Starch granules were prepared from mature grains of 75 cultivars (23
indica, 27
Chinese indica, 6
japonica and 19
javanica) of rice originating in Asia and the other countries, including Brazil (4), China (25), India (10), Indonesia (3), Japan (8), Korea (2), Laos (3), Myanmar (3), Nepal (4), Pakistan (1), the Philippines (1), Russia (1), Taiwan (3), Thailand (1) and the USA (6). They were cultivated and harvested in the paddy field of Prefectural University of Hiroshima in 2001. We showed that starches of non-waxy cultivars of the
indica and
Chinese indica, in general, had higher contents of the apparent amylose (AAM) and super-long chains (SLC) of amylopectin by GPC of
Pseudomonas isoamylase-debranched starches and amylopectins through Toyopearl columns. High performance anion exchange chromatography with a pulsed amperometric detection (HPAEC-PAD) of isoamylase-debranched starches showed that the starches of non-waxy cultivars of the
indica and
Chinese indica, in general, had decreased amounts of branch chains with DP 6-12 (Fr. A). The Fr. A contents correlated positively with the alkali spreading score (ASS) of rice grains and negatively with the peak temperature (
Tp) of gelatinization of the rice starches. Among the pasting characteristics of the starches measured using a Rapid Visco Analyser (RVA), setback (
SB) and breakdown (
BD) showed high positive and negative correlations with SLC contents, respectively, and both peak top viscosity (
PV) and
BD negatively correlated to AAM contents. There was a high positive relationship between amounts of Waxy (
Wx) protein and SLC contents in starch. This appears to show that
Wx protein is concerned with synthesis of SLC. SLC contents in starches of rice originating in Asia and the other countries were evenly observed in the range of 0.0-13.4%.
View full abstract