPAN 3D MASTER were measured for 4 regions of each tooth from the incisal to the gingival area. Ceramic prescriptions calculated by CCM program were used to fabricate tooth shaped ceramic specimens using IPS Empress 2. Formulated ceramic powders were applied to the color regions and blended at the interfaces for shade continuity. Color coordinates L^*, a^* and b^* of ceramic specimens and target shade tabs for each pair in 10 incremental areas (each 1 mm x 3 mm) indicated very similar values. Color difference (dE) were below 3.6 in all 10 incremental areas. It was concluded that the CCM methodology reproduced the target tooth color gradation at a clinically satisfactory level.
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  • 昆 麻子
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 187-196
    公開日: 2017/03/21
    ジャーナル フリー
    Porphyromonas endodontalis, a gram-negative anaerobic rod, is an important pathogenic organism in periapical lesions with acute symptoms, such as pain, swelling, and suppuration in endodontic patient. Like P. gingivalis, a major pathogen of adult periodontitis, P. endodontalis is asaccharolytic and forms black-pigmented colonies on enriched blood agar plates. However, pathogenic factors and pathological potential of this microbe have been poorly characterized. In this study, we assessed the adhesive abilities of P. endodontalis to gingival epithelial cell, and investigated whether P. endodontalis has a major fimbrial component of fimbrilin similar to that of P. gingivalis. Furthermore, the proteolytic activities of P. endodontalis were examined. The results indicated that P. endodontalis significantly adhered to a mouse gingival epithelial cell line, GE1, although the adhesive ability was lower than that of P. gingivalis. The electron microscopic investigations showed scarcely any fimbriae in P. endodontalis. Furthermore, PCR and the DNA-DNA hybridization analysis for the gene encoding fimbrilin (fimA) of P. gingivalis revealed that P. endodontalis did not have fimA or fimA-related genes, which could account for the lesser adhesive ability of P. endodontalis to gingival epithelial cells. The hydrolyzing profile toward various synthetic peptides showed that the spectrum of proteolytic activities of P. endodontalis was markedly different from that of P. gingivalis. Thus, the present findings indicate that P. endodontalis has a different adhesion mechanism from P. gingivalis fimbriae, and has a different spectrum of proteolytic activities from that of P. gingivalis, which may lead to the specific pathological potential of the organisms in periapical lesions in humans.
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  • 稲葉 大輔, 南 健太郎, 釜阪 寛, 米満 正美
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 197-202
    公開日: 2017/03/21
    ジャーナル フリー
    著者らは,馬鈴薯澱粉の加水分解物より調製されるリン酸化オリゴ糖(POs)がカルシウムの溶解を強く促進し,カルシウム-リン酸の沈殿形成を阻害することを報告した。本研究の目的はエナメル質の齲蝕様病巣の再石灰化におよぼすPOsの効果をin vitroで検討することである。ウシエナメル質試料を0.1M乳酸ゲル(pH5)で脱灰後,NaFとして2ppmFまたは様々な濃度のPOs(0.07-4%)を添加したミネラル溶液(20mM Hepes,1.5mM CaCl_2,0.9mM KH_2PO_4,pH7)に37℃で7日間,浸漬した。エナメル質のミネラル濃度分布はtransversal microradiographyにより定量化した。0.07-0.2%のPOsを含むミネラル溶液で処理した試料のミネラル喪失量(ΔZ,vol%・μm)は,脱灰エナメル質よりも有意に低く(p<0.001),しかも2ppmF群と統計学的に同等の値であった。POs群試料の再石灰化率はPOs濃度の対数値と有意な負の相関を示した(r=0.99,p<0.0001)。本研究の結果から,POsは新規かつユニークなエナメル質の再石灰化促進物質であり,栄養学的なアプローチにより齲蝕予防に活用しうる可能性が示唆された。
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  • 稲葉 大輔, 南 健太郎, 釜阪 寛, 米満 正美
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 203-209
    公開日: 2017/03/21
    ジャーナル フリー
    著者らはリン酸化オリゴ糖カルシウム塩(POs-Ca)が馬鈴薯澱粉の加水分解物より抽出でき,カルシウムの溶解性を高めることを先に報告した。本研究の目的は,POs-Ca配合シュガーレスガムのエナメル質および象牙質の再石灰化におよぼす効果を口腔内実験により確認することである。12名のボランティア(男性6名,女性6名;平均22歳)をランダムに2群に分け,二重盲検,クロスオーバーデザインの口腔内実験を行った。各被験者は脱灰した牛歯エナメル質および象牙質ディスクを取り付けた上顎口蓋プレートを装着し,キシリトールガムまたは2.6%POs-Ca配合キシリトールガムのいずれかを,1日4回噛んだ(期間:2週間/ガム)。実験期間中,フッ化物は使用せず,エナメル質ディスクが乾燥しないよう注意した。エナメル質ディスクのマイクロラジオグラフから脱灰深度ldを評価した。POs-Caガム群のエナメル質および象牙質のld値は,それぞれ70±11μmならびに71±13μm(Mean±SD)で,キシリトールガムの値(エナメル質:101±21μm,象牙質:95±13μm)よりも25-31%減少していた(p<0.001)。このミネラル蓄積のメカニズムは,POs-Caが唾液のCa/P比をハイドロキシアパタイトの比率(1.67)に向けて高め,歯質に対して過飽和なミネラル状態を維持させたことによると考えられた。本研究より,2.6%POs-Ca配合シュガーレスガムを毎日利用することは,エナメル質と象牙質,双方の再石灰化を強く促進し,齲蝕予防に有効であることが示唆された。
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  • 間山 寿代
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 210-218
    公開日: 2017/03/21
    ジャーナル フリー
    Using photodensitometry for 47 patients who had congenitally missing permanent teeth, excluding for third molars, bone mineral content (BMC), determined via dental radiographs, was compared with a control group of 43 patients who have all their teeth. In addition, bone age was determined by hand-wrist radiographs using the TW 2 method, and the differences of skeletal maturation between both groups were evaluated. The results are as follows : 1. The average BMC was significantly lower in the group missing teeth than in the control group both for young males (aged 7-15 years old) and young females (aged 6-15 years old). 2. The BMC in the group of people missing more than three teeth was significantly lower than those in the groups of people missing one or two teeth. 3. Regarding the average BMC by the location of missing teeth : The maxillo-mandibular group had the lowest BMC, followed by the second lowest mandible group and finally the maxillary group. By type of missing teeth : The molar, which contained at least one molar and anterior-premolar teeth group, had the lowest BMC. Second lowest was the group missing premolar followed by the group missing anterior. 4. In the groups with missing teeth, bone age was lower by 0.97 years in young males compared to 1.18 years in young females. These results show delayed skeletal maturation. In conclusion, this study clearly indicates that compared to the control group BMC is lower, and skeletal maturity has a tendency to be delayed in children with missing teeth. These results can assist planning of orthodontic treatment to patients with congenitally missing teeth.
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  • 畠山 忠臣, 佐藤 健一
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 219-229
    公開日: 2017/03/21
    ジャーナル フリー
    The purpose of this study was to clarify the effects and mechanisms of lidocaine (1.0×10-3M)on changes in contraction of the smooth muscle induced by voltage dependent Ca2+ channel (VDCC) stimulator, KCl(90mM), and receptor activated Ca2+ channel (RACC) agonist, adrenaline(2×10-5M), in porcine lingual arteries.
    The isometric tension and intracellular Ca2+ concentration ( [Ca2+]i) by the fura-2 microfluorometric methods were measured simultaneously, and from them we tried to deduce the depressing mechanism of lidocaine (1.0×10-3M) on the contraction.
    The results were obtained as follows:
    (1) Lidocaine depressed the increase of contraction and [Ca2+]i induced by KCl and adrenaline in a concentration-dependent manner.
    (2) Lidocaine depressed the increase of contraction and [Ca2+]i induced by adrenaline in normal physiological salt solution after depletion of the intracellular Ca2+-sensitive Ca2+ store.
    (3) Lidocaine depressed the increase of contraction and [Ca2+]i induced by adrenaline in Ca2+-free physiological salt solution.
    (4) Lidocaine had no effect on Ca2+ induced Ca2+ release(CICR) by caffein. These results suggest the following conclusions as follows,
    1. Lidocaine depresses influx of Ca2+ through VDCC and RACC. 2. Lidocaine inhibits the increase of [Ca2+]i through IP3 processes. 3. Lidocaine has no effect on CICR.
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  • 遠藤 忠治
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 230-236
    公開日: 2017/03/21
    ジャーナル フリー
    The color of natural teeth is semi-translucent enamel with a high transparency and chromatic dentin. The purpose of this study is to analyze the optical properties of human dentin and the relationship between the color spectrum and metal elements in the dentin. 120 specimens of human dentin sample with a thickness of 0.5 mm (±0.05 mm) were made. Reflectance values were measured by a spectrophotometer CAS-ID 1 with a white backing and a black backing. Scattering coefficients, absorption coefficients, R-infinity, CIELAB color coordinates L*, a*, b* and Color depth SQ were calculated based on each reflectance value. Metal elements in dentin were analyzed by electron probe X-ray microanalyzer (EPMA) and Fe, Zn, Mg, Cu and Ni in 10 areas of each dentin specimen were quantified and averaged. Mean L* value of dentin was 11.6±4.7, 29.6±27.3 for SQ, 70.1±11.3 for a*, 11.6±4.3 for C* and -0.1±2.1 for b*. Ni indicated a positive correlation with a* and b* value, Zn showed a positive correlation with L* value. On the other hand, Mg indicated a negative correlation with a* value. It was concluded that those three metal elements could create the dentin color defined in this study.
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  • 大平 明範, 佐藤 理恵, 根反 不二生, 関山 三郎
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 237-245
    公開日: 2017/03/21
    ジャーナル フリー
    Regarding improved range of mouth opening for patients with temporoman-dibular joint arthrosis, improvements in elasticity of the upper joint cavity and relaxation of the articular capsule are believed to be involved. When the maxillary joint cavity is pressurized and irrigated, it expands, thus increasing volume. In the present study, to investigate the relationship between range of mouth opening and elasticity of the joint cavity in temporo-mandibular joint arthrosis, elasticity of the temporomandibular joint cavity was assessed mechanically based on volume of the upper joint cavity and changes in outflow before and after temporoman-dibular joint cavity irrigation. In addition, the relationship between these mechanical parameters and changes in range of mouth opening before and after irrigation was investigated. While outflow increased after irrigation in some patients with temporomandibular joint arthrosis (I type), it decreased after irrigation in others (D type). Of the 60 joints studied, 43 Type I joints (71.7%) were identified. Sufficient improvements in range of mouth opening could not be achieved when the elasticity and volume of the upper joint cavity were small and when relaxation of the upper joint cavity was seen after PLS.
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  • 村田 純一郎, 松本 範雄
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 246-260
    公開日: 2017/03/21
    ジャーナル フリー
    We have shown that conditioning stimulation of the amygdaloid nucleus has an inhibitory effect on the nociceptive responses of the somatosensory cortex. The purpose of this study is to investigate whether amygdaloid conditioning stimulation exerts influence on the nociceptive neurons in the medullary dorsal horn in the rat. The animals were anesthetized with N_2O-O_2 (2 : 1) and 0.5%-halothane, and immobilized with pancuronium bromide. The peripheral test stimulus (a single rectangular pulse of 2.0 msec in duration) was applied to the receptive field of nociceptive neurons, and the ipsilateral amygdaloid conditioning stimulation were trains of 33 pulses (0.5 msec in duration, 300 μA) delivered at 330Hz. Fifty-five WDR neurons were distributed in the superficial layers of the caudal nucleus and diffusely throughout the reticular subnucleus, whereas most of NS neurons (13/17) were distributed in the dorsal part of reticular subnucleus. The conditioning stimulation in the central nucleus, basomedial nucleus and basolateral nucleus, markedly inhibited the activities in 24 of 43 nociceptive neurons. The inhibitory effect was 45.3±16.1% (n=24) at maximum. The conditioning stimulation had almost no effect on the discharges of non-nociceptive neurons. In contrast, the conditioning stimulation in the lateral amygdaloid nucleus had no effect on the nociceptive and non-nociceptive neurons. The present results suggest that amygdaloid nucleus inhibits the ascending nociceptive information at the second order neurons. This amygdaloid antinociceptive effect may provide one of the neurophysiological bases for the stress-induced analgesia (SIA) observed in the presence of stress and fear.
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  • 及川 ありさ
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 261-269
    公開日: 2017/03/21
    ジャーナル フリー
    The functional role of CD14 as well as Toll-like receptors (TLRs) in the Porphyromonas gingivalis (P. gingivalis) LPS-induced activation of B cells was assessed, using CD14-, TLR2- and/or TLR4-overexpressed murine B cells (CH12.LX). After stimulation with P. gingivalis LPS, both CD14- and TLR4-transfected (but not TLR2-transfected) CH12.LX cells showed higher induction of NF-κB activation than a parent vector-transfected cells, suggesting that P. gingivalis LPS could induce B cell activation in a CD14-dependent pathway through TLR4 and NF-κB activation. Furthermore, the control CH12.LX cells proliferated under stimulation with P. gingivalis LPS, accompanied by up-regulations of transforming growth factor (TGF)-β and intereleukin (IL)-6 mRNA. In contrast, the overexpression of CD14 resulted in enhancement of the up-regulations of TGF-β mRNA, but not IL-6 mRNA or proliferative responses to P. gingivalis LPS. These results suggest that P. gingivalis LPS could induce B cell activation both in CD14-dependent and independent pathways. In a CD14-dependent pathway, TGF-β production could be induced upon stimulation with P. gingivalis LPS through TLR4 and NF-κB activation. A CD14-independent pathway could also exist in the P. gingivalis LPS-induced activation of B cells that leads to proliferation and IL-6 production.
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  • 守口 斉, 小豆島 正典
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 270-278
    公開日: 2017/03/21
    ジャーナル フリー
    Positron emission tomography using fluorine-18 fluoro-deoxyglucose (FDG PET) is useful for the detection of malignant tumor recurrences and for the evaluation of a therapeutic response to these;including ones found in the lung, colon, head and neck regions. The experimental study demonstrated FDG uptake was higher in faster-growing rather than in slower-growing tumors. These findings show FDG accumulation exhibits cell cycle dependency. However, the precise mechanism remains to be elucidated. In this study, the relationship between FDG uptake and the cell cycle phase in HeLa S3 cells, as well as how they compare to the conventional tracer 67Ga citrate (Ga) with single photon emission tomography (SPECT) was assessed. Synchronization of HeLa S3 cells was accomplished via a double thymidine block. The uptake of FDG and Ga was determined after cell cycle synchronization. The glucose transporter was independently evaluated for the level of its Glut 1 glucose transporter membrane protein. FDG uptake in HeLa S3 cells was significantly higher in the early S phase and G2/M phase compared to the G1 phase. In addition, Ga uptake was higher in the G2/M phase. Immunochemical assays for the Glut 1 transporter showed an increase in membrane expression within S phase cells. It has been concluded that cell cycle dependency is reflected in the uptake of FDG and Ga, seen during PET or SPECT imaging of tumor tissue. These results reveal tumor proliferative activity, and can assist in evaluating a therapeutic response. Furthermore, the increased FDG uptake, in part due to increased membrane expression of the Glut 1 glucose transporter, contributes to this phenomenon.
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  • 清藤 洋之, 小川 淳, 關 聖太郎, 福田 喜安, 水城 春美, 武田 泰典, 佐藤 方信
    原稿種別: 本文
    27 巻 (2002) 3 号 p. 279-283
    公開日: 2017/03/21
    ジャーナル フリー
    Minor salivary gland tumors were clinicopathologically evaluated. Between 1990 and 2000, 31 patients with minor salivary gland tumors (10 males and 21 females between 12 and 75 years of age, mean age : 49.9 years) were treated in our department. Minor salivary gland tumors most frequently occurred in the palate in 19 patients, followed by 5 in the buccal mucosa, 4 in the lips, and 1 in the oropharynx. Histopathological examinations revealed 20 benign and 11 malignant tumors. Pleomorphic adenoma accounted for the majority (19 cases) of the benign tumors. Malignant tumors consisted of 3 mucoepidermoid carcinomas, 3 adenoid cystic carcinomas, 1 adenocarcinoma, 1 acinic cell carcinoma, 1 salivary duct carcinoma, 1 tubular adenocarcinoma, and 1 basal cell adenocarcinoma. All cases of benign tumors were successfully treated by extirpation without tumor recurrence. Of the 11 cases of malignant tumors, 7 were treated by surgery alone, 1 was treated by surgery after chemotherapy, and 3 were treated by surgery after chemotherapy and radiotherapy. Primary lesions recurred in 2 of these patients. The outcomes of patients with malignant tumors were as follows : 6 patients survived without other complications, 3 died of uncontrollable tumors, and 2 died of other diseases. Of the 3 patients who died of uncontrollable tumors, 2 died of primary lesions and 1 died of cervical lymph node metastasis.
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